Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effects of Chelidonii Herbal-acupuncture solution Anti-inflammatory in RAW 264.7 macrophages

백굴채약침액(白屈菜藥針液)이 LPS로 유도(誘導)된 RAW 264.7 대식세포(大食細胞)에서의 항염증효과(抗炎症效果)

  • Park, Dong-Cheon (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Park, Ji-Hyeon (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Lee, U-Kyung (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Leek, Jin-Kyu (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Seo, Il-Bok (Dept. of Anatomy, College of Oriental Medicine, Semyung University) ;
  • Kim, Ho-Hyun (Dept. of Physiology, College of Oriental Medicine, Semyung University) ;
  • Kim, Jeong-Seon (Dept. of Oriental Medical Food & Nutrition, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University)
  • 박동천 (세명대학교 한의과대학 경혈학교실) ;
  • 박지현 (세명대학교 한의과대학 경혈학교실) ;
  • 이우경 (세명대학교 한의과대학 경혈학교실) ;
  • 이진규 (세명대학교 한의과대학 경혈학교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실) ;
  • 김호현 (세명대학교 한의과대학 생리학교실) ;
  • 김정선 (세명대학교 한방식품영향학과) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실)
  • Published : 2004.06.27
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Abstract

Objectives : Recently, Herbal-acupuncture therapeutics has been used for the treatment of inflammatory diseases such as rheumatoid arthritis. Especially, we have been interested in chemical mediators concerned with inflammation such as prostaglandin, cytokine, nitrous oxide. The purpose of this study is investigated that the effect of Chelidonii Herbal-acupuncture solution in lipopolysaccharide-stimulated RAW 264.7 macrophages, performed several expeimental items : those are prostaglandin $E_2$, nitric oxide and cyclooxygenase-2. Methods : The cytotoxicity of Chelidonii Herbal-acupuncture solution in RAW 264.7 macrophages were measured by MTT-based cytotoxicity assay. In order to observe cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, RT-PCR was used. Prostaglandin $E_2$ formation and nitric oxide production was measured by competitive enzyme immunoassay and Griess assay. Results : 1.The MTT assay demonstrated that cytotoxic effect of Chelidonii Herbal-acupuncture solution in RAW 264.7 macrophages were not appeared before concentration of 1mg/ml. 2.Chelidonii Herbal-acupuncture solution inhibited cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages. 3. Chelidonii Herbal-acupuncture solution inhibited nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophages. 4. Chelidonii Herbal-acupuncture solution inhibited prostaglandin $E_2$ formation in lipopolysaccharide-stimulated RAW 264.7 macrophages. Conclusions : On the basis of these results, It was shown that Chelidonii Herbal-acupuncture solution is significantly able to inhibit the production of $PGE_2$ and NO, as well as COX-2 mRNA expression. Our results may provide new mechanism by which Chelidonii Herbal-acupuncture solution accounts for its beneficial effect on accelerating wound healing and anti-inflammation.

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