뼈모세포의 활성도에 대한 자성의 효과에 관한 연구

The Effect of Magnetism(Neodymium Magnet) on Activity of Osteoblast

The object of this study is to observe the effects of magnetism on the osteoblasts using a neodymium magnet. The osteoblasts was cultured under magnetic fields of varying intensities to evaluate the effect of magnetism on the activity and alkaline phosphatase acitivty of the osteoblasts. Osteoblasts were cultured in the cell density of $10^4$ for the evaluation of cell proliferation and 105/ml for the evaluation of ALP activity under 0. 10, 100, 500, 1000, 2000, 4000 gauss for 24 hour. For evaluation of osteoblast morphologic changes under magnetic, osteoblasts were observed by inverted microscope and TEM. To elucidate if IGF-receptors are increased under the magnetic field, we investigated osteoblasts by immunofluoroscence staining. The results were as follows: In the varying intensities of magnetic fields, the degree of cell proliferation was the highest in the magnetic field of 10 gauss and this gradually decreased up to 1000 gauss. In the magnetic fields stronger than 1000 gauss, the degree of the cell proliferation decreased to an even lower level than that of the control group. The ALP activity and protein synthesis showed a similar increase pattern as the degree of cell proliferation compared to the control group but showed little difference. Under the microscope, morphological change of the cells ( decrease in length and increase in roundness) were observed but no peculiarity of cell distribution could be found according to the magnetic field line. In the proper intensity of magnetic fields (10 gauss), the cultured cells showed increase in number of IGF Receptors compared to that of the control group.