Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Photoprotective Effect and Antioxidative Activity from Different Organs of Morus Bombycis Koidzumi

부위별 산뽕나무의 광보호효과 및 항산화 활성

  • 사재훈 (강원도 보건환경연구원 식의약품분석과) ;
  • 김영선 (강원대학교 생명공학부) ;
  • 신인철 (강원도 보건환경연구원 식의약품분석과) ;
  • 심태흠 (강원도 보건환경연구원 식의약품분석과) ;
  • 왕명현 (강원대학교 생명공학부)
  • Published : 2004.09.30
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Abstract

This study was investigated antioxidatve activity for the purpose of developing antioxidant from Morus bombycis Koidzumi. Antioxidant activities of four different organs of Morus bombycis Koidzumi such as fruit, leaf, stem, and root were examined by radical scavenging effect with 1,1-diphenyl-2-picrylhydrazyl (DPPH). 80% methanol extract from the stem showed strongly antioxidative activity and 80% Ethanol extracts from the root, stem, and fruit had high antioxidative activity among 24 samples tested. The 80% ethanol extract has strong absorbency at UVA region (350 nm). The ethyl acetate (EtOAc) fraction exhibited antioxidative activity with $IC_{50}$ of $15.0\;{\mu}g/ml$ similar to those of synthetic antioxidant, BHT The EtOAc fraction has a good absorbency property as synthetic filter. In the absorbance of various extracts, the 80% ethanol and ethyl acetate extracts from the root of Morus bombycis Koidzumi showed higher absorbancy at 285 nm. The ethyl acetate fraction from the root of Morus bombycis Koidzumi contained total phenolic compounds of 654.8 mg/100 g. These results indicate that phenolic compounds are the major was biological components in the root of morus bombycis Koidzumi extracts. Considering these biological activities, the extracts of Morus bombycis Koidzumi showed a possibility to be used as a new material for natural anti-oxidants and substitutes for synthetic UV sunscreen agents.

산뽕나무의 생리활성 기능을 탐색하기 위하여 메탄올, 에탄올, 클로로포름, 80% 메탄올, 80% 에탄올 및 추출물을 조제하였고, 산뽕나무 뿌리의 80% 에탄올 추출물로부터 n-Hexane, $CHCl_3$, EtOAc. n-BuOH 및 water 분획물을 제조하였다. 얻어진 추출물과 분획물에 대하여 DPPH 자유라디칼 소거법에 의한 항산화성 효과와 UVB 및 UVA 영역에서의 자외선 흡수 효과를 검토하였다. 산뽕나무 추출물의 수소전자공여능$(IC_{50})$을 측정한 결과 추출물에서는 80% 메탄올 줄기 추출물, 80% 열매 추출물, 80% 뿌리 추출물, 80% 메탄올 및 추출물에서 높은 항산화 활성을 나타내었다. 자외선 차단효과에서는 80% 에탄올 뿌리 추출물 및 80% 메탄올 추출물에서 매우 높은 자외선 흡수력이 높게 측정되었다. 항산화 효과 및 자외선 차단효능이 탁월한 산뽕나무 뿌리의 80% 에탄올 추출물을 대상으로 용매 분획물을 제조하였다. 에틸아세테이트 분획물$(IC_{50}\;:\;15.0\;{\mu}g/ml)$이 가장 우수한 항산화 효과를 나타내었으며, 자외선 차단효과에서도 에틸아세테이트 분획물(E%cm: 223.4)이 자외선 B영역에서 매우 높은 흡수력을 나타내었다. 현재 산뽕나무 부리의 에틸아세테이트 분획물을 대상으로 활성물질의 분리 및 구조규명에 관한 연구를 진행중에 있다.

Keywords

References

  1. Harman, D. (1956) Aging: a theory based on free radical and radiation chemistry. J. Gerontol' 11: 298-300 https://doi.org/10.1093/geronj/11.3.298
  2. Richter, C., Park, J. W., and Ames, B. N. (1988) Normal oxidative damage to mitochondrial and nuclear DNA is extensive. Proc. Natl. Acad. Sci. USA, 85: 6465-6467 https://doi.org/10.1073/pnas.85.17.6465
  3. Oliver, C. N., Ahn, B. W., Moerman, E. J., Golgstein, S., and Stdtman, E. R. (1987) Age realated changes in oxidized proteins. J. Biol. Chem. 262: 5488-5491
  4. Brunk, U T., Jones, C. B., and Sohal, R. S. (1992) A novel hypothesis of lipofuschinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. Mutat. Res. 275: 395-403 https://doi.org/10.1016/0921-8734(92)90042-N
  5. Bryce, G. F. (1993) The effects of UV radiation on skin connective tissue, In oxidative stress in Dermatology, (J. Fuchs and L. Packer, eds), 105-125. Marcel Dekker, Inc., N.Y.
  6. Black, H. (1987) Potential involvement of free radical reactions in ultraviolet light-mediated cutaneous damage. Photochern. Photobiol. 46: 213-221 https://doi.org/10.1111/j.1751-1097.1987.tb04759.x
  7. Taira, H., Mimura, K, Yoneya, T., Hagi, A., Murakami, A., and Makino, K (1992) Hydroxyl radical formation by UVirradiated epidermal cells. J. Biochem. 111: 693-695 https://doi.org/10.1093/oxfordjournals.jbchem.a123820
  8. Dalle-Carbonare, M. and Pathak, M. A. (1992) Skin photosensitizing agents and the role of oxygen species in photoaging. Photochem. Photobiol' 14: 105-124 https://doi.org/10.1016/1011-1344(92)85086-A
  9. Jurkiewicz, B. A., Bissett, D. L., and Bootner, G. R. (1995) Effect of topically applied tocopherol on ultraviolet radiationmediated free radical damage in skin. J. Invest. Derrnatol' 104: 484-488 https://doi.org/10.1111/1523-1747.ep12605921
  10. Witt, E. H., Motchnik, P, and Packer, L. (1993) Evidence for UV light as an oxidative stress in dermatology, (1. Fuchs and L. Packer, eds), 29-47. Marcel Dekker, Inc., N.Y.
  11. Branen, A. L. (1975) Toxicological and biochemistry of butylated hydroxytoluene, butylated hydroxyanisole. J. Arn. Oil Chem. Soc. 52: 59-63 https://doi.org/10.1007/BF02901825
  12. Fukuda, Y. and Nagata, M. (1986) Chemical aspects of the antioxidative activity of roasted sesame seed oil and the effect of using the oil for frying. Agric. Biol. Chem. 50: 857-861 https://doi.org/10.1271/bbb1961.50.857
  13. Hudson, B. and Lewis, J. (1987) Polyhydroxyl flavonoid antioxidants for edible oil phospholipid as synergist. Food Chem. 19: 537-541
  14. Omaye, S. T., Reddy, K. A., and Cross, C. E. (1977) Effect of bytylated hydroxytoluene and other antioxidants on mouse lung metabolism. J. Toxicol. Environ. Health 3: 829-836 https://doi.org/10.1080/15287397709529617
  15. Lim, D. K, Choi, U, and Shim, D. H. (1996) Antioxidative activity of ethanol extract from Korean medicinal plants. Kor, J. Food Sci. Technol. 28: 83-89
  16. Kim, H. K., Kim, Y. E., Do, J. R., Lee, Y. C., and Lee, B. Y. (1995) Antioxidative activity and physiological activity of some Korean medicinal plants. Kor. J. Food Sci. Technol. 27: 80-85
  17. Cha, J. Y. and Cho, Y. S. (1999) Effect of potato polyphenolics on lipid peroxidation in rats. J. Kor. Soc. Food Nutr. 28: 1131-1136
  18. Kim, M. H., Kim, M. C., Park, J. S., Park, E. J., and Lee, J. O. (1999) Determination of antioxidants contents in various plants used as tea materials. Kor. J. Food Sci. Technol. 31: 273-279
  19. 김지영, 맹영선, 이기영(1995) 다양한 용매를 이용한 대두 추출물의 항산화 효과. Korean J. Food Sci. Technol. 27: 635-639
  20. 서영호, 김인종, 이안수, 민황기(1999) 찰옥수수의 전자공여작용과 페놀성화 Tocophrol 및 Carotenoids의 함량. Korean J. Food Sci. Technol. 31: 591-585
  21. Bobin, M. F., Raymond, M., and Martini, M. C. (1994) UVA and UVB absorption properties of natural products. Cosmetics & Toiletries 109: 63-70
  22. 이기동, 김정숙, 배재오, 윤형식(1992) 쑥의 물추출물과 에테르추출물의 항산화 효과. 한국영양식량학회지 21: 17-22
  23. 이미현, 정재홍, 오만진(1992) 도토리 gallic acid의 항산화성. 한국영양식량학회지 21: 693-700
  24. 최홍식, 이정수, 문갑순, 박건영(1993) 양조간장에서 리한갈색물질의 항산화성. 한국영양식량학회지 22: 565-569
  25. 지청일, 변한석, 강진훈, 이태기, 김선봉, 박영호(1992) 식물대두유에 대한 향신료 추출물의 항산화작용. 한국영양식량학회지 21: 551-556
  26. 여생규, 안철우, 이용우, 이태기, 박영호, 김선봉(1995) 녹차, 오룡차 및 홍차 추출물의 항산화 효과. 한국영양식량학회지 24: 299-304