Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Cell Cycle Arrest of Human Lung Carcinoma A549 Cells by an Aqueous Extract from the Roots of Platycodon grandiflorum

길경 수용액 추출물에 의한 인체 폐암세포의 성장억제 기전 연구

  • Kang Rak Won (Department of Internal Medicine, Oriental Medicine, Dongeui University) ;
  • Lee Jae Hun (Biochemistry, Oriental Medicine, Dongeui University) ;
  • Kam Cheol Woo (Department of Internal Medicine, Oriental Medicine, Dongeui University) ;
  • Choi Byung Tae (Anatomy, College of Oriental Medicine and Research Institute of Oriental Medicine, Dongeui University) ;
  • Choi Yung Hyun (Biochemistry, Oriental Medicine, Dongeui University) ;
  • Park Dong Il (Department of Internal Medicine, Oriental Medicine, Dongeui University)
  • 강락원 (동의대학교 한의과대학 내과학교실) ;
  • 이재원 (동의대학교 한의과대학 생화학교실) ;
  • 감철우 (동의대학교 한의과대학 내과학교실) ;
  • 최병태 (동의대학교 한의과대학 해부학교실 및 한의학연구소) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 박동일 (동의대학교 한의과대학 내과학교실)
  • Published : 2003.02.01
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Abstract

Platycodi Radix, the root of Platycodon grandiflorum, commonly known as Doraji, is used as a traditional oriental medicine. Extracts from the roots of P. grandiflorum have been reported to have wide ranging health benefits. We investigated the effects of an aqueous extract from the roots of P. grandiflorum (AEPG) on the cell proliferation of human lung carcinoma A549 cells in order to understand its anti-proliferative mechanism. AEPG treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. This anti-proliferative effect of A549 cells by AEPG treatment was associated with morphological changes such as membrane shrinking, cell rounding up and inhibition of cell migration. DNA flow cytometric histograms showed that populations of both Sand G2/M phase of the cell cycle were increased by AEPG treatment in a concentration-dependent manner. AEPG treatment induced a marked accumulation of tumor suppressor p53 and a concomitant induction of cyclin-dependent kinase (Cdk) inhibitor p21 and p27. In addition, SSS treatment resulted in down-regulation of Cdk2 and Cdk4 expression. The present results indicated that AEPG-induced inhibition of lung cancer cell proliferation is associated with the blockage of S to G2/M phase progression the induction of apoptosis. Taken together, these findings suggest that P. grandiflorum has strong potential for development as an agent for prevention against human lung cancer.

Keywords

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