Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Nonadrenergic Noncholinergic Nerve-mediated Contraction of the Longitudinal Muscle of Rat Ileum

랫드 회장 종주근의 비아드레날린 비콜린성 신경에 의한 수축반응

  • Kim, Tae-wan (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • La, Jun-ho (College of Veterinary Medicine, Seoul National University) ;
  • Sung, Tae-sik (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Jung-woo (College of Veterinary Medicine, Seoul National University) ;
  • Yang, Il-suk (College of Veterinary Medicine, Seoul National University) ;
  • Han, Ho-jae (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University)
  • 김태완 (전남대학교 수의과대학 생리학교실/생물공학연구소) ;
  • 나준호 (서울대학교 수의과대학 생리학교실) ;
  • 성태식 (서울대학교 수의과대학 생리학교실) ;
  • 강정우 (서울대학교 수의과대학 생리학교실) ;
  • 양일석 (서울대학교 수의과대학 생리학교실) ;
  • 한호재 (전남대학교 수의과대학 생리학교실/생물공학연구소)
  • Accepted : 2003.08.07
  • Published : 2003.09.25
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Abstract

The purpose of this study was to assess the role of tachykinins (TK) in mediating nonadrenergic noncholinergic (NANC) contractions produced by electrical field stimulation (EFS) in the longitudinal muscle of the rat ileum. In the presence of atropine ($1{\mu}M$), guanethidine ($5{\mu}M$), and L-nitroarginine (L-NNA, $200{\mu}M$), EFS (0.5ms pulse duration, 120 V, 1-20 Hz for 2 min) produced a frequency-dependent slowly-developing tonic contraction with superimposed phasic contractions ('on'-contraction) followed by off slowly-decreasing tonic and superimposed phasic contractions ('off'-contraction) of mucosa-free longitudinal oriented muscle strip. These EFS induced responses were blocked by tetrotoxin. $NK_1$ receptor selective antagonist L-732,138 strongly inhibited the EFS-induced excitatory responses. However $NK_2$ receptor selective antagonist, GR 159897 and $NK_3$ receptor selective antagonist SB 222200 did not significantly inhibited the responses. $NK_1$ receptor selective agonist [$Sar^9$,$Met(O_2)^{11}$] Substance P and $NK_2$ receptor selective agonist [${\beta}-Ala^8$]-neurokinin A (4-10) induced tonic contraction with superimposed phasic contractions of longitudinal oriented muscle strip and almost blocked by selective antagonist L-732,138 and GR 159897, respectively. But $NK_3$ receptor selective agonist senktide did not showed any effect. Nifedipine ($1{\mu}M$) abolished the contraction produced either by EFS or by the TK receptor agonists [$Sar^9$,$Met(O_2)^{11}$] Substance P or [${\beta}-Ala^8$]-neurokinin A (4-10). It is concluded that, in the longitudinal muscle of rat ileum, both $NK_1$ and $NK_2$ receptors modulated the responses to exogenous tachykinins, whereas $NK_1$ is mainly involved in NANC neuromuscular contraction.

Keywords

References

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