Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Cloning of Melanin Concentrating Hormone cDNA Gene from Olive Flounder (Paralichthys olivaceus)

넙치 (Paralichthys olivaceus)에서 멜라닌 농축 호르몬 cDHA 유전자의 클로닝

  • JEON Jeong Min (Department of Microbiology, Pukyong National University) ;
  • SONG Young Hwan (Department of Microbiology, Pukyong National University)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Melanin concentrating hormone (MCH) regulating color change of fish skin was identified from brain cDNA library of Olive flounder (Paralichthys olivaceus) during the analysis of Expressed Sequence Tags (ESTs). Olive flounder MCH gene consisted of 598 nucleotides encoding 150 amino acids. Olive flounder MCH protein revealed to contain signal peptide of 19 amino acid residues, pro-MCH of 131 amino acids being processed to biologically active and mature form of hormone with 25 amino acid residues at the carboxyl terminus. A comparative structural analysis revealed that Olive flounder MCH precursor had low sequence identity with other fish species and mammalian counterparts, while the amino acid sequences of mature hormone had a relatively high identity and more conserved. RT-PCR analysis revealed that olive flounder MCH precersor gene was expressed spectically only in the brain and not in other tissues.

Keywords

References

  1. Baker, B.I., D.J. Bird and J.C. Buckingham. 1985. Salmonid melanin-concentrating hormone inhibits corticotrophin release. J. Endocrinol., 106, R5-R8 https://doi.org/10.1677/joe.0.106R005
  2. Baker, B.I., D.J. Bird and J.C. Buckingham. 1986. Effects of chronic administration of melanin-concentrating hormone on corticotrophin, melanotrophin, and pigmentation in the trout. Gen. Comp. Endocrinol., 63,62-69 https://doi.org/10.1016/0016-6480(86)90182-6
  3. Baker, B., A. Levy, L. Hall and S. Lightman. 1995. Cloning and expression of melanin-concentrating hormone genes in the rainbow trout brain. Neuroendocrinology, 61, 67-76 https://doi.org/10.1159/000126814
  4. Breton, C., F. Presse, G. Hervieu and J. Nahon. 1993. Structure and regulation of the mouse melaninconcentrating hormone mRNA and gene. Mol. Cell. Neurosci., 4, 271-284 https://doi.org/10.1016/0306-4522(79)90088-5
  5. Cai, C.X., D.E. Birk and T.F. Linsenmayer. 1997. Ferritin is a developmentally regulated nuclear protein of avian corneal epithelial cells. J. BioI. Chem., 272, 12831-12839 https://doi.org/10.1074/jbc.272.19.12831
  6. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem., 162, 156-159
  7. Enami, M. 1955. Melanophre-contracting hormone (MCH) of possible hypothalamic origin in the catfish, Parasilurus. Science, 121, 36-37 https://doi.org/10.1126/science.121.3132.36
  8. Francis, K., M. Suzuki and B.I. Baker. 1997. Responses of melanin-concentrating hormone mRNA to salt water challenge in the rainbow trout. Neuroendocrinology, 66, 195-202 https://doi.org/10.1159/000127238
  9. Griffond, B. and B.I. Baker. 2002. Cell and Molecular cell biology of melanin-concentrating hormone. Internat. Rev. Cytol., 213, 233-277 https://doi.org/10.1016/S0074-7696(02)13016-6
  10. Groneveld, D., M.J. Hut, P.H.M. Balm, G.J.M. Martens and S.E. Wendelaar Bonga. 1993. Cloning and sequence analysis of hypothalamus cDNA encoding tilapia melanin-concentrating hormone. Fish Physiol.Biochem., 11, 117-124 https://doi.org/10.1007/BF00004557
  11. Harris, L. and D.J. Bird. 1998. Alpha-melanocyte stimulating hormone (a-MSH) and melanin-concentrating hormone (MCH) stimulate phagocytosis by head kidney leucocytes of rainbow trout (Oncorhynchus mykiss) in vitro. Fish Shellfish Immunol., 8, 631-638 https://doi.org/10.1006/fsim.1998.0172
  12. Kawauchi, H., I. Kawazoe, M. Tsubokawa, M. Kishida and B.I. Baker. 1983. Characterization of melaninconcentrating hormone in chum salmon pituitaries.Nature, 305, 321-323 https://doi.org/10.1038/305321a0
  13. Matsunaga, T.O., A.M. Castrucci, M.E. Hadley and V.J. Hruby. 1989. Melanin concentrating hormone (MCH):synthesis and bioactivity studies of MCH fragment analogues. Peptides, 10, 349-354 https://doi.org/10.1016/0196-9781(89)90042-9
  14. Minth, C.D., H. Qiu, H. Akil, S.J. Watson and J.E. Dixon.1989. Two precursors of melanin-concentrating hormone: DNA sequence analysis and in situ immunochemical localization. Proc. Nat'l. Acad. Sci. USA, 86, 4292-4296 https://doi.org/10.1073/pnas.86.11.4292
  15. Nahon, J.L., F. Presse, J.C. Bittencourt, P.E. Sawchenko and W. Vale. 1989. The rat melanin-concentrating hormone messenger ribonucleic acid encodes multiple putative neuropeptides coexpressed in the dorsolateral hypothalamus. Endocrinology, 125, 2056-2065 https://doi.org/10.1210/endo-125-4-2056
  16. Nahon, J.L., F. Presse, R. Schoepfer and W. Vale. 1991. Identification of a single melanin-concentrating hormone messenger ribonucleic acid in coho salmon: structural relatedness with ribonucleic acid. J. Neuroendocrinol. 3,173-183 https://doi.org/10.1111/j.1365-2826.1991.tb00260.x
  17. Nielsen, H., J. Engelbrecht, S. Brunak and G. Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage site.Protein Eng., 10, 1-6 https://doi.org/10.1093/protein/10.1.1
  18. Ono, M., C. Wada, I. Oikawa, I. Kawazoe and H.Kawauchi. 1988. Structures of two kinds of mRNA encoding the chum salmon melanin-concentrating hormone. Gene, 71, 433-438 https://doi.org/10.1016/0378-1119(88)90060-1
  19. Presse, F., J.L. Nahon, W.H. Fischer and W. Vale. 1990. Structure of the human melanin concentrating hormone mRNA. Mol. Endocrinol., 4, 632-637 https://doi.org/10.1210/mend-4-4-632
  20. Saitou, N. and M. Nei. 1987. The Neighbor-joining Method: A new method for reconstructing phylogenetic trees. Mol. BioI. Evol., 4, 406-425
  21. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, pp. 545
  22. Toumaniantz, G., P.C. Rerreira, I. Allaeys, J.C. Bittencourt and J.L. Nahon. 2000. Differential neuronal expression and projections of melanin-concentrating hormone (MCH) and MCH gene-overprinted polypeptide (MGOP) in the rat brain. Eur. J. Neurosci., 12, 4367-4380 https://doi.org/10.1046/j.0953-816X.2000.01340.x
  23. Wilkes, B.C., V.J. Hruby, A.M. Castrucci, W.C. Sherbrooke and M.E. Hadley. 1984. Synthesis of a cyclic melanotropic peptide exhibiting both melanin-con centrating and -dispersing activities. Science, 224, 1111-1113 https://doi.org/10.1126/science.6609433