Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Genetic Identification of the North Pacific Chum Salmon (Oncorhynchus keta) Stocks

유전적 형질에 의한 북태평양 연어 (Oncorhynchus keta)의 계군 구분

  • JUNG Woongsic (Polar Sciences Laboratory Korea Ocean Research &, Development Institute) ;
  • LEE Youn-Ho (Polar Sciences Laboratory Korea Ocean Research &, Development Institute) ;
  • KIM Suam (Department of Marine Biology, Pukyong National University) ;
  • JIN Deuk-Hee (Faculty of Marine Bioscience & Technology, Kangnung National University) ;
  • SEONG Ki Baek (Yang Yang Inland Fisheries Research Institute)
  • Published : 2003.12.01
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Abstract

The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.

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References

  1. Allendorf, F.W. and L.W. Seeb. 2000. Concordance of genetic divergence among sockeye salmon populations at allozyme, nuclear DNA, and mitochondrial DNA markers. Evolution, 54, 640-651 https://doi.org/10.1111/j.0014-3820.2000.tb00065.x
  2. Avise, J.C. 1994. Molecular Markers, Natural History and Evolution. New York
  3. Beacham, T.D., S. Pollard and K.D. Le. 2000. Microsatellite DNA population structure and stock identification of steelhead trout (Oncorhynchus mykiss) in the Nass and Skeena rivers in northern British Columbia. Mar. Biotechnol., 2, 587-600 https://doi.org/10.1007/s101260000045
  4. Brown, W.M., M. George, Jr. and A.C. Wilson. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci. USA, 76, 1967-1971 https://doi.org/10.1073/pnas.76.4.1967
  5. Choi, Y.S. 2002. Cloning and nucleotide sequence of mitochondrial NADH dehydrogenase subunit 3 DNA for population analysis of chum salmon,Oncorhynchus keta. Master's thesis, Gangnung Univ. (in Korean)
  6. Choi, Y.S., Y.H. Lee and D.H. Jin. 2003. Cloning and DNA sequences analysis of mitochondrial NADH dehydrogenase subunit 3 from Korean chum salmon, Oncorhynchus keta. J. Kor. Fish. Soc. (in press)
  7. Clement, M., D. Posada and C.A. Crandall. 2000. TCS: a computer program to estimate gene genealogies. Mol. Ecol., 9, 1657-1660 https://doi.org/10.1046/j.1365-294x.2000.01020.x
  8. Cornuet, J.M. and G. Luikart. 1996. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics, 144, 2001-2014
  9. Domanico, M.J. and R.B. Phillips. 1995. Phylogenetic analysis of Pacific salmon (Genus Oncorhynchus) based on mitochondrial DNA sequence data. Mol. Phylogenet. Evol., 4, 366-371 https://doi.org/10.1006/mpev.1995.1034
  10. Domanico, M.J., R.B. Phillips and T.H. Oakley. 1997. Phylogenetic analysis of Pacific salmon (genus Oncorhynchus) using nuclear and mitochondrial DNA sequences. Can. J. Fish. Aquat. Sci, 54, 1865-1872 https://doi.org/10.1139/cjfas-54-8-1865
  11. Hong K..P., J.G. Myoung, J.K. Son and C.W. Park. 1994. A biochemical study for the development of genetic marker on salmonids in Korea. Bull. K-or. Fish. Soc.,27, 83-88
  12. Jacobs, H.T., D.J. Elliott, V.B. Math and A. Farquharson. 1988. Nucleotide sequence and gene organization of sea urchin mitochondrial DNA. J. Mol. Biol., 202, 185-217 https://doi.org/10.1016/0022-2836(88)90452-4
  13. Jarne, P. and J.L. Lagoda. 1996. TREE., 11, 424-429
  14. Jung, W.S., Y.H. Lee, H.C. Shin, K.B. Seong and S.A. Kim. 2001. Species identification of the chum salmon (Oncorhynchus keta) and pink salmon (0. gorbuscha) by use of genetic markers. J. Kor. Soc. Fish. Res., 4, 30-41. (in Korean)
  15. Kitano, T., N. Matsuoka and N. Saitou. 1997. Phylogenetic relationship of the genus Oncorhynchus species inferred from nuclear and mitochondrial markers. Genes Genetic Syst., 72, 25-34 https://doi.org/10.1266/ggs.72.25
  16. Lee, H.J., J.Y. Park, J.H. Lee, K.S. Min, I.G. Jeon, M.A. Yoo and W.H. Lee. 2000. Phylogeny of the subfamily Salmonidae distributed in Korea based upon nu cleotide sequences of mitochondrial ribosomal RNA genes. J. Kor. Fish. Soc., 33, 103-109
  17. McConnell, S., L. Hamilton, D. Morris, D. Cook, D. Paquet, P. Bentzen and J. Wright. 1995. Isolation of salmonid microsatellite loci and their application to the population genetics of Canadian east coast stocks of Atlantic salmon. Aquaculture, 137, 19-30 https://doi.org/10.1016/0044-8486(95)01111-0
  18. McKay, S.J., R.H. Devlin and M.J. Smith. 1996. Phylogeny of Pacific salmon and trout based on growth hormone type-2 and mitochondrial NADH dehydrogenase subunit 3 DNA sequences. Can. J. Fish. Aquat. Sci., 53, 1165-1176 https://doi.org/10.1139/cjfas-53-5-1165
  19. McPhail, J.D. and C.C. Lindsey. 1970. Freshwater fishes of northwestern Canada and Alaska. Bull. Fish. Res. Board Can., 173, 381
  20. McPhail, J.D. and C.C. Lindsey. 1986. Zoogeography of the freshwater fishes of Cascadia (the Columbia system and rivers north to the Stikine). In: Zoo-geography of North American Freshwater Fishes, Hocutt, C.H. and E.O. Wiley, ed. Wiley and Sons, New York, N.Y., PP. 615-637
  21. Myoung, J.G. and C.W. Park. 1992. Morphological and taxonomic studies of Oncorhynchus sp. in Korea. Korea Ocean Research and Development Institute. BSPE 00254-420-3. (in Korean)
  22. Nei, M., T. Maruyama and R. Chakraborty. 1975. The bottleneck effect and genetic variability in populations. Evolution, 29, 1-10 https://doi.org/10.2307/2407137
  23. Nelson, R.J., T.D. Beacham and M.P. Small. 1998. Microsatellite analysis of the population structure of a Vancouver Island sockeye salmon (Oncorhynchus nerkd) stock complex using nondenaturing gel electrophoresis. Mol. Mar. Biol. Biotechnol., 7, 312-319
  24. O'Connell, M., D.O.F. Skibinski and J.A. Beardmore. 1996. Allozyme and mtDNA divergence between Atlantic salmon populations in North Wales. J. Fish Biol., 48, 1023-1026 https://doi.org/10.1111/j.1095-8649.1996.tb01498.x
  25. Olsen, J.B., P. Bentzen and J.E. Seeb. 1998. Characterization of seven microsatellite loci derived from pink salmon. Mol. Ecol., 7, 1083-1090 https://doi.org/10.1046/j.1365-294X.1998.00401.x
  26. Seong, K.B. 1999. Biological characteristics and population genetics of salmonid species from Korea. Ph. D. Thesis, Pukyong Nat'l Univ., PP. 125. (in Korean)
  27. Sunnucks, P. 2000. Efficient genetic markers for population biology. TREE., 15, 199-203
  28. Taylor, E.B., T.D. Beacham and M. Kaeriyama. 1994. Population structure and identification of north Pacific ocean chum salmon (Oncorhynchus keta) revealed by an analysis of minisatellite DNA variation. Can. J. Fish. Aquat, Sci, 51, 1430-1442 https://doi.org/10.1139/f94-143
  29. Utter, F.M., S.G. Campton, G. Miller, J. Seeb and L. Wishard. 1980. Population structure of indigenous salmonid species of the Pacific Northwest. In: Salmonid ecosystems of the North Pacific, McNeil, W.J. and D.C. Himsworth, ed. Oregon State Univ. Press, PP. 285-304
  30. Wright, J.M. and P. Bentzen. 1994. Microsatellites: genetic markers for the future. Fish Biol. Fish., 4, 384-388 https://doi.org/10.1007/BF00042912

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