Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Gene Expression Analysis Using cDNA Microarray Assay by Cervi Pantotricuhum Cornu Herbal Acupuncture

녹용약침액(鹿茸藥鍼液)의 DNA chip을 이용(利用)한 유전자(遺傳子) 발현(發顯) 분석(分析)

  • Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Lee, Yun-ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Choi, Je-yong (Department of Biochemistry, College of Medicine, Kyung-Pook University)
  • 한상원 (경산대학교 한의과대학 침구학교실) ;
  • 서정철 (경산대학교 한의과대학 침구학교실) ;
  • 이윤호 (경희대학교 한의과대학 침구학교실) ;
  • 최제용 (경북대학교 의과대학 생화학교실)
  • Received : 2003.03.25
  • Accepted : 2003.05.17
  • Published : 2003.06.20
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Abstract

Objective : Bone homeostasis is maintained by balance of bone formation and resorption. Therefore, bone related diseases arose by disturbance of this balance between osteoblast and osteoclast activities. To develop a successful screening system the therapeutic components based on oriental medicine is essential to set up systematic approach for that purpose. The purpose of this study is to the know the gene expression using cDNA microarray assay. Methods : Cervi Pantotricuhum Cornu Herbal-acupuncture extract was prepared by boiling. human osteosarcoma cells(HOS) were treated with Cervi Pantotricuhum Cornu Herbal-acupuncture solution. Then mRNA was extracted and cDNA microarray assay was performed. Results : Human osteosarcoma cells(HOS) treated with Cervi Pantotricuhum Cornu Herbal-acupuncture solution($500{\mu}g/m{\ell}$) showed that thioredoxin, TAFII31 and two novel genes were increased. However many genes decreased their expression by Cervi Pantotricuhum Cornu Herbal-acupuncture. Conclusions : This type of approach will give a good chance to explore the favorable effects of Cervi Pantotricuhum Cornu Herbal-acupuncture. Further study is needed for investigating the effect of Cervi Pantotricuhum Cornu Herbal-acupuncture.

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