Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Physical Mapping of rDNAs Using McFISH in Anemarrhena asphodeloides Bunge

지모에서 McFISH를 이용한 rDNAs의 물리지도 작성

  • Kim, Soo-Young (School of Biosciences & Biotechnology, Chungnam Natl. Univ.) ;
  • Choi, Hae-Woon (School of Biosciences & Biotechnology, Chungnam Natl. Univ.) ;
  • Bang, Jae-Wook (School of Biosciences & Biotechnology, Chungnam Natl. Univ.)
  • Published : 2004.12.31
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Abstract

Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.

약용식물로 재배되고 있는 지모를 대상으로 McFISH 기법을 이용하여 45S와 5S rDNA 유전자의 염색체상의 위치를 확인하여 물리지도를 확립하였다. 2쌍의 45S rDNA는 1번 염색체의 단완 말단과 3번 염색체의 동원체 부위에서 관찰되었고, 1번 염색체의 signal이 3번 염색체에서의 signal보다 더 강하게 나타났다. 한 쌍의 5S rDNA signal은 45S rDNA signal과 함께 3번 염색체의 동원체 부위에서 관찰되었다.

Keywords

References

  1. Bae KH (2000) The Medicinal Plants of Korea. Kyohaksa, Seoul, Korea. p. 546
  2. Bae KH (2003) Illustrated Book of Health for Longevity. Kyohaksa Seoul, Korea. p. 363
  3. Darlington CD, Wylie AP (1955) Chromosome Atlas of Flowering Plants. 2nd Ed. George Allen & Unwin Ltd, London. p. 365
  4. Fransz PF, Amstrong S, De Jong JH, Parnell LD (2000) Integrated cytogenetic map of chromosome arm 45S of Arabidopsis thaliana: Structural organization of heterochromatic knob and centromere region. Cell 100:367-376 https://doi.org/10.1016/S0092-8674(00)80672-8
  5. lchiki H, Miura T, Kubo M, Ishihara E, Komatsu Y, Tanigawa K, Okada M (1998) New antidiabetic compounds, mangiferin and its glucoide. Biol. Pharm. Bull. 21:1389-1390 https://doi.org/10.1248/bpb.21.1389
  6. Jiang J, Gill BS (1994) Nonisotopic in situ hybridization and plant genome mapping: The first ten years. Genome 37:717-725 https://doi.org/10.1139/g94-102
  7. Juliano O,Jonathan C, Gill H, Heslop-Harrison JS (1998) Molecular cytogenetics of Musa species, cultivars and hybrids: Location of 18S-5.8S-25S and 5S rDNA and telomere-like sequences. Ann. Bot. 82:243-248 https://doi.org/10.1006/anbo.1998.0674
  8. Kim SM, Park CK, Seong JD, Kim HT, Han SI, Kwack YH (1999) Isolation and HPLC analysis of timosaponin A III from rhizomes of Anemarrhrna asphodeJoides BUNGE. Korean J. Medicinal Crop Sci. 7:45-50
  9. Kim SY, Lim YP, Bang JW (1998) Cytogenetic analysis of Brassica campestris var. pekinensis using C-banding and FISH. Korean J. Genetics 20:285-294
  10. Kim SY, Koo DH, Bang JW (2002) Karyotype analysis of Anemarrhena asphodeJoides Bunge. Korean J. Medicinal Crop Sci. 10:144-146
  11. Koo DH, Hur YK, Bang JW (2003) FISH mapping of rDNAs and repeated DNA sequences on pachytene chromosomes of cucumber (Cucumis sativus L.). Plant & Animal genome XI; p.145
  12. Koo DH, Kim SY, Bang KH, Seong NS, Bang JW (2003) Cytogenetic analyses of Angelica plants using Feulgen staining and multicolor fluorescence in situ hybridization. Korean J. Plant Biotech. 30:123-127 https://doi.org/10.5010/JPB.2003.30.2.123
  13. Laptan NLV, Ganal MW, Tanksley SD (1989) Somatic chromosome karyotype of tomato based on in situ hybridization of the TAGI satellite repeat. Genome 32:992-998 https://doi.org/10.1139/g89-543
  14. Lee SH, Ryu SY, Choi SU, No ZS, Kim SK, Lee CO, Ahn lW (1995) Antitumor agent from the rhizome of Anemarrhena asphodeloides. Korean J. Pharmacogn. 26:47-50
  15. Leitch IJ, Heslop-HarrisonJS (1993) Physical mapping of four sites of 5S ribosomal DNA sequences and one site of the Xamylase 2 gene in barley (Hordeum vulgare). Genome 36:517-523 https://doi.org/10.1139/g93-071
  16. Ma B, Wang B, Dong], Yan X, Zhang H, Tu A (1997) New spirostanol glycosides rom Anemarrhena asphodeloides. Planta Med. 63:376-379 https://doi.org/10.1055/s-2006-957710
  17. Maluszynska J, Heslop-Hanison JS (1991) Localization of tandernly repeated DNA sequences in Arabidopsis thaliana. Plant J .1:159-166 https://doi.org/10.1111/j.1365-313X.1991.00159.x
  18. Mukai Y, Endo TR, Gill BS (1991) Physical mapping of the 18S26S rRNA multigene family in common wheat: identification of a new locus. Chromosoma 100:71-78 https://doi.org/10.1007/BF00418239
  19. Murata M, Heslop-Harrison JS, Motoyoshi F (1997) Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones. Plant J. 12:31-37 https://doi.org/10.1046/j.1365-313X.1997.12010031.x
  20. Sato D (1942) Karyotype alteration and phylogeny in Liliaceae and allied families. Jap. J. Bot. 12:57-161
  21. Schwarzacher T, Leitch AR, Heslop-Harrison]S (1989) In situ hybridization and the architecture of the nucleus. Trans. R. Microscop Soc. 1:669-674
  22. Yamamoto M, Mukai Y (1989) Application of fluorescence in situ hybridization to molecular cytogenetics of wheat. Wheat Inf. Serv. 69:30-33