Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Activity of Germinated Specialty Rices

발아 특수미의 항산화 활성

  • Kang, Mi-Young (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Sul-Yi (Department of Biological Science, Ajou University) ;
  • Koh, Hee-Jong (Department of Agronomy, Seoul National University) ;
  • Chin, Joong-Hyoun (Department of Agronomy, Seoul National University, Shinji Corp.) ;
  • Nam, Seok-Hyun (Department of Biological Science, Ajou University)
  • Published : 2004.08.31
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Abstract

Functionality changes by germination of giant embryonic rice and pigmented rice were evaluated with focusing on antioxidative activities of 70% ethanolic extracts. Overall, reducing power of giant embryonic rice and pigmented rice was higher than that of normal brown rice, and the germination of rices tend to enhance their reducing powers. In vitro and ex vivo experiments employing linoleic acid peroxidation and rabbit erythrocyte membrane peroxidation systems, respectively, revealed inhibitory effect on lipid peroxidation was highest in pigmented rice, followed by giant embryonic rice, and normal brown rice from high to low order. Superoxide radical-scavenging activity decreased in order of pigmented rice > giant embryonic rice > normal brown rice, and germination also enhanced their superoxide scavenging ability compared to non-germinated controls. Hydroxyl radical-scavenging ability was highest in pigmented rice, followed by giant embryonic rice, and normal brown rice. Despite marked enhancement in hydroxyl radical-scavenging ability of normal brown rice by germination, order of scavenging ability was not altered among germinated rices. Same trend as with in vitro ROS scavenging was observed for ex vivo scavenging potency on ROSs generated by TPA stimulation in HL-60 cells. Germination-associated differential increase in ROS scavenging ability of pigmented rice and giant embryonic rice, characterized by no induction of cytotoxicity, was observed.

발아에 의한 거대배아미와 유색미의 기능성 변화를 항산화 활성에 중심을 두고 조사하였다. 전반적으로 환원력은 일반현미보다 거대배아미와 유색미가 높았고, 발아처리는 환원력을 증가시키는 경향이 있었다. 지질과산화에 대한 억제효과를 조사한 결과, in vitro의 linoleic acid peroxidation system 및 ex vivo의 토끼 적혈구 막지질 과산화 system 모두에서 억제효과도 유색미가 가장 높았고, 그 다음이 거대배아미, 일반현미의 순서로 나타났다. Superoxide radical에 대한 소거활성은 유색미>거대배아미>일반현미의 순서로 낮아졌으며, 무발아 조건과 비교할 때 발아처리는 라디칼에 대한 소거활성을 증진시켰다. Hydroxyl radical 소거활성도 유색미가 가장 높았으며, 그 다음이 거대배아미, 일반현미의 순서였다. 발아에 의하여 일반현미의 hydroxyl radical 소거활성을 급격히 증가되었지만, 발아미간에 소거활성의 순위는 변하지 않았다. In vitro에서 조사된 ROS 소거활성과 같은 경향이 HL-60 세포에서 TPA로 자극으로 발생된 ROS에 대한 소거활성에서도 나타났다. 실험 결과, 유색미와 거대배아미에서는 발아와 연관되어 세포독성의 증가가 발견되지 않는 차별적인 ROS 소거활성의 증가 현상이 발견되었다.

Keywords

References

  1. Ramarathnam N, Osawa T, Namiki M, Kawakishi S. Chemical studies on novel antioxidants I. Isolation, fractionation and partial characterization. J. Agric. Food Chem. 36: 727-732 (1988)
  2. Ramarathnam N, Osawa T, Namiki M, Kawakishi S. Chemical studies on novel antioxidants II. Identification of isovitexin C-glycosyl flavonoid. J. Agric. Food Chem. 37: 316-319 (1989) https://doi.org/10.1021/jf00086a009
  3. Kang MY, Choi YH, Nam SH. Inhibitory mechanism of colored rice bran extract against mutagenicity induced by chemical mutagen Mitomycin C. Agri. Chem. Biotechnol. 39: 424-429 (1996)
  4. Nam SH, Kang MY. In vitro inhibitory effect of colored rice bran extracts carcinogenicity. Agri. Chem. Biotechnol. 40: 307-312 (1997)
  5. Choi SW, Nam SH, Choi HC. Antioxidative activity of ethanolic extracts of rice brans. Food Sci. Biotechnol. 5: 305-309 (1996)
  6. Juliano BO. Rice-Chemistry and Technology. AACC Press, London, UK. pp. 295-311 (1985)
  7. Cho BM, Yoon SK, Kim WJ. Changes in amino acid and fatty acids composition during germination of rapeseed. Korean J. Food Sci. Technol. 17: 371-376 (1985)
  8. Hsu D, Leung HK, Finney PL, Morad MM. Effect of germination on nutrative value and baking properties of dry peas, lentils and faba beans. J. Food Sci. 45: 87-91 (1980) https://doi.org/10.1111/j.1365-2621.1980.tb03877.x
  9. Choi KS, Kim ZU. Changes in lipid components during germination of mungbean. Korean J. Food Sci. Technol. 17: 271-275 (1985)
  10. Colmenarse DRAS, Bressani R. Effect of germination on the chemical composition and nutrative valus of amaranth grain. Cereal Chem. 67: 519-523 (1990)
  11. Lee MH, Son HS, Choi OK, Oh SK, Kwon TB. Changes in physico-chemical properties and mineral contents during buckwheat germination. Korean J. Food Nutr. 7: 267-273 (1994)
  12. Kim IS, Kwon TB, Oh SK. Study on the chemical change of general composition fatty acids and minerals of rapeseed during germination. Korean J. Food Sci. Technol. 17: 371-376 (1985)
  13. Ikeda K, Arioka K, Fujii S, Kusano T, Oku M. Effect on buckwheat protein quality of seed germination and changes in trypsin inhibitor content. Cereal Chem. 61: 236-240 (1984)
  14. Kim WJ, Kim NM, Sung HS. Effect of germinationon phytic acid and soluble minerals in soymilk. Korean J. Food Sci. Technol. 16: 358-362 (1984)
  15. Ahn B, Yang CB. Effects of soaking, germination, incubation and autoclaving on phytic acid in seed. Korean J. Food Sci. Technol. 17: 516-521 (1985)
  16. Lee MH, Shin JC. New techniques for the cultivation of quality rice. pp. 239-263. In: Conference on Rediscovering Korea Rice and Development Direction. Korean Society of Rice Research, Seoul, Korea (1999)
  17. Nakagawa K, Onota A. Accumulation of $\gamma-aminobutyric$acid (GABA) in the rice germ. Food Proc. 31: 43-46 (1996)
  18. Oyaizu M. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 301-315 (1986)
  19. Osawa T, Namiki M. A novel type antioxidant isolated from leaf wax of Eukalyptus leaves. Agric. Biol. Chem. 45: 735-740 (1981) https://doi.org/10.1271/bbb1961.45.735
  20. Ames BN, Cathcart R, Schwiers E, Hochstein P. Uric acid provide an antioxidant defense in human aganist oxidant-and radicalcaused aging and cancer: A hypothesis. Proc. Natl. Acad. Sci. USA 78: 6858-6862 (1981) https://doi.org/10.1073/pnas.78.11.6858
  21. Tsuda T, Horio F, Osawa T. Dietary cyanidin 3-O-$\beta$-D-glucoside increases ex vivo oxidation resistance of serum in rats. Lipids 33: 583-588 (1998) https://doi.org/10.1007/s11745-998-0243-5
  22. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Chem. 72: 248-254 (1976)
  23. Mitsuta K, Mizuta Y, Kohno M, Hiramatsu M. The application of ESR spin trapping technique to the evaluation of SOD-like activity of biological substances. Bull. Chem. Soc. Jpn. 63: 187-190 (1990) https://doi.org/10.1246/bcsj.63.187
  24. Noro T, Noro K, Miyase T, Koyanagi M, Umehara K, Ueno A, Fukushima S. Inhibition of xanthine oxidase by anthraquinones. Chem. Pharm. Bull. 35: 4314-4316 (1987) https://doi.org/10.1248/cpb.35.4314
  25. Fenton HJH. Oxidation of tartaric acid in presence of iron. J. Chem. Soc. 65: 899-910 (1894) https://doi.org/10.1039/ct8946500899
  26. Rosen GM, Rauckman EJ. Spin trapping of free radicals during hepatic microsomal lipid peroxidation. Proc. Natl. Acad. Sci. USA 78: 7346-7349 (1981) https://doi.org/10.1073/pnas.78.12.7346
  27. Carter P. Spectrometric determination of serum ion at the submicrogram level with a new reagent (Ferrozine). Anal. Chem. 40: 450-458 (1971)
  28. Lin JK, Chen PC, Ho CT, Lin-Shiau SY. Inhibition of xanthine oxidase and suppression of intracellular reactive oxygen species in HL-60 cells by theaflavin-3,3'-digallate, (-)-epigallocatechin-3- galate, and propyl gallate. J. Agric. Food Chem. 48: 2736-2743 (2000) https://doi.org/10.1021/jf000066d
  29. Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimualtion. J. Immunol. 130: 1910-1917 (1983)
  30. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assay. J. Immunol. Meth. 65: 55-63 (1983) https://doi.org/10.1016/0022-1759(83)90303-4
  31. Choi SW, Kang WW, Osawa T. Isolation and identification of anthocyanin pigments in black rice. Foods Biotechnol. 3: 131-135 (1994)
  32. Tsuda T, Watanabe M, Ohshima K, Norinobu S, Kawakishi S, Choi SW, Osawa T. Antioxidative activity of the anthocyanin pigments cyanidin 3-O-$\beta$-D-glucoside and cyanidin. J. Agric. Food Chem. 42: 2407-2411 (1994) https://doi.org/10.1021/jf00047a009
  33. Yamauchi R, Miyate N, Kato K, Ueno Y. Reaction of $\alpha$-tocopherol with alkyl and alkylperoxyl radicals of methyl linoleate. Lipids 28: 201-206 (1993) https://doi.org/10.1007/BF02536640
  34. Ames BN. Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases. Science 221: 1256-1264 (1983) https://doi.org/10.1126/science.6351251
  35. Ishihara K, Hirano T. IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine Growth Factor Rev. 13: 357-368 (2002) https://doi.org/10.1016/S1359-6101(02)00027-8