대한수의학회지 (Korean Journal of Veterinary Research)

  • 제43권3호
  • /
  • Pages.429-433
  • /
  • 2003
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

액체크로마토그래프/질량분석기를 이용한 소의 혈청 중 Ceftiofur의 분석

Determination of Ceftiofur in Bovine Serum by liquid Chromatography-Electrospray Mass Spectrometry

  • 임종환 (충남대학교 수의과대학) ;
  • 장범수 (한국화학연구원 생명의학연구부) ;
  • 박병권 (충남대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학)
  • Lim, Jong-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Jang, Beon-su (Medical Science Division, Korea Research Institute of Chemical Technology) ;
  • Park, Byung-kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University)
  • 심사 : 2003.07.02
  • 발행 : 2003.09.25
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초록

This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.

키워드

참고문헌

  1. Adams, H. R. Veterinary Pharmacology and Therapeutics, Iowa State University Press, Ames, 2001
  2. Brown, S. A., Chester, S. T., Speedy, A. K., Hubbard, V. L., Callahan, J. K., Hamlow, P. J., Hibbard, B. and Robb, E. J. Comparison of plasma pharmacokinetics and bioequivalence of ceftiofur sodium in cattle after a single intramuscular or subcutaneous injection. J. Vet. Pharmacol. Ther. 2000, 23(5), 273-280
  3. Beconi-Barker, M. G., Roof, R. D., Millerioux, L., Kausche, F. M., Vidmar, T. H., Smith, E. B., Callahan, J. K., Hubbard, V. L., Smith, G. A. and Gilbertson, T. J. Determination of ceftiofur and its desfuroylceftiofur-related metabolites in swine tissues by high-performance liquid chromatography. J. Chro- matogr. B Biomed Appl. 1995, 673(2), 231-244
  4. Fagerquist, C. K. and Lightfield, A. R. Confirmatory analysis of beta-lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2003, 17(7), 660-671
  5. Gaspar, A., Andrasi, M. and Kardos, S. Application of capillary zone electrophoresis to the analysis and to a stability study of cephalosporins. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2002, 775(2), 239-246
  6. Gilbertson, T. J., Mejeur, R. L., Yein, F. S. and Jaglan. P. S. Modified microbiological method for the screening of antibiotics in milk. J. Dairy Sci. 1995, 78(5), 1032-1038
  7. Holstege, D. M., Puschner, B., Whitehead, G. and Galey, F. D. Screening and mass spectral confirmation of beta-lactam antibiotic residues in milk using LC- MS/MS. J. Agric. Food Chem. 2002, 50(2), 406-411
  8. Hong, C. C., Lin, C. L., Tsai, C. E. and Kondo, F. Simultaneous identification and determination of residual penicillins by use of high-performance liquid chromato- graphy with spectrophotometric or fluorometric detectors. Am. J. Vet. Res. 1995, 56(3), 297-303
  9. Hornish, R .E. and Kotarski, S. F. Cephalosporins in veterinary medicine-ceftiofur use in food animals. Curr. Top. Med. Chem. 2002, 2(7), 717-731
  10. Keever. J, Voyksner, R. D. and Tyczkowska, K. L. Quantitative determination of ceftiofur in milk by liquid chromatography-electrospray mass spectrometry. J. Chromatogr. A. 1998, 794(1-2), 57-62
  11. Lin, C. E., Chen, H. W., Lin, E. C., Lin, K. S. and Huang H. C. Optimization of separation and migration behavior of cephalosporins in capillary zone electro phoresis. J. Chromatogr. A. 2000, 879(2), 197-210
  12. McNeilly, P. J., Reeves, V. B. and Deveau, E. J. Determination of ceftiofur in bovine milk by liquid chromatography. J. AOAC Int. 1996, 79(4), 844-847
  13. Mrestani, Y., Neubert, R., Schiewe, J. and Hartl, A. Application of capillary zone electrophoresis in cephalo- sporin analysis. J. Chromatogr. B. Biomed. Sci. Appl. 1997, 690(1-2), 321-326
  14. Navarre, C. B., Zhang, L., Sunkara, G., Duran, S. H. and Kompella. U. B. Ceftiofur distribution in plasma and joint fluid following regional limb injection in cattle. J, Vet. Pharmacol. Ther. 1999, 22(1), 13-19
  15. Okerman, L., De Wasch, K. and Van Hoof, J. Detection of antibiotics in muscle tissue with micro- biological inhibition tests: effects of the matrix. Analyst. 1998, 123(11), 2361-2365
  16. Salter, R. S., Legg, D., Ossanna, N., Boyer, C., Scheemaker, J., Markovsky, R. and Saul, S. J. Charm Safe-Level beta-Lactam Test for amoxicillin, ampicillin, ceftiofur, cephapirin, and penicillin G in raw commingled milk. J. AOAC Int. 2001, 84(1), 29-36
  17. Sorensen, L. K. and Snor, L. K. Determination of cephalosporins in raw bovine milk by high-performance liquid chromatography. J. Chromatogr. A. 2000, 882(1-2), 145-151
  18. Straub, R., Linder, M. and Voyksner, R. D. Determination of beta-lactam residues in milk using perfusive-particle liquid chromatography combined with ultrasonic nebulization electrospray mass spectrometry. Anal. Chem. 1994, 66(21), 3651-3658
  19. Sunkara, G., Navarre, C. B. and Kompella, U. B. Influence of pH and temperature on kinetics of ceftiofur degradation in aqueous solutions. J. Pharm. Pharmacol. 1999, 51(3), 249-255
  20. Zeng, S. S., Hart, S., Escobar, E. N. and Tesfai, K. Validation of antibiotic residue tests for dairy goats. J. Food Prot. 1998, 61(3), 344-349