Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Development of multiplex polymerase chain reaction for the detection of vancomycin resistant genotypes and Enterococcus Sp.-specific genes

장구균의 vancomycin 내성 유전자와 종 특이유전자의 검출을 위한 Multiplex polymerase chain reaction 개발

  • Cho, Yun-Sang (National Veterinary Research and Quarantine Service) ;
  • Lee, Hee-Soo (National Veterinary Research and Quarantine Service) ;
  • Kim, Jong-Man (National Veterinary Research and Quarantine Service) ;
  • Ahn, Jong-Sam (National Veterinary Research and Quarantine Service) ;
  • Ryu, Pan-Dong (College of Veterinary Medicine, Seoul National University) ;
  • Park, Yong-Ho (College of Veterinary Medicine, Seoul National University) ;
  • Yoo, Han-Sang (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Mun-Han (College of Veterinary Medicine, Seoul National University)
  • Accepted : 2003.02.28
  • Published : 2003.03.31
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Abstract

A multiplex PCR assay, which allows simultaneous detection of vancomycin resistant genotypes and Enterococcus species-specific genes, was developed. Vancomycin resistant enterococci (VRE) from chickens and humans could be detected for vanA, vanB, vanC-1, vanC-2, $ddl_{E.faecium}$ and $ddl_{E.faecalis}$ by multiplex PCR. Eight isolates of VRE from humans (n=11) had $ddl_{E.faecium}$ and vanA, and 3 isolates of the VRE had $ddl_{E.faecium}$ and vanB. One isolate of VRE from chickens (n=6) had $ddl_{E.faecium}$ and vanA, and 5 isolates of the VRE had only vanA. E. faecium, E. faecalis, E. gallinarum and E. casseliflavus were also confirmed for the species-specific gene by multiplex PCR. This multiplex PCR could detect E. faecium, E. faecalis, E. gallinarum, E. casseliflavus, vanA, vanB, vanC-1 and vanC-2, simultaneously. The PCR assay established in the present study can be an alternative to time-consuming biochemical tests and antibiotic susceptibility tests of Enterococcus spp.

Keywords

References

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