Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Determination of Volatile Fatty Acids in Aqueous Samples by HS-SPME with In-Fiber Derivatization

Fiber내 유도체화/HS-SPME를 이용한 수용액 시료 중 휘발성 지방산의 분석

  • Ahn, Yun Gyong (Hazardous substance research team, Korea Basic Science Institute) ;
  • Lee, Jee Yeon (Hazardous substance research team, Korea Basic Science Institute) ;
  • Kim, Jeehyeong (Department of Civil Environment Engineering, Korea University) ;
  • Hong, Jongki (Hazardous substance research team, Korea Basic Science Institute)
  • 안윤경 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 이지연 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 김지형 (고려대학교 토목환경공학과) ;
  • 홍종기 (한국기초과학지원연구원 유해물질분석연구팀)
  • Received : 2003.10.08
  • Accepted : 2003.11.10
  • Published : 2003.12.25
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Abstract

The HS (headspace)-SPME (Solid phase microextraction) as rapid and simple method was performed for the determination of volatile fatty acids (VFAs) from the aqueous samples. In-fiber derivatization of VFAs with 1-Pyrenyldiazomethane (PDAM) was applied to improve their sensitivity of detection. In SPME procedure, typical parameters such as effects of solution pH, and salting out reagent and ultrasonication were investigated to improve the extraction efficiency. Based on the developed method, VFAs in wastewater samples were determined by gas chromatography / mass spectrometry-selected ion monitoring (GC/MS-SIM) mode.

수용액 시료 중 휘발성 지방산을 HS (headspace)-SPME (Solid phase microextraction)를 이용하여 간편하고 빠르게 분석하는 방법에 대하여 연구하였다. 1-Pyrenyldiazomethane (PDAM)을 이용한 화이버 내 유도체화를 통하여 휘발성 지방산의 검출감도를 향상시킬 수 있었으며, SPME 추출조건으로 pH, 염 효과 및 초음파 추출에 대하여 조사하였다. 본 연구에서 개발된 방법을 기반으로 실제 폐수 중 휘발성 지방산을 추출하여 기체크로마토그래피/질량분석기-선택이온검색법으로 정량 분석하였다.

Keywords

References

  1. A. Tangerman, F. M. Nagengast, Anal. Biochem., 236, 1-8(1996)
  2. G. Manni, F. Caron, J. Chromatogr A., 690, 237-242(1995)
  3. Z. H. Huang, C. C. Sweeley, Anal. Chim Acta, 259, 185-192(1992)
  4. D. W. Rhodes, Soil Sci. Am. Proc., 21, 389-392(1957)
  5. P. D. Ahlfeld, M. M. John and F. P. George, Water Res. 9, 77-84(1986)
  6. M. C. Wentzel, G. A. Ekama and G. Marais, Water Sci. Technol., 23, 555-568(1995)
  7. A. E. Greenberg, R. R. Trussell and L. S. Clesceri, 'Standard Methods for the Examination of Water and Wastewater', 16th Ed., p. 504, American Public Health Association, Washington, DC, U.S.A., 1985
  8. T. J. Clark, J. E. Bunch, J. Chromatogr. Sci., 35, 206-219(1997)
  9. A. Vairamurthy, K. Mopper, Anal. Chim Acta, 237, 215-221(1990)
  10. M. Abalos, J. M. Bayona and J. Pawliszyn, J. Chromatogr A, 873, 107-115(2000)
  11. M. Abalos, J. M. Bayona, J. Chromatogr A., 891, 287-294(2000) https://doi.org/10.1016/S0021-9673(00)00655-5
  12. I. Murad, H. Helalh, S. Fujii and T. Korenaga, Talanta, 54, 1039-1047(2001) https://doi.org/10.1016/S0039-9140(01)00386-1
  13. G. E Bailescu, V. A. Ilie, ' Stationary Phases in Gas Chromatography', B. M. Trost, J. Fleming, Ed., Pergamon Press, Oxford, UK, 1975
  14. I. Murad, H. Helalh, Y. Takabayashi, S. Fujii and T. Korenaga, Anal. Chim Acta, 428, 227-234(2001) https://doi.org/10.1016/S0003-2670(00)01251-4
  15. J. G. Andrew, Groen L. A. and M. E. Johnson, J. Chromatogr A., 849, 541-552 (1999)
  16. G. Gmeiner, P. Gartner, C. Krassnig and H. Tausch, J. Chromatogr B., 766, 209-218(2002)
  17. L. Pan and J. Pawliszyn, Anal. Chem., 69(2), 196-205(1997) https://doi.org/10.1021/ac9606362
  18. S. H. Pine, 'Organic Chemistry,' 5th Ed., p. 272, McGraw-Hill, New York, U. S. A., 1985
  19. Wang, Y. Ph. D. Thesis : A study of solid phase microextraction and headspace sampling., University of virginia, 1997.
  20. Y. Jianzhen, C. F. Richard and H. S. John, Environ. Sci. Techno!' 32(16), 2357-2370 (1998)
  21. J. Schneede, P. M. Ueland, Ana!' Chern., 64, 315-319(1992)
  22. O. Pinho, I. M. Ferreira and M. A. Ferreira, Anal. Chern., 74(20), 5199-5204(2002)
  23. J. A. Cruwys, R. M. Dinsdale, F. R. Hawkes and D. L. Hawkes, J. Chromatogr A., 945, 195-209(2002)
  24. L. Pan, M. Adams and J. Pawliszyn, 67(23), Anal. Chem., 4396-4403(1995)
  25. T. Lewis, P. D. Nichols and T. A. McMeekin, Microbiological Methods, 43, 107-116(2000)
  26. M. Mecozzi, M. Amici, G. Romanelli, E. Pietrantonio, and A. Deluca, J.Chromarogr A., 963, 363-373(2002)