Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Establishment of Neospora agglutination test for serologic diagnosis of neosporosis

Neospora응집반응을 이용한 네오스포라증의 혈청학적 진단

  • Kang, Min-Soo (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jae-Hoon (Department of Veterinary Medicine, Cheju National University) ;
  • Hwang, Woo-Suk (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Nam, Ho-Woo (Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University) ;
  • Youn, Hee-Jeong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Bae, Jong-Hee (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Dae-Yong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
  • Accepted : 2003.11.18
  • Published : 2003.12.25
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Abstract

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

Keywords

References

  1. 김대용, 황우석, 김재훈, 허권, 황의경, 이병천, 진영화,이재진, 최상호. Neospora에 의한 소 유산 발생 대한수의 학회지. 1997, 37, 607-612
  2. 김재훈, 황의경, 손현주, 진영화, 윤순식, 김대용. Neospora caninum에 의한 젖소의 반복유산. 대한수의학회지. 1998, 38, 853-858
  3. 안윤옥, 유근영, 박병주. 실용의학통계론. pp. 143-146, 서울대학교 출판부. 서울. 1996
  4. 이중근, 김재훈, 김진현, 이병천, 황우석, 윤희정, 남호우, 진영화, 김대용. 파라핀 블록 PCR을 이용한 소네오스포라 감염증의 진단법 확립. 대한수의학회지. 2001, 41, 381-385
  5. 허권, 김재훈, 황우석, 황의경, 진영화, 이병천, 배지선, 강영배, Yamane, I., 김대용. 간접형광항체법을 이용한 국내 젖소의 Neospora caninum에 대한 혈청학적 연구. 대한수의학회지. 1998, 38, 859-866
  6. Bae, J. S., Kim, D. Y., Hwang, W. S., Kim, J. H., Lee, N. S. and Nam, H. W. Detection of IgG antibody against Neospora caninum in cattle in Korea. Korean J. Parasitol. 2000, 38, 245-249
  7. Barr, B. C., Anderson, M. L., Woods, L. W., Dubey, J. P. and Conrad, P. A. Neospora-like protozoal infections associated with abortion in goats. J. Vet. Diagn. Invest. 1992, 4, 365-367
  8. Bjerkas, I., Mohn, S. F. and Presthus, J. Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Z. Parasitenkd. 1984, 70, 271-274
  9. Conrad, P. A., Sverlow, K. W., Anderson, M. L., Rowe, J. D., BonDurant, R., Tuter, G., Breitmeyer, R., Palmer, C., Thurmond, M., Ardans, A. A., Dubey, J. P., Duhamel, G. and Barr, B. Detection of serum antibody responses in cattle with natural or experimental Neospora infections. J. Vet. Diagn. Invest. 1993, 5, 572-578
  10. Desmonts, G. and Remington, J. S. Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity. J. Clin. Microbiol. 1980, 11, 562-568
  11. Dubey, J. P. Recent advances in Neospora and neosporosis. Vet. Parasitol. 1999, 84, 349-367
  12. Dubey, J. P., Hartley, W. J., Lindsay, D. S. and Topper, M. J. Fatal congenital Neospora caninum infection in a lamb. J. Parasitol. 1990, 76, 127-130
  13. Dubey, J. P., Lindsay, D. S., Adams, D. S., Gay, J. M., Baszler, T. V., Blagburn, B. L. and Thulliez, P. Serologic responses of cattle and other animals infected with Neospora caninum. Am. J. Vet. Res. 1996, 57, 329-336
  14. Dubey, J. P., Ricoulet, J., Lagourette, P., Georee, C., Longeart, L. and LeNet, J. L. Patal transplacental neosporosis in a deer (Cervus eldi siamensis). J. Parasitol. 1996, 82, 338-339
  15. Kim, J. H., Sohn, H. J., Hwang, W. S., Hwang, E. K., Jean, Y. H., Yamane, I. and Kim, D. Y. In vitro isolation and characterization of bovine Neospora caninum in Korea. Vet. Parasitol. 2000, 90, 147-154
  16. Marsh, A. E., Barr, B. C., Madigan, J., Lakritz, J., Nordhausen, R. and Conrad, P. A. Neosporosis as a cause of equine protozoal myeloencephalitis. J. Am. Vet. Med. Assoc. 1996, 209, 1907-1913
  17. McAllister, M. M., Dubey, J. R, Lindsay, D. S., Jolley, W. R., Wills, R. A. and McGuire, A. M. Dogs are definitive hosts of Neospora caninum. Int. J. Parasitol. 1998, 28, 1473-1478
  18. Packham, A. E., Sverlow, K. W., Conrad, P. A., Loomis, E. R, Rowe, J. D., Anderson, M. L., Marsh, A., Cray, C. and Barr, B. C. A modified agglutination test for Neospora caninum: development, optimization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 1998, 5, 467-473
  19. Pare, J., Hietala, S. K. and Thurmond, M. C. An enzyme-linked imniunosorbent assay (ELISA) for serological diagnosis of Neospora sp. infection in cattle. J. Vet. Diagn. Invest. 1995, 7, 352-359
  20. Romand. S., Thulliez, P. and Dubey, J. P. Direct agglutination test for serologic diagnosis of Neospora caninum infection. Parasitol. Res. 1998, 84, 50-53