대한수의학회지 (Korean Journal of Veterinary Research)

  • 제43권1호
  • /
  • Pages.67-75
  • /
  • 2003
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

집단 개사육농장에서의 Canine Brucellosis 발생 및 PCR-RFLP를 이용한 분리주의 특성조사

Occurrence of canine brucellosis in large kennels and characterization of Brucella canis isolates by PCR-RFLP

  • Kim, Jong-Wan (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Young-Ju (College of Veterinary Medicine, Kyungpook National University) ;
  • Tak, Ryun-Bin (College of Veterinary Medicine, Kyungpook National University)
  • 심사 : 2003.03.05
  • 발행 : 2003.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A total of 260 dogs were randomly selected from two different treed kennels that brucellosis has occurred (group 1, 126 dogs), and random selected breed kennel (group 2, 134 dogs), and monitored for Brucella canis (B. canis) by 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and bacterial culture method. For the differentiation, PCR-RFLP using omp-31, wbkA and per genes used for 52 of B canis strains (strain I) isolated in this study and 3 of B. canis strains (strain II) isolated in 1994 in Korea. 2ME-RSAT revealed that 63/126 dogs (50.0%) and 12/134 dogs (9.0%) were positive in group I and group II, respectively. Bacterial culture revealed that 47/126 dogs (37.3%) and 5/134 dogs (3.7%) were positive in group I and group II, respectively. As the results of PCR-RFLP, $\underline{omp}-31$ was amplified from all Brucella spp, except B. abortus. All B. canis isolates showed unique PCR-RFLP pattern following digestion with Bmel8I. However, all Brucella spp. showed the same PCR-RFLP pattern following digestion with SalI. PCR-RFLP analysis of wbkA revealed that all Brucella spp. showed the same pattern following digestion with HindIII. PCR-RFLP analysis of per revealed that B. abortus 544 and B. melitensis 63/9 showed the same pattern, but different from B. suis and B. canis following digestion with HindIII.

키워드

참고문헌

  1. Alton GG, Jones LM, Pietz DE. 1975. Laboratory Techniques in Brucellosis. WHO. Geneva
  2. Carmichael LE, Kenney RM. Canine abortion caused by Brucella canis. J Am Vet Med Assoc, 152:605-616, 1968
  3. Shin S, Carmichael LE. 1999. Canine brucellosis caused by Brucella canis. Recent advances in canine infectious disease, http://www.ivis.org
  4. Tak RB, Chun DK. Isolation of Brucella suis from aborted fetus of a dog. J Kor Soc Microbiol, 7:17-20, 1972
  5. 박용호, 강승원, 주이석 등. 개부루세라 진단액생산 기초시험 및 항체조사. 가축위생연구소 시험보고, 94-98, 1984
  6. 문진산, 오기석, 박인철 등. 전남지방의 소형견 번식장으로부터 발생한 canine brucellosis. 대한수의학회지, 39:1099-1105, 1999
  7. 박청규, 오지연. 대구지역 개의 Brucella canis감염에 대한 세균학적 및 혈청학적 조사. 대한수의학회지, 41:67-71, 2001
  8. Baldi PC, Wanke MM, Loza ME, et al. Brucella abortus cytoplasmic protein used as antigens in ELISA potentially useful for the diagnosis of canine bnicellosis. Vet Microbiol, 41:127-134, 1994
  9. Baldi PC, Wanke MM, Loza ME, et at. Diagnosis of canine brucellosis by detection of serum antibodies against an 18 kDa cytoplasmic protein of Brucella spp. Vet MicrobioI, 51:273-281, 1997
  10. Antonio EM. Martin M, Soler M. Use of indirect enzyme-linked immunasorbent assay with hot saline solution extracts of a variant(M-) strain of Brucella canis for diagnosis of brucellosis in dogs. Am J Vet Res. 54:1043-1046, 1993
  11. Serikawa T, Iwaki S, Mori M, et al. Purification of a BrucelIa canis cell wall antigen by using immunosorbent columns and use of the antigen in enzyme-linked immnunosorbent assay for specific diagnosis of canine brucellosis. J Clin Microbiol, 27:837-842, 1989
  12. Johnson CA, Walker RD. Clinical signs and diagnosis of Brucella canis infection. The Compendium, 14:763-772, 1992
  13. Verger JM, Grimont F, Grimont PAD, et al. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int J Syst Bacteriol, 35:292-295, 1985
  14. Vizcaino N, Verger JM, Grayon M, et al. DNA polymoiphism at the omp-31 locus of Brucella spp. : evidence for a large deletion in Brucella abortus and other species-specific markers. MicrobioI, 143:2913-2921, 1997
  15. Qoeckaat A, Gtayon M, Vager JM, et al. Conservation of seven genes involved in the biosynthesis of the lipopolysaccharide O-side chain in Brucella spp. Res MicrobioI, 151:209.216, 2000
  16. Tchameva E, Rijpens N, Naydensky C, et al. Repedtive element sequence based polymerase chain reaction for typing of Brucella strains. Vet Microbiol, 51:169-178, 1996
  17. Mercier E. Jumas-Bilak E, Allardet-Servent A, et al. Polymorphism in Brucella strains detected by studying distribution of two short repetitive DNA elements. J Clin MicrobioI. 34: 1299-1302, 1996
  18. Tcharneva E, Rijpens N, Jersek B, et al. Differentiation of BruceIla species by Random Amplified Polymorphic DNA analysis. J Appl Microbiol, 88:69-80, 2000
  19. Vizcaino N, Cloeckaert A, Vergerb JM, et al. DNA polymorphism in the genus Brucella. Microbes Infect, 2:1089-1100, 2000
  20. Bricker BJ. PCR as a diagnostic tool for brucellosis. Vet MicrobioI, 90:435.446, 2002
  21. Sifuentes-Rincon AM, Revol A, Barrera-Saldana HA. Detection and differentiation of the six Brucella species by polymerase chain reaction. MoI Med, 3:734-739, 1997
  22. Gaviria-Ruiz MM, Cardona-Castro NM Evaluation and comparison of different blood culture techniques for bacteriological isolation of Salmonella typhi and Brucella abortus. J CIin Microbiol. 33:868-871, 1995
  23. Carmichael LE, Joubert JC A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant(M-) organism as antigen. Cor-nell Vet, 77:3-12, 1987
  24. 문지산, 박용호, 정석찬 둥. 개부루세라병의 혈청학적 진단법에 관한 연구. 농업과학논문집, 36:614-621, 1994
  25. Serikawa T, Muraguchi T, Nakao N. A survey of dogs gifu and shiga area for BruceIla canis. Jap. J Vet Sci, 39:635-642, 1977
  26. Shang, DQ. Investigation of B. canis infection in China. Zhonghw Liu Xine Bing Xue Za Zhi, 10:24-29, 1989
  27. Yagupsky P. MINIREVIEW. Detection of BruceIlae in blood cultures. J Clin Microbiol, 37:3437-3442, 1999