Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effect of Conjugated Linoleic Acid(CLA) Feeding Levels and Periods on Textural Property and Fatty Acid Composition of Pork

Conjugated Linoleic Acid(CLA) 급여수준과 급여기간이 돈육의 조직감과 지방산 조성에 미치는영향

  • Lee, J.I. (Gyeongnam Province Advanced Swine Research Institute) ;
  • Ha, Y.J. (Gyeongnam Province Advanced Swine Research Institute) ;
  • Kwack, S.C. (Gyeongnam Province Advanced Swine Research Institute) ;
  • Lee, J.D. (Gyeongnam Province Advanced Swine Research Institute) ;
  • Kim, D.H. (Regional Animal Industry Research Center, Jinju National University) ;
  • Kang, G.H. (Division of Animal Science, College of Agriculture, Gyeongsang National University) ;
  • Hur, S.J. (Division of Animal Science, College of Agriculture, Gyeongsang National University) ;
  • Park, G.B. (Division of Animal Science, College of Agriculture, Gyeongsang National University)
  • 이정일 (경상남도 첨단양돈연구소) ;
  • 하영주 (경상남도 첨단양돈연구소) ;
  • 곽석준 (경상남도 첨단양돈연구소) ;
  • 이중동 (경상남도 첨단양돈연구소) ;
  • 김두환 (진주산업대학교 동물생명산업지역협력연구센터) ;
  • 강근호 (경상대학교 축산과학부) ;
  • 허선진 (경상대학교 축산과학부) ;
  • 박구부 (경상대학교 축산과학부)
  • Published : 2003.12.31
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Abstract

To investigate the effects of conjugated linoleic acid added diet feeding on CLA accumulation and quality characteristics of pork meat. The CLA used to add in diet was chemically synthesized by alkaline isomerization method with corn oil. Pigs were divided into 5 treatment groups(4 pigs/group) and subjected to one of five treatment diets(0, 1.25% CLA for 2 weeks, 2.5% CLA for 2 weeks, 1.25% CLA for 4 weeks and 2.5% CLA for 4 weeks, CLA diets; total fed diets) before slaughter. Pork loin were collected from the animals(110 kg body weight) slaughtering at the commercial slaughter house. Pork loin meat were aerobic packaged and then stored during 2, 5, 8, 11 and 14 days at 4$^{\circ}C$. Samples were analyzed for shear force value, texture, TBARS, fatty acid composition, cholesterol and CLA content. CLA treatment groups showed significantly(p〈0.05) higher shear force value compared to those of control group at 11, 14 days of cold storage. All treatments were decreased significantly as the storage period passed. There was a not significantly difference in texture between control and CLA treatment groups. All CLA treatment groups showed significantly(p〈0.05) lower TBARS value than the control. TBARS value was increased significantly during storage in all treatment. CLA treatment groups showed significantly(p〈0.05) lower cholesterol content compared to those of control group. As dietary CLA was increased in feed, the content of CLA was increased, but the control was almost not detected. The contents of CLA were not significantly changed during chilled storage for 14 days. In the change of fatty acid composition, the contents of oleic, linoleic and arachidonic were decreased by dietary CLA-supplementation, whereas the increase level of CLA-supplementation resulted in the higher palmitic and stearic acid. In all results, CLA could be accumulated in pork meat and its antioxidant capability has been indicated. It was suggested that dietary CLA-supplementation could be produced high quality pork.

Conjugated linoleic acid(CLA)를 비육돈에 급여하여 비육돈 체조직내에 CLA를 축적시키고, CLA가 축적된 돈육의 품질특성에 대한 연구를 수행하였다. CLA는 alkaline isomerization 방법으로 식용유를 이용하여 전체 CLA 이성체가 50%인 CLA를 화학적으로 합성하였다. 대조구는 80${\sim}$110 kg 까지는 일반사료회사에서 생산하는 사료를 급여하였으며, 처리구 1은 출하(출하체중:110 kg) 2주전부터 일반사료회사에서 생산하는 사료에 CLA를 1.25% 첨가하여 급여하였으며, 처리구 2는 출하 2주전부터 사료 함량에 CLA를 2.5% 첨가하여 급여하였다. 처리구 3은 사료 함량에 1.25% CLA를 첨가하여 4주간 급여하였으며, 처리구 4는 2.5% CLA를 4주간 급여하였다. 급여기간이 끝난 후 일괄적으로 함양도축장에서 도축하여 돈육의 등심부위를 함기포장하여 냉장온도(4$^{\circ}C$)에서 14일간 저장하면서 각 실험항목을 조사하였다. 저장기간에 따른 돈육등심의 CLA 함량, 콜레스테롤, 지방산 조성, 지방산화, 조직감 및 연도 등을 조사하여 CLA가 축적된 돈육의 저장기간에 따른 품질특성을 규명하고자 하였다. CLA 급여수준과 급여기간이 전단가에 미치는 영향을 조사한 결과 처리구간의 비교에서 저장 8일 까지는 처리구간에 유의적인 차이가 없었지만 저장 11일과 14일에는 대조구가 CLA 급여 처리구에 비하여 유의적으로 낮은 전단가를 보였다(P〈0.05). 모든 처리구들이 저장기간이 경과함에 따라 유의적으로 낮아지는 결과를 보였다(P〈0.05). 경도는 전 저장기간 동안 처리구간의 비교에서 대조구와 CLA 급여 처리구간에는 유의적인 차이가 없었으며, 저장기간의 경과에 따른 변화는 모든 처리구가 저장기간이 경과함에 따라 감소하는 경향을 보였다. 점착성은 대조구와 CLA 급여 처리구간에 뚜렷하게 차이나는 경향이 없고 저장기간이 경과함에 따라 뚜렷한 변화가 없었다. TBARS 변화는 전 저장기간동안 처리구간의 비교에서 CLA를 급여하지 않은 대조구에 비하여 CLA를 급여한 처리구가 유의적으로 낮은 TBARS를 보였다(P〈0.05). 저장기간에 따른 비교에서는 모든 처리구가 저장기간이 경과함에 따라 유의적으로 증가하였다(P〈0.05). 콜레스테롤 함량은 저장 2일에 대조구와 비교해서 CLA 급여 처리구가 유의적으로 낮은 콜레스테롤 함량을 나타내었으며(P〈0.05), T4 처리구가 가장 낮은 콜레스테롤 함량을 나타내었다(P〈0.05). 지방산 조성은 전 저장기간동안 CLA 급여수준과 급여기간이 증가할수록 myristic, palmitic 및 stearic acid 함량은 유의적으로 증가하며(P〈0.05), 반면에 oleic, linoleic 및 arachidonic acid 함량은 유의적으로 감소하였다(P〈0.05). CLA 함량을 보면 CLA를 급여하지 않은 대조구는 CLA가 검출되지 않았지만 CLA 급여 처리구에서는 CLA 급여수준과 급여기간이 증가할수록 육내 축적율이 직선적으로 증가하였다. 이상의 결과를 종합하여 보면 CLA를 육내에 축적시킬 수 있으며, CLA가 축적된 돈육은 저장 중 지방산화를 억제하는 항산화 능력이 있어 고품질 . 고기능성 돈육생산이 가능하다고 사료된다.

Keywords

References

  1. Beuge, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52:302-303. https://doi.org/10.1016/S0076-6879(78)52032-6
  2. Chen, Z. Y., Chan, P. T., Kwan, K. Y. and Zhang, A. 1997. Reassessment of the antioxidant activity of conjugated linoleic acids. JAOCS. 73:749-753.
  3. Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L. and Pariza, M. W. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recogenized class of anticarcinogens. J. Food Composition and Analysis. 5:185-197. https://doi.org/10.1016/0889-1575(92)90037-K
  4. Chin, S. F., Storkso, J. M., Albright, K. J., Cooke, M. E. and Pariza, M. W. 1994. Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. J. Nutr. 124:2344-2349.
  5. Cook, M. E., Miller, C. C., Park, Y. and Pariza, M. W. 1993. Immune modulation by altered nutrient metabolism: nutritional control of immune induced growth depression. Poult. Sci. 72:1301-1305. https://doi.org/10.3382/ps.0721301
  6. de-Deckere, E. A. M., van Amelsvoort, J. M. M., McNeill, G. P. and Jones, P. 1999. Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. Brit. J. Nutr. 82: 309-317.
  7. Dormandy, T. L. and Wickens, D. G. 1987. The experimental and clinical pathology of diene conjugation. Chem. Phys. Lipids. 45:353-364. https://doi.org/10.1016/0009-3084(87)90072-7
  8. Du, M., Ahn, D. U., Nam, K. C. and Sell, J. L. 2000. Influence of dietary conjugated linoleic acid on volatile profiles, color and lipid oxidation of irradiated raw chicken meat. Meat Sci. 56:387-395. https://doi.org/10.1016/S0309-1740(00)00067-X
  9. Dugan, M. E. R., Aalhue, J. L., Jeremiah, I. E., Kramer, J. K. G. and Schaefer, A. L. 1999. The effects of feeding conjugated linoleic acid on subsequent pork quality. Can. J. Anim. Sci. 79:45-51. https://doi.org/10.4141/A98-070
  10. Eggert, J. M., Belury, M. A., Kempa-Steczko, A. Mills, S. E. and Schinckel, A. 2001. Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs. J. Anim. Sci. 79(11):2866-2872.
  11. Eikelenboom, G., Hoving-Bolink, A. H., van der Wal, P. G., de Vries, A. W. and Vonder, G. 1992. De invloed van de eind-pH op de eetkwaliteit van varkensvlees. IVO-DLO Rapport B-385. Zeist. The Netherlands.
  12. Feldhusen, F. and Kuhne, M. 1992. Effects of ultra rapid chilling and ageing on length of sarcomeres and tenderness of pork. Meat Sci. 32:161-171.
  13. Fogerty, A. C., Ford, G. L. and Svoronos, D. 1988. Octadeca-9, 11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutrition Reports International 38:937-944.
  14. Folch, J., Lees, M. and Sloan-Stanley, G. H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Bio. Chem. 226:497-509.
  15. Fulghum, J. G., Sebranek, J. G., Jr. Parrish, F. C. and Wiegand, B. R. 1999. Effects of feeding conjugated linoleic acid to market pigs on bacon quality and composition. 1998. Swine Res. Rep., Iowa State Univ., Ames. pp. 231-232.
  16. Ha, Y. L., Grimm, N. K. and Pariza, M. W. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis. 8: 1881-1887.
  17. Ha, Y. L., Ha, H. S., Bahn, K. N., Lee, E. J. and Ha, J. K. 1994. Effects of dietary conjugated dienoic isomers of linoleic acid (CLA) on cholesterol and CLA contents of hen's egg. In 1994 IFT Annual Meeting Technical Program: Jun. 25-29. Altant, GA. USA. 79D 6, p249.
  18. Ha, Y. L., Storkson, J. and Pariza, M. W. 1990. Inhibition of benzo[$\alpha$]pyrene induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50:1097-1101.
  19. Haumann, B. F. 1996. Conjugated linoleci acid offers research promise. Inform. 7(2):152-159.
  20. Houseknecht, K. L., Vanden Heuvel, J. P., Moya-Camarena, S. Y., Portocarrero, C. P., Peck, L. W., Nickel, K. P. and Belury, M. A. 1998. Dietary conjugated linoleic acid normalizes impaires glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem. Biophys. Res. Comm. 244:678-682. https://doi.org/10.1006/bbrc.1998.8303
  21. Hughes, P. E., Hunter, W. J. and Tove, S. B. 1982. Biohydrogenation of unsaturated fatty acids. Purification and properties of cis,9-transoctadeca-dienoate reductase. J. Biol. Chem. 257, 3643-3649.
  22. Karmer, J. K. G., Sehat, N., Dugan, M. E. R., Mossoba, M. M., Yurawecz, M. A. and Ku, Y. 1998. Distribution of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by GC and silver ion-HPLC. Lipids. 33:549-558.
  23. Kim, C. J., Lee, E. S., Joo, S. T., Kim, B. C., Kang, J. O., Kauffman, R. G., Yoo, I. J., Ko, W. S. and Choi, D. Y. 1996. Chemical, physical and structural characteristics of pork loins from four quality groups. 42nd international congress meat science technology, Lillehammer, Norway.
  24. Koohmaraie, M., Killefer, J., Bishop, M. D., Shackelford, S. D., Wheeler, T. L. and Arbona, J. P. 1995. Calpastatin-based methods for predicting meat tenderness. In: Ouall, A., Demeyer, D. I., Smulders, F. J. M. (Eds). Expression of tissue proteinases and regulation of protein degradation as related to meat quality. ECCEAMST. Ⅲ. Utrecht. The Netherlands. pp. 395-412.
  25. Kritchevsky, D., Tepper, S. A., Wright, S., Tso, P. and Czarnecki, S. K. 2000. Influence of conjugated linoleic acid (CLA) on establishment and progression of atherosclerosis in rabbits. J. Ame. Colle. Nutr. 19: 472S-477S.
  26. Lee, K. N., Kritchevsky, D. and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 108:19-25. https://doi.org/10.1016/0021-9150(94)90034-5
  27. Lee, K. S. 1996. Conjugated linoleic acid and lipid metabolism. Ph.D. Thesis at the University of Wisconsin-Madison. p. 77-120.
  28. Miller, C. C., Park, Y., Pariza, M. W. and Cook, M. E. 1994. Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochem. Biophys. Res. Commun. 198:1107-1112.
  29. Mossoba, M. M., McDonald, R. E. and Armstrong, D. J. 1991. Identification of minor c18 triene and conjugated diene isomers in hydrogenated soybean oil and margarine by GC-MI-FT-IR spectroscopy. J. Chromatogr. Sci. 29:324-330. https://doi.org/10.1093/chromsci/29.8.324
  30. Nicolosi, R. J., Courtemanche, K. V., Laitinen, L., Scimeca, J. A. and Huth, P. J. 1993. Effect of feeding diets enriched in conjugated linoleic acid on lipoproteins and aortic atherogenesis in hamsters. Circulation 88 suppl. 2458.
  31. Nicolosi, R. J., Rogers, E. J., Kritchevsky, D., Scimeca, J. A. and Huth, P. J. 1997. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 22:266-277.
  32. Pariza, M. W. 1997. Conjugated linoleic acid a newly recognised nutrient. Chem. Ind. (London) No. 12, 464-466.
  33. Park, Y. H., Albright, K. j., Liu, W., Storkson, J. M., Cook, M. E. and Pariza, M. W. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853-858. https://doi.org/10.1007/s11745-997-0109-x
  34. Pettigew, J. E. and Esnaola, M. A. 2001. Swine nutrition and pork quality: A review. J. Anim. Sci. 79(E. Suppl.):E316-E342.
  35. SAS. 1999. SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary, NC, U.S.A.
  36. Shantha, N. C., Crum, A. D. and Decker, E. A. 1994. Evaluation of conjugated linoleic acid concentrations in cooked beef. J. Agric. Food. Chem. 42:1757-1760. https://doi.org/10.1021/jf00044a035
  37. Stangl, G. I. 2000. High dietary levels of a conjugated linoleic acid mixture alter hepatic glycerophospholipid class profile and cholesterol-carrying serum lipoproteins of rats. J. Nutr. Bioche. 11: 184-191. https://doi.org/10.1016/S0955-2863(00)00063-2
  38. Taylor, A. A., Nute, G. R. and Warkup, C. C. 1995. The effect of chilling, electrical stimulation and conditioning on pork eating quality. Meat Sci. 39:339-347. https://doi.org/10.1016/0309-1740(95)90392-M
  39. Thiel, R. L., Sparks, J. C., Wiegand, B. R., Jr. Parrish, F. C. and Ewan, R. C. 1998. Conjugated linoleic acid improves performance and body composition in swine. J. Anim. Sci. 76(Suppl. 2):57(abstract).
  40. Tornberg, E., von Seth, G. and Goransson, A. 1994. Influence of ageing time, storage temperature and percentage lean on the eating quality of pork and its relationship to instrumental and structural parameters. Sciences des Aliments. 14: 373-385.
  41. Yurawecz, M. P., Hood, J. K., Mossoba, M. M., Roach, J. A. and Ku, Y. 1995. Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid. Lipids. 30:595-598.
  42. 박병성. 1997. 새로운 축산식품 생산을 위한 지질 신소재. 한축축산식품학회지. 17: 118-130.
  43. 이정일, 주선태, 박태선, 신택순, 하영래, 박구부. 1999. Conjugated Linoleic Acid (CLA)가 축적된 계육의 저장기간중 이화학적 특성 변화. 한국축산식품학회지. 19(1):88-99.
  44. 이정일, 최진성, 박종대, 박준철, 김영화, 문홍길, 주선태, 박구부. 2001. Conjugated linoleic acid(CLA) 급여가 돈육 품질에 미치는 효과. 동물자원과학회지. 43(5):735-746.