Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Plant Growth Promotion in Soil by Some Inoculated Microorganisms

  • Jeon, Jong-Soo (Division of Biological Sciences Kangwon National University) ;
  • Lee, Sang-Soo (Division of Biological Sciences Kangwon National University) ;
  • Kim, Hyoun-Young (Division of Biological Sciences Kangwon National University) ;
  • Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University) ;
  • Song, Hong-Gyu (Division of Biological Sciences Kangwon National University)
  • Published : 2003.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

The inoculation of some microorganisms into a microcosm containing soil from a barren lakeside area at Lake Paro in Kangwon-do enhanced plant growth significantly. The direct and viable counts of soil bacteria and soil microbial activities measured by electron transport system assay and fluorescein diacetate hydrolysis assay were higher in inoculated soil. The plant growth promoting effect of this inoculation may be caused by phytohormone production and the solubilization of insoluble phosphates by the inoculated bacteria. Three inoculated strains of Pseudomonas fluorescens produced several plant growth promoting phytohormones, including indole-3-acetic acid (auxin), which was confirmed by thin layer chromatography and GC/MS. P. fluorescens strain B16 and M45 produced 502.4 and 206.1 mg/l of soluble phosphate from Ca3(PO4)2 and hydroxyapatite, respectively. Bacillus megaterium showed similar solubilization rates of insoluble phosphates to those of Pseudomonas spp. We believe that this plant growth promoting capability may be used for the rapid revegetation of barren or disturbed land.

Keywords

References

  1. Ahn, T., J. Lee, D. Lee, and H. Song. 2002. Ecological monitoring of soil microbial community after forest fire. Proceeding of Symposium on Prevention of large forest fire and remediation of ecosystem. p. 144-175. Korea Forest Research Institute.
  2. Atlas, R. and R. Bartha. 1998. Microbial Ecology: Fundamentals and Applications, p. 99-103. Benjamin/Cummings Sci. Pub., Menlo Park, CA.
  3. Bashan, Y. 1998. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnol. Adv. 16, 729-770.
  4. Clesceri, L.S., A.E. Greenberg, and A.D. Eaton. 1998. Standard methods for the examination of water and wastewater, 20th ed. APHA-AWWA-WEF. Washington, D.C.
  5. Garbaye, J. and G.D. Bowen. 1989. Stimulation of mycorrhizal infection of Pinus radiata by some microorganisms associated with the mantle of ectomycorrhizas. New Phytologist 122, 383-388.
  6. Gamalero, E., L. Fracchia, M. Cavaletto, J. Garbaye, P. Frey-Klett, G.C. Varese, and M.G. Martinotti. 2003. Characterization of functional traits of two fluorescent pseudomonads isolated from basidiomes of ectomycorrhizal fungi. Soil Biol. Biochem. 35, 55-65. https://doi.org/10.1016/S0038-0717(02)00236-5
  7. Gerhardson, B. and S. Wright. 2002. Bacterial associations with plants: Beneficial, non N-fixing interactions. p. 79-103. In K. Sivasithamparam, K.W. Dixon, and R.L. Narrett (eds.) Microorganism in Plant Conservation and Biodiversity. Kluwer Academic Press, London.
  8. Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Can. J. Microbiol. 41, 109-117.
  9. Kim, K.Y., D. Jordan, and H.B. Krishnan. 1997. Rahnella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite. FEMS Microbiol. Lett. 153, 273-277.
  10. Koga, J. 1995. Structure and function of indolepyruvate decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis. Biochim. Biophys. Acta 1249, 1-13.
  11. Lund, V. and J. Goksøyr. 1980. Effects of water fluctuations on microbial mass and activity in soil. Microb. Ecol. 6, 115-123.
  12. Misko, A.L. and J.J. Germida. 2002. Taxonomic and functional diversity of pseudomonads isolated from the roots of fieldgrown canola. FEMS Microbiol. Ecol. 42, 399-407.
  13. Mordukhova, A.E., S.L. Sokolov, V.V. Kochetkov, A.I. Kosheleva, F.N. Zelenkova, and A.M. Boronin. 2000. Involvement of naphthalene dioxygenase in indole-3-acetic acid biosynthesis by Pseudomonas putida. FEMS Microbiol. Lett. 190, 279-285.
  14. Normander, B., N.B. Hendriksen, and O. Nybroe. 1999. Green fluorescent protein-marked Pseudomonas fluorescens: localization, viability, and activity in the natural barley rhizosphere. Appl. Environ. Microbiol. 65, 4646-4651.
  15. Patten, C.L. and B.R. Glick. 2002. Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl. Environ. Microbiol. 68, 3795-3801.
  16. Pilet, P.E. and M. Saugy. 1987. Effect on root growth of endogenous and applied IAA and ABA. Plant Physiol. 83, 33-38.
  17. Prosser, J.I. 1997. Microbial processes within the soil. p. 183-213. In J.D. van Elsas, J.T. Trevors, and E.M.H. Wellington (eds.) Modern soil microbiology. Marcel Dekker, Inc., New York.
  18. Rodríguez, H. and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17, 319-339.
  19. Son, H.-J., Y.-G. Kim, and S.-J. Lee. 2003. Isolation, identification and physiological characteristics of biofertilizer resources, insoluble phosphate-solubilizing bacteria. Kor. J. Microbiol. 39, 51-55.
  20. Sorensen, J., L.E. Jensen, and O. Nybroe. 2001. Soil and rhizosphere as habitats for Pseudomonas inoculants: new knowledge on distribution, activity and physiological state derived from micro-scale and single-cell studies. Plant Soil 232, 97-108.
  21. Tate, R.L. 2000. Soil Microbiology, p. 149-152. John Wiley & Sons, Inc. New York.
  22. Whitelaw, M.A., T.J. Harden, and K.R. Helyar. 1999, Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol. Biochem. 31, 655-665.