Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Optimal Culture Conditions for in vitro Propagation of Orostachys japonicus and Enhancement of Polysaccharide Production

  • Kim, Won-Jung (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Division of Forest Science, Gyeongsang National University) ;
  • Park, Dong-Jin (Division of Forest Science, Gyeongsang National University) ;
  • Huh, Gyung-Hye (Genome Research Center, Inje University) ;
  • Lee, Byung-Hyun (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, Myung-Suk (Institute of Agriculture & Life Science, Gyeongsang National University)
  • Published : 2004.05.30
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Abstract

Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. The highest growth yield was achieved in 1/2 MS medium, while the lowest growth yield was obtained in 4 MS medium. The patterns of polysaccharide formation were a little similar in all cases, but on MB5 medium, the po]ysaccharide contents of plant were higher than others. Among the modified nitrate levels, effective growth level were obtained in 1/4 N and 1/2 N. High contents of polysaccharide were obtained in 4 N. Different concentration of potassium and calcium did not improve the growth and polysaccharide production. The micropropagated shoots were successfully acclimatized artificial soils.

Keywords

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