Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Simultaneous Determination of Vitamin A and E in Infant Formula by Rapid Extraction and HPLC with Photodiode Array Detection

신속추출법 및 PDA-HPLC에 의한 조제분유 중 비타민 A, E의 동시분석

  • Kwak, Byung-Man (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Lee, Ki-Woong (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Ahn, Jang-Hyuk (Research and Development Center, Namyang Dairy Products Corporation) ;
  • Kong, Un-Young (Research and Development Center, Namyang Dairy Products Corporation)
  • 곽병만 (남양유업(주) 중앙연구소) ;
  • 이기웅 (남양유업(주) 중앙연구소) ;
  • 안장혁 (남양유업(주) 중앙연구소) ;
  • 공운영 (남양유업(주) 중앙연구소)
  • Published : 2004.04.30
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Abstract

Rapid and simple method was developed for simultaneous determination of vitamins A and E contents in infant formula. Vitamins A and E were extracted by PDA-HPLC with reversed phase column using organic solvent, and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with results of Food Standards Codex and AOAC method for evaluation of developed method, Vitamins A and E contents in CRM determined by developed method were within certified range of standard values. Developed method has great advantages of simple and rapid sample preparation and simultaneous determination of vitamins A and E by PDA-HPLC using reversed phase column.

본 연구에서는 조제분유 중에 영양강화를 위해 첨가하는 비타민 A와 E의 함량측정을 위해 각기 다른 실험방법을 사용하지 않고 2가지 비타민을 동시에 분석하는 신속분석법을 위와 같이 수행하였다. 비타민 A와 E를 유기용매로 동시에 신속하게 추출하고 역상컬럼과 PDA-HPLC를 이용하여 각각의 성분으로 모두 분리한 후 동시에 검출하는 방법을 사용하였다. 국제표준인증물질 및 조제분유를 시료로 사용하여 본 연구의 실험방법에 의해 측정된 값을 식품공전방법 및 AOAC방법에 의한 측정값과 비교한 결과 유사한 측정결과를 얻을 수 있었다. 또한 본 연구의 실험방법에 의하여 수행한 국제인증표준물질중의 비타민 A와 E의 함량측정 결과는 인증된 표준값내의 결과를 보여주었다. 따라서, 비타민 A또는 E를 강화한 분유, 이유식 등의 분말 유제품 중에서 비타민 A와 E의 함량을 측정 하고자 할 때 한정된 장비와 인력으로 각각의 2가지 실험방법을 수행하기가 어렵거나 시간단축이 필요한 경우, 본 연구에서 수행한 실험방법과 같이 시료 전처리를 간단하고 신속하게 수행할 뿐만 아니라 역상컬럼과 PDA-HPLC에 의해 비타민 A와 E를 동시에 분석함으로써 보다 효율적인 분석을 진행 할 수 있을 것으로 사료된다.

Keywords

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