Journal of Acupuncture Research

  • 제20권4호
  • /
  • Pages.53-65
  • /
  • 2003
  • /
  • 2586-288X(pISSN)
  • /
  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

봉약침액(蜂藥鍼液)의 NO 소거 및 Chemokine 유전자 발현에 대한 효과

Nitric Oxide Scavenging Effect and Expression of Chemokine Genes in Bee Venom

  • 조태성 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실)
  • Cho, Tae-sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Youn, Hyoun-min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Ahn, Chang-beohm (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University)
  • 투고 : 2003.06.26
  • 심사 : 2003.07.12
  • 발행 : 2003.08.20
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초록

Although the effect of Bee Venom has been reported, its mechanism has not been fully elucidated. Nitric Oxide(NO) is one of the free radicals and mediates in inflammation diseases. Chemokines contribute to the pathogenesis of several disorders such as allergic rhinitis and rheumatoid arthritis and so on. The objective of this study was to investigate the scavenging effect of Bee Venom on NO and on expression of chemokine genes. There was no significant NO scavenging effect in Crude Bee Venom, Apamin, Melittin, and MCD-peptide. The expression of chemokines was examined by RT-PCR using the human mast cell line(HMC-1), which is known to secrete and express chemokines. In order to investigate the protective effect of Bee Venom, HMC-1 cells were incubated with pretreatment of Bee Venom for 24 hrs and stimulated with 1 uM calcium ionophore A23178 for 2 hrs. RT-PCR analyses of chemokine genes showed that expressions of RANTES and MCP-1 were increased compared to the calcium ionophore-only treated group. But IL-8 and MCP-3 did not express increasing effect compared to control group. This study may provide important basic data on the possibility of the clinical treatment of Bee Venom in inflammation diseases.

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