시판 양념갈비에 오염된 병원성 미생물의 감마선 감수성 및 감마선 조사된 갈비의 관능적 품질특성

Radio-sensitivity of Contaminated Pathogens in Marinated Beef Rib (Galbi) Treated with ${\gamma}-Irradiation$ and Its Sensory Properties

  • 이나영 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 조철훈 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 강호진 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 김동수 (한국원자력연구소 방사선식품.생명공학연구팀) ;
  • 변명우 (한국원자력연구소 방사선식품.생명공학연구팀)
  • Lee, Na-Young (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Cho, Cheo-Hoon (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Kang, Ho-Jin (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Kim, Dong-Soo (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Byun, Myung-Woo (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute)
  • 발행 : 2004.02.28

초록

시중에서 판매되는 양념갈비의 식품매개 병원성 미생물의 오염정도를 확인하였으며, 포장방법을 달리하여 포장하여 감마선 조사한 후 저장기간에 따른 갈비의 미생물 안전성 및 조사선량에 따른 소비자 기호도를 조사하였다. 식품매개 병원성 미생물의 오염은 B. cereus, S. typhimurium, E. coli 및 S. aureus는 모두 3-4 log cycle정도로 검출되어 시중에서 판매되는 갈비의 식품매개 병원성 미생물의 오염정도가 심각한 것으로 나타났다. 갈비의 조사선량에 따른 변화는 7.5 kGy로 감마선 조사할 경우 B. cereus를 제외하고 S. typhimurium 및 S. aureus는 검출되지 않았으며, E. coli는 0일째에 1.47 log cycle정도 검출되었으나 $4^{\circ}C$ 저장 중에는 검출되지 않았다. 멸균된 갈비와 양념갈비의 방사선 감수성은 B. cereus가 방사선 조사에 대한 내성이 가장 강한 균주로 나타났다. 감마선 조사에 따른 갈비의 관능 특성은 조리되지 않은 갈비의 경우 항은 비조사구가 높은 선호도를 보였으며 색은 조사구가 높은 평가를 받았다. 조리된 갈비의 경우 함기포장한 갈비는 조직감, 맛 및 전체적인 기호도에서 유의차를 나타내지 않았고, 진공포장한 시료의 경우 맛은 7.5kGy로 조사한 갈비를 제외한 0. 2.5 및 5.0kGy로 조사된 갈비는 높은 평가를 받았으며 전체적인 기호도는 0 및 2.5kGy로 조사된 갈비가 가장 높은 선호도를 보였다.

Radio-sensitivity of pathogens in commercial marinated beef ribs, Galbi, and effect of irradiation on microbiological safety and sensory properties of marinated beef rib were investigated. Commercial raw marinated beef ribs were highly contaminated by pathogenic microorganisms including Bacillus cereus, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus, ranged from $10^{3}\;to\;10^{4}\;CFU/g$. Gamma irradiation reduced pathogen count during storage. No viable cells of pathogens were observed in sample irradiated at 7.5 kGy except for Bacillus cereus. Radio-sensitivity of inoculated pathogens in marinated beef ribs and commercial one showed $D_{10}$ value of 0.54-0.60 and 2.15-2.98, respectively, In sensory evaluation irradiated sample showed highest color and overall acceptance among treatments (p<0.05). Results indicate low dose (2.5-5.0 kGy) irradiation is effective for ensuring safety of marinated beef ribs without decreasing sensory quality.

키워드

참고문헌

  1. Yook HS, Lee JW, Lee HJ, Kim JG, Kim KP, Byun MW. Effect of gamma irradiation on the protein solubility, pruge loss and shear force of beef. Korean J. Food Sci. Technol. 31: 665-671 (1999)
  2. Jo C, Kim DH, Shin MG, Kang IJ, Byun MW. Irradiation effect on Bulgogi sauce for making commercial Korean traditional meat product, Bulgogi. Radiat. Phys. Chem. 68: 851-856 (2003) https://doi.org/10.1016/S0969-806X(03)00328-1
  3. Chung MS, Kang HD, Um BY, Kim YJ, Lee M. Effects of low dose electron-beam on the beef aging. Korean J. Anim. Sci. 40: 193-202 (1998)
  4. Buzby JC, Roberts T. ERS estimates U.S. food-borne disease costs. Food Rev. 24: 37-42 (2001)
  5. Byun MW, Lee JW. Application of irradiation technology for food safety and security. Food Sci. Ind. 36: 25-41 (2003)
  6. Aziz NH, Mahrous SR, Youssef BM. Effect of gamma-ray and microwave threatment on the shelf-life of beef products stored at 5$\circ$C. Food Control 13: 437-444 (2002) https://doi.org/10.1016/S0956-7135(01)00049-4
  7. Kim KJ, Min JS, Lee SO, Jang A, Jang SH, Cheon YH, Lee M. Effect of natrual or phosphates on quality improvement of the low-grade seasoned hanwoo ribs. J. Anim. Sci. Technol. 45: 309-318 (2003) https://doi.org/10.5187/JAST.2003.45.2.309
  8. Lee JW, Yook HS, Kim JH, Kim KP, Lee HJ, Byun MW. Use of gamma irradiation for improving quality and assuring safety of meat products. J. Korean Soc. Food Sci. Nutr. 28: 858-864 (1999)
  9. Montgomery JL, Parrish FC Jr, Olson DG, Dickson JS, Niebuhr S. Storage and packaging effects on sensory and color characteristics of ground beef. Meat Sci. 64: 357-363 (2003) https://doi.org/10.1016/S0309-1740(02)00171-7
  10. Yook HS, Lee KH, Lee JW, Kang KO, Byun MW. Effect of gamma irradiation on lipid oxidation of Korean beef. Korean J. Food Sci. Technol. 30: 1179-1183 (1998)
  11. Anomymous. Irradiation enhances food safety and quality. Food Prot. Trends 23: 593-574 (2003)
  12. Byun MW. Application and aspect of irradiation technology in food industry. Food Sci. Ind. 30: 89-100 (1997)
  13. SAS Institute, Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1990)
  14. Gu DW, Chung CI, Jeong DK, Nam ES. Contamination of Listeria spp. in market beef. J. Fd. Hyg. Safety 10: 89-95 (1995)
  15. Thayer DW, Boyd G, Fox JB, Lakritz L. Effects of NaCl, sucrose, and water content on survival of Salmonella typhimurium on irradiated pork and chicken. J. Food Prot. 58: 490-496 (1995) https://doi.org/10.4315/0362-028X-58.5.490
  16. Yook HS, Kim S, Lee KH, Kim YJ, Kim KP, Byun MW. Gamma-radiation sensitivity of pathogenic bacteria in beef. Korean J. Food Sci. Technol. 30: 1432-1438 (1998)
  17. Kim S, Yook HS, Lee JW, Choi C, Byun MW. Sterilization of Escherichia coli O157:H7 contaminated beef by gamma irradiation. Korean J. Food Sci. Technol. 30: 1209-1213 (1998)
  18. Thayer DW, Boyd G. Control of enterotoxic Bacillus cereus on poultry or red meats and in beef gravy by gamma irradiation. J. Food Prot. 57: 758-764 (1994) https://doi.org/10.4315/0362-028X-57.9.758
  19. Byun MW, Lee JW, Jo C, Yook HS. Quality properties of sausage made with gamma-irradiated natural pork and lamb casing. Meat Sci. 59: 223-228 (2001) https://doi.org/10.1016/S0309-1740(01)00062-6
  20. Kim CJ, Jeong JY, Lee ES, Song HH. Studies on the improvement of quality and shelf-life of traditional marinated beef (Galbi) as affected by packaging method during storage at -1$\circ$C. Korean J. Food Sci. Technol. 34: 792-798 (2002)