The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지

Upregulation of Heat Shock Proteins in the Kidney in Hypertension

  • Lee, Geon (Department of Physiology, Chonnam National University Medical School,Department of Pediatrics, Gwangju Christian Hospital) ;
  • Oh, Yoon-Wha (Department of Physiology, Chonnam National University Medical School,Department of Pediatrics, Gwangju Christian Hospital) ;
  • Lee, Jong-Un (Department of Physiology, Chonnam National University Medical School,Department of Pediatrics, Gwangju Christian Hospital)
  • Published : 2004.06.21
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was undertaken to determine the regulation of heat shock proteins (HSP) in the kidney in hypertension. Two-kidney, one clip (2K1C) or deoxycorticosterone acetate (DOCA)-salt hypertension was induced in male Sprague-Dawley rats. At weeks 1 and 4 after inducing the hypertension, the expression of HSP70, HSP32 and HSP25 was determined in the kidney by Western blot analysis. In 2K1C hypertension, the expression of HSP70, HSP32 and HSP25 was increased in the clipped kidney at both weeks 1 and 4. However, in the contralateral kidney, their expression was not significantly altered at week 1, but increased at week 4. In DOCA-salt hypertension, the expression of HSP remained unaltered in the remnant kidney at week 1, but significantly increased at week 4. These results indicate that HSP are differentially regulated in the kidney according to the duration and the model of hypertension.

Keywords

References

  1. Aizawa T, Ishizaka N, Taguchi J, Nagai R, Mori I, Tang SS, Ingelfinger JR, Ohno M. Heme oxygenase-1 is upregulated in the kidney of angiotensin II induced hypertensive rats: possible role in renoprotection. Hypertension 35: 800-806, 2000 https://doi.org/10.1161/01.HYP.35.3.800
  2. Chen HC, Guh JY, Tsai JH, Lai YH. Induction of heat shock protein 70 protects mesangial cells against oxidative injury. Kidney Int 56: 1270-1273, 1999 https://doi.org/10.1046/j.1523-1755.1999.00693.x
  3. Hamet P, Kaiser MA, Sun Y, Page V, Vincent M, Kren V, Pravenec M, Kunes J, Tremblay J, Samani NJ. HSP27 locus cosegregates with left ventricular mass independently of blood pressure. Hypertension 28: 1112-1117, 1996
  4. Hamet P, Kong D, Pravenec M, Kunes J, Kren V, Klir P, Sun YL, Tremblay J. Restriction fragment length polymorphism of hsp70 gene, localized in the RT1 complex, is associated with hy- pertension in spontaneously hypertensive rats. Hypertension 19: 611-614, 1992 https://doi.org/10.1161/01.HYP.19.6.611.a
  5. Hamet P, Malo D, Tremblay J. Increased transcription of a major stress gene in spontaneously hypertensive mice. Hypertension 15: 904-908, 1990 https://doi.org/10.1161/01.HYP.15.6.904
  6. Ishizaka N, Aizawa T, Ohno M, Usui SI, Mori I, Tang SS, Ingelfinger JR, Kimura S, Nagai R. Regulation and localization of HSP70 and HSP25 in the kidney of rats undergoing long-term administration of angiotensin II. Hypertension 39: 122-128, 2000 https://doi.org/10.1161/hy1201.096818
  7. Ishizaka N, de Leon H, Laursen JB, Fukui T, Wilcox JN, De Keulenaer G, Griendling KK, Alexander RW. Angiotensin II-induced hypertension increases heme oxygenase-1 expression in rat aorta. Circulation 96: 1923-1929, 1997 https://doi.org/10.1161/01.CIR.96.6.1923
  8. Johnson RA, Teran FJ, Durante W, Peyton KJ, Johnson FK. Enhanced heme oxygenase-mediated coronary vasodilation in Dahl salt-sensitive hypertension. Am J Hypertens 17: 25-30, 2004 https://doi.org/10.1016/j.amjhyper.2003.08.009
  9. Kohane DS, Sarzani R, Schwartz JH, Chobanian AV, Brecher P. Stress-induced proteins in aortic smooth muscle cells and aorta of hypertensive rats. Am J Physiol 258: H1699-705, 1990
  10. Komatsuda A, Wakui H, Oyama Y, Imai H, Miura AB, Itoh H, Tashima Y. Overexpression of the human 72 kDa heat shock protein in renal tubular cells confers resistance against oxidative injury and cisplatin toxicity. Nephrol Dial Transplant 14: 1385- 1390, 1999 https://doi.org/10.1093/ndt/14.6.1385
  11. Lu D, Maulik N, Moraru II, Kreutzer DL, Das DK. Molecular adaptation of vascular endothelial cells to oxidative stress. Am J Physiol 264: C715-722, 1993 https://doi.org/10.1152/ajpcell.1993.264.3.C715
  12. Nath KA, Balla G, Vercellotti GM, Balla J, Jacob HS, Levitt MD, Rosenberg ME. Induction of heme oxygenase is a rapid, protective response in rhabdomyolysis in the rat. J Clin Invest 90: 267-270, 1992 https://doi.org/10.1172/JCI115847
  13. Rogalla T, Ehrnsperger M, Preville X, Kotlyarov A, Lutsch G, Ducasse C, Paul C, Wieske M, Arrigo AP, Buchner J, Gaestel M. Regulation of Hsp27 oligomerization, chaperone function, and protective activity against oxidative stress/tumor necrosis factor alpha by phosphorylation. J Biol Chem 274: 18947-18956, 1999 https://doi.org/10.1074/jbc.274.27.18947
  14. Seki T, Naruse M, Naruse K, Yoshimoto T, Tanabe A, Seki M, Tago K, Imaki T, Demura R, Demura H. Induction of heme oxygenase produces load-independent cardioprotective effects in hypertensive rats. Life Sci 65: 1077-1086, 1999 https://doi.org/10.1016/S0024-3205(99)00338-0
  15. Shiraishi F, Curtis LM, Truong L, Poss K, Visner GA, Madsen K, Nick HS, Agarwal A. Heme oxygenase-1 gene ablation or expression modulates cisplatin-induced renal tubular apoptosis. Am J Physiol 278: F726-F736, 2000
  16. Slepian MJ, Massia SP, Whitesell L. Pre-conditioning of smooth muscle cells via induction of the heat shock response limits proliferation following mechanical injury. Biochem Biophys Res Commun 225: 600-607, 1996 https://doi.org/10.1006/bbrc.1996.1217
  17. Smoyer WE, Ransom R, Harris RC, Welsh MJ, Lutsch G, Benndorf R. Ischemic acute renal failure induces differential expression of small heat shock proteins. J Am Soc Nephrol 11: 211-221, 2000
  18. Turman MA, Rosenfeld SL. Heat shock protein 70 overexpression protects LLC-PK1 tubular cells from heat shock but not hypoxia. Kidney Int 55: 189-197, 1999 https://doi.org/10.1046/j.1523-1755.1999.00251.x
  19. Xu Q, Ganju L, Fawcett TW, Holbrook NJ. Vasopressin-induced heat shock protein expression in renal tubular cells. Lab Invest 74: 178-187, 199
  20. Xu Q, Li DG, Holbrook NJ, Udelsman R. Acute hypertension induces heat-shock protein 70 gene expression in rat aorta. Circulation 92: 1223-1229, 1995 https://doi.org/10.1161/01.CIR.92.5.1223