Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Antioxidant Effect of Rosa davurica Pall Extract on Oxidation of Human Low Density Lipoprotein (LDL)

사람의 저밀도 지방단백질의 산화에 대한 생열귀나무 추출물의 항산화 효과

  • Sa, Jae-Hoon (Gangwon Research Institute of Health and Environment) ;
  • Lee, Woan (Gangwon Research Institute of Health and Environment) ;
  • Shin, In-Cheol (Gangwon Research Institute of Health and Environment) ;
  • Jeong, Kyung-Jin (Gangwon Research Institute of Health and Environment) ;
  • Shim, Tae-Heum (Gangwon Research Institute of Health and Environment) ;
  • Oh, Heung-Seok (Gangwon Research Institute of Health and Environment) ;
  • Kim, Yong-Jin (Gangwon Research Institute of Health and Environment) ;
  • Cheung, Eui-Ho (Gangwon Research Institute of Health and Environment) ;
  • Kim, Gwang-Gee (Jungsun Agriculture Technology Center) ;
  • Choi, Dae-Sung (Jungsun Agriculture Technology Center)
  • 사재훈 (강원도보건환경연구원) ;
  • 이완 (강원도보건환경연구원) ;
  • 신인철 (강원도보건환경연구원) ;
  • 정경진 (강원도보건환경연구원) ;
  • 심태흠 (강원도보건환경연구원) ;
  • 오흥석 (강원도보건환경연구원) ;
  • 김영진 (강원도보건환경연구원) ;
  • 정의호 (강원도보건환경연구원) ;
  • 김광기 (정선군농업기술센터) ;
  • 최대성 (정선군농업기술센터)
  • Published : 2004.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Antioxidant effects of Rosa davurica Pall extract on copper-mediated LDL oxidative modification were investigated. Oxidation products of LDL were determined based on TBA value, formation of conjugate diene, and apolipoprotein carbonyl value. As revealed through TBA values, ethyl acetate and butanol fractions of R. davurica Pall root showed strong antioxidant effect, with 85.3 and 93.2% inhibitions at $30\;{\mu}g/mL$ each, respectively. Ethyl acetate and butanol fractions at $30\;{\mu}g/mL$ inhibited LDL oxidation up to 8 hr. Conjugate diene formation by lipid oxidation with $Cu^{2+}$ addition in ethyl acetate and butanol fractions decreased 2.2-and 5.6-fold, respectively, compared to control. Carbonyl value decreased in the presence of butanol and ethyl acetate fractions. Methanol and ethyl acetate extracts of R. davurica Pall root showed higher absorbancy at 285 nm. Ethanol extract of R. davurica Pall root and stem contained 10.6 g/100 g total phenolic compounds. Results reveal phenolic compound as major biological component in R. davurica Pall extracts. Ethyl acetate and butanol fraction showed strongest antioxidant effect on LDL oxidation.

비타민-C를 다량 함유하고 있는 생열귀나무의 자원화를 위한 연구로서 페놀성 화합물의 함량 및 사람의 저밀도지방단백질의 산화에 대한 항산화 활성을 측정하였다. 생열귀나무 추출물의 페놀성 화합물 함량을 측정한 결과, 모든 부위에서 100g당 8.3-10.6g으로 매우 높은 양을 함유하고 있었다. 또한, 생열귀나무 추출물 및 분획물에 대한 항산화 물질의 함량을 측정 한 결과, 생열귀나무 뿌리 메탄올 추출물과 뿌리의 에틸아세테이트 및 부탄을 분획물이 285nm에서 흡수를 나타내는 페놀성 화합물을 다량 함유하고 있음을 확인하였다. TBARS에 의한 LDL산화 억제효과 측정시, 생열귀나무 뿌리의 메탄올 추출물이 가장 좋은 효과를 나타내었다. 생열귀나무 뿌리의 에틸아세테이트 및 부탄을 분회물의 경우, $30\;{\mu}g/mL$ 첨가시 85.3% 및 93.2%까지 LDL 산화를 억제함을 확인하였다. 또한 $CHCl_3$ 분획물, EtOAc 분획물, 그리고 BuOH 분획물은 $30\;{\mu}g/mL$ 분획물 첨가시 $Cu^{2+}$에 의한 LDL 산화를 8시간까지 억제함을 확인하였다. $Cu^{2+}$에 의한 apoB carbonylation과 conjugate diene형성 억제효과 측정시, EtOAc 분획물과 BuOH 분회물이 가장 탁월하게 억제함을 보여주었다. 생열귀나무는 다량의 페놀성 화합물을 함유하고 있으며 항산화 활성이 매우 탁월하므로 새로운 기능성식품 소재로의 활용이 기대되는 약용식물자원이다.

Keywords

References

  1. Caslake MJ, Packard CJ, Shepherd J. Plasma triglyceride and LDL metabolism. Eur. J. Clin. Invest. 22: 96-104 (1992) https://doi.org/10.1111/j.1365-2362.1992.tb01942.x
  2. Henriksen T, Mahoney EM, Steinberg D. Enhanced macrophage degradation of biologically modified low density lipoprotein. Arteriosclerosis 3: 149-159 (1983) https://doi.org/10.1161/01.ATV.3.2.149
  3. Morel DW, Docorleto PE, Chisolm GM. Endothelial and smooth muscle alter low density lipoprotein in vitro by free radical oxidation. Atherosclerosis 4: 357-364 (1984)
  4. Bruckdorfer KR. Free radicals, lipid peroxidation and atherosclerosis. Curr. Opin. Lipidol. 1: 529-535 (1990) https://doi.org/10.1097/00041433-199012000-00008
  5. Steinbrecher U, Parthasarathy S, Leake DS, Witztum JL, Steinberg D. Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids. Proc. Natl. Acad. Sci. USA 83: 3883-3887 (1984)
  6. Esterbauer H, Streigel G, Puhl H, Oberreither S, Rotherneder M, El-Saadani M, Urgens G. The role of vitamin E and carotenoids in preventing oxidation of low density lipoproteins. Ann. N. Y. Acad. Sci. 570: 254-267 (1989) https://doi.org/10.1111/j.1749-6632.1989.tb14925.x
  7. Manigiapane H, Thomson J, Salter A, Brown S, Bell GD, White DA. The inhibition of the oxidation of low density lipoprotein by (+)-catechin, a naturally occurring flavonoid. Biochem. Pharmacol. 43: 445-450 (1992) https://doi.org/10.1016/0006-2952(92)90562-W
  8. De Whalley CV, Rankin SM, Hoult JRS, Jessup W, Leake DS. Flavonoids inhibit the oxidative modification of low density lipoproteins by macrophages. Biochem. Pharmacol. 39: 1743-1750 (1990) https://doi.org/10.1016/0006-2952(90)90120-A
  9. Machlin LJ, Bendich A. Free radical tissue damage: Protective role of antioxidant nutrients. FASEB J. 1: 441-445 (1987) https://doi.org/10.1096/fasebj.1.6.3315807
  10. Retsky KL, Frei B. Vitamin C prevents metal ion-dependent initiation and propagation of lipid peroxidation in human low-dinsity lipoprotein. Biochim. Biophys. Acta 1257: 279-287 (1995) https://doi.org/10.1016/0005-2760(95)00089-U
  11. Noguchi N, Gotoh N, Niki E. Dynamics of the oxidation of low density lipoprotein induced by free radicals. Biochim. Biophys. Acta 1168: 348-357 (1993) https://doi.org/10.1016/0005-2760(93)90192-C
  12. Yan LJ, Dray-Lefaix MT, Packer L. Ginko biloba extract(EGb 761) protects human low density lipoproteins against oxidative modification mediated by copper. Biochem. Biophy. Res. Com. 213: 360-366 (1995)
  13. Jeong J, Kim E, Hwangbo H, Ham S. Effects of Ligularia fischeri extracts on oxidation of low density lipoprotein. Korean J. Food Sci. Techol. 30: 1214-1221. (1998)
  14. Park CO, Kim KS, Ji YA, Ryu BH. Antioxidant activity of daidzin and puerarin toward oxidation of human low density lipoprotein. J. Korean Soc. Food Sci. Nutr. 26: 25-31 (1997)
  15. Park CO, Jin SH, Ryu BH. Antioxidant activity of green tea extracts toward human low density lipoprotein. Korean J. Food Technol. 28: 850-858 (1996)
  16. Yag KS, Jeon CM. Effect of Taraxacum coreanum Nakai on low density lipoprotein oxidation. Korean J. Pharmacogn. 27: 267-273 (1996)
  17. Shin KH, Chung HS, Cho SH. Vitamin contents in the fruits of Rosa davurica Pall. J. Med. Crop Sci. 3: 21-24 (1995)
  18. Shin KH, Lim SS, Lee SH, Seo JS, Yu SY, Park CH. Vitamin content in Rosa davurica Pall. J. Medicinal Crop Sci. 6: 6-10 (1998)
  19. Sa JH, Shin IC, Jeong KJ, Shim TH, Oh H, Park SK, Cheung E, Kim SN, Kim GG, Choi DS, Kwon YS, Kim CM. Catechin content and antioxidative effect from Rosa davurica Pall. Korean J. Pharmacogn. 33: 177-181 (2002)
  20. Kim JY, Maeng YS, Lee KY. Antioxidative effects of soybean extracts by using various solvents. Korean J. Food Sci. Technol. 27: 635-639 (1995)
  21. Seo YH, Kim IJ, Yie AS, Min HK. Electron donating ability and contents of phenolic compounds, tocopherols and carotenoids in waxy corn (Zea mays L.). Korean J. Food Sci. Technol. 31: 581-585 (1999)
  22. AOAC. Official Methods of Analysis, 15th ed. Association of Official Analytical Chemists, Arlington, VA, USA (1980)