Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Conjugated Linoleic Acid on Adipocyte Secreted Proteins in vitro

  • Ha, Jung-Heun (Department of Bioscience, School of Life and Food Sciences, Handong Global University) ;
  • Ahn, In-Sook (Department of Food Science and Nutrition, Pusan National University) ;
  • Byun, Jae-Min (Department of Bioscience, School of Life and Food Sciences, Handong Global University) ;
  • Do, Hyung-Ki (Department of Bioscience, School of Life and Food Sciences, Handong Global University) ;
  • Jung, Sun-Young (Department of Bioscience, School of Life and Food Sciences, Handong Global University) ;
  • Jeong, Jae-Hong (Department of Hotel Culinary Arts, Ansan Technical College) ;
  • Wahle, Klaus W.J. (Department of Human Nutrition and Health School of Life Sciences, The Robert Gorden University) ;
  • Park, Kun-Young (Department of Food Science and Nutrition, Pusan National University) ;
  • Do, Myoung-Sool (Department of Bioscience, School of Life and Food Sciences, Handong Global University)
  • Published : 2003.09.01
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Abstract

Conjugated linoleic acid (CLA) is a class of positional, geometric conjugated dienoic isomers of linoleic acid (LA). CLA activates the immune system, protects against tumorigenesis, and reduces the incidence of atherosclerosis. Trans-10, cis-12 CLA has specific effects on lipid metabolism, it has been shown to reduce body fat gain and regulates some adipocyte secreted proteins in vivo and in vitro. Here we report that a CLA mixture affects cytokine secretion from rat primary adipocytes. Rat primary adipocytes were treated with 1 mM, 100 $\mu$M, 1 $\mu$M or 100 nM CLA mixture doses; and leptin, tumor necrosis factor alpha (TNF a ), interleukin-6 (IL-6) and glycerol levels in the medium were measured. Leptin secretion was lower, TNF $\alpha$ secretion higher and IL-6 secretion did not change in response to the CLA mixture. Leptin and TNF $\alpha$ secretions did not change with CLA mixture treatment in a dose-dependent manner. In addition, the CLA mixture did not appear to enhance lipolysis in rat primary adipocytes. In conclusion, our study demonstrates that the decrease in leptin and increase in TNF $\alpha$ secretion in adipocytes treated with CLA mixture may be due to the apoptotic effect and to a reduction in peroxisome proliferator-activated receptor gamma (PPAR ${\gamma}$ ) ligands.

Keywords

References

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