Bulletin of the Korean Chemical Society

  • 제25권1호
  • /
  • Pages.109-114
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  • 2004
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Determination of Glimepiride in Human Plasma by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

  • Kim, Ho-Hyun (Department of Pharmacokinetics, Seoul Medical Science Institute, Seoul Clinical Laboratories (SCL), Department of Drug Development Service, BioCore Co., Ltd) ;
  • Chang, Kyu-Young (Department of Drug Development Service, BioCore Co., Ltd) ;
  • Lee, Hee-Joo (Department of Pharmacokinetics, Seoul Medical Science Institute, Seoul Clinical Laboratories (SCL), Department of Drug Development Service, BioCore Co., Ltd) ;
  • Han, Sang-Beom (College of Pharmacy, ChungAng University)
  • 발행 : 2004.01.20
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초록

A sensitive method for quantitation of glimepiride in human plasma has been established using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Glipizide was used as an internal standard. Glimepiride and internal standard in plasma sample was extracted using diethyl etherethyl acetate (1 : 1). A centrifuged upper layer was then evaporated and reconstituted with the mobile phase of acetonitrile-5 mM ammonium acetate (60:40, pH 3.0). The reconstituted samples were injected into a $C_{18}$ reversed-phase column. Using MS/MS in the multiple reaction monitoring (MRM) mode, glimepiride and glipizide were detected without severe interference from human plasma matrix. Glimepiride produced a protonated precursor ion ([M+H]$^+$) at m/z 491 and a corresponding product ion at m/z 352. And the internal standard produced a protonated precursor ion ([M+H]]$^+$) at m/z 446 and a corresponding product ion at m/z 321. Detection of glimepiride in human plasma by the LC-ESI/MS/MS method was accurate and precise with a quantitation limit of 0.1 ng/mL. The validation, reproducibility, stability, and recovery of the method were evaluated. The method has been successfully applied to pharmacokinetic studies of glimepiride in human plasma.

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피인용 문헌

  1. Determination of Glimepiride in Rat Serum by Rp-Hplc Method vol.02, pp.02, 2011, https://doi.org/10.4236/ajac.2011.22017
  2. Rapid and Specific Approach for Direct Measurement of Glimepiride in Human Plasma by LC-ESI-MS-MS Employing Automated 96 Well Format: Application to a Bioequivalence Study vol.50, pp.1, 2012, https://doi.org/10.1093/chromsci/bmr005
  3. Development and validation of RP-HPLC method for glimepiride and its application for a novel self-nanoemulsifying powder (SNEP) formulation analysis and dissolution study vol.5, pp.1, 2014, https://doi.org/10.1186/s40543-014-0027-0
  4. Development of Solid-Phase Extraction and HPLC Method for Simultaneous Estimation of Ilaprazole and Glimepiride in Rat Plasma: Application to Pharmacokinetic Studies pp.1945-239X, 2017, https://doi.org/10.1093/chromsci/bmw189
  5. Current literature in mass spectrometry vol.39, pp.10, 2004, https://doi.org/10.1002/jms.711
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  7. RP-LC Determination of the cis-Isomer of Glimepiride in a Bulk Drug Substance vol.66, pp.7-8, 2007, https://doi.org/10.1365/s10337-007-0356-x
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  9. Optimization of self-nanoemulsifying systems for the enhancement of in vivo hypoglycemic efficacy of glimepiride transdermal patches vol.11, pp.7, 2004, https://doi.org/10.1517/17425247.2014.906402
  10. Development and validation of the method for the detection of glimepiride via derivatization employing N-methyl-N-(trimethylsilyl) trifluoroacetamide using gas chromatography-mass spectrometry vol.11, pp.1, 2004, https://doi.org/10.1186/s41935-021-00217-7