Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Accumulation of mtDNA Deletion (${\Delta}mtDNA^{4977}$) showing Tissue-Specific and Age-Related Variation

조직별 및 나이에 따른 마이토콘드리아 DNA 결손 (${\Delta}mtDNA^{4977}$)의 축적

  • Jeong, Hye-Jin (Genome Research Center for Reproductive Medicine and Infertility of KOREA NIH) ;
  • Chung, Hyung-Min (Cell and Gene Theraphy Research Institute, Pochon CHA University) ;
  • Cho, Sung-Won (Genome Research Center for Reproductive Medicine and Infertility of KOREA NIH) ;
  • Kim, Hyun-Ah (Genome Research Center for Reproductive Medicine and Infertility of KOREA NIH) ;
  • Lee, Kyung-Sool (Department of Obstetrics and Gynecology, CHA General Hospital College of Medicine, Pochon CHA University) ;
  • Kwon, Hwang (Department of Obstetrics and Gynecology, CHA General Hospital College of Medicine, Pochon CHA University) ;
  • Choi, Dong-Hee (Department of Obstetrics and Gynecology, CHA General Hospital College of Medicine, Pochon CHA University) ;
  • Kwak, In-Pyung (Department of Obstetrics and Gynecology, CHA General Hospital College of Medicine, Pochon CHA University) ;
  • Yoon, Tae-Ki (Department of Obstetrics and Gynecology, CHA General Hospital College of Medicine, Pochon CHA University) ;
  • Lee, Sook-Hwan (Department of Obstetrics and Gynecology, CHA General Hospital College of Medicine, Pochon CHA University)
  • 정혜진 (보건복지부지정 생식의학 및 불임 유전체 연구센터) ;
  • 정형민 (포천중문의대 세포유전자치료연구소) ;
  • 조성원 (보건복지부지정 생식의학 및 불임 유전체 연구센터) ;
  • 김현아 (보건복지부지정 생식의학 및 불임 유전체 연구센터) ;
  • 이경술 (포천중문의대 차병원 산부인과) ;
  • 권황 (포천중문의대 차병원 산부인과) ;
  • 최동희 (포천중문의대 차병원 산부인과) ;
  • 곽인평 (포천중문의대 차병원 산부인과) ;
  • 윤태기 (포천중문의대 차병원 산부인과) ;
  • 이숙환 (포천중문의대 차병원 산부인과)
  • Published : 2003.09.30
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Abstract

Objectives: Controversial arguments exists on both the case for and against on the accumulation of mitochondrial DNA (mtDNA) deletion in association to tissue and age. The debate continues as to whether this mutation is a major contributor to the phenotypic expression of aging and common degenerative diseases or simply a clinical insignificant epiphenomenon. The objective of this study was to determine whether the accumulation of mtDNA deletion is correlated with age-related and tissue-specific variation. Materials and Methods: One hundred and fifty-seven tissues from blood, ovary, uterine muscle, and abdominal muscle were obtained from patients ranging in age from 31$\sim$60 years. After reviewing the clinical reports, patients with mitochondrial disorder were excluded from this study. The tissues were obtained at gynecological surgeries with the consent of the patient. Total DNA isolated from blood, ovary, uterine muscle, and abdominal muscle was amplified by two rounds of PCR using two pairs of primers corresponding to positions 8225-8247 (sense), 13551-13574 (antisense) for the area around deleted mtDNA and 8421-8440 (sense), 13520-13501 (antisense) for nested PCR product. A statistical analysis was performed by $x^2$-test. Results: About 0% of blood, 94.8% of ovary, 71.4% of uterine muscle, and 86.1% abdominal muscle harbored mtDNA deletion. When we examined the proportion of deleted mtDNA according to age deletion rate was 90% of ovary, 63.6% of uterine muscle, 77.7% of abdominal muscle in thirties and 100% of all tissue in fifties. Conclusion: The findings of this study suggest that the mtDNA deletion is varied in tissue-specific pattern and increases with aging.

Keywords

References

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