Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Identification of Pratensein with Antimicrobial Activity from the Peanut Shells

땅콩껍질에서 항미생물 활성을 지닌 pratensein의 분리 및 동정

  • Wee, Ji-Hyang (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Keun-Hyung (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
  • 위지향 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 문제학 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 박근형 (전남대학교 식품공학과 및 농업과학기술연구소)
  • Published : 2004.08.31
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Abstract

Natural antimicrobial substance from peanut (Arachis hypogaea) shells was isolated and structurally elucidated. Peanut shells were extracted with methanol (MeOH) and concentrated in vacuo, MeOH extract was solvent-fractionated with ethyl acetate (EtOAc) and various buffer to obtain EtOAc acidic, neutral, and phenolic fractions. EtOAc neutral fraction, which showed antimicrobial activity, was purified through silica gel adsorption column, Sephadex LH-20 column, ODS column, and high performance liquid chromatographies, and its active substance was isolated and identified as pratensein (3',5,7-trihydroxy-4'-methoxyisoflavone) by spectroscopic methods of proton-nuclear magnetic resonance, mass spectrometry, and nuclear overhauser enhancement spectroscopy.

땅콩껍질에 함유된 항미생물 활성물질의 탐색을 위해 땅콩껍질을 MeOH로 추출하고 이 MeOH 추출물을 용매분획하여 EtOAc 중성획분을 얻었다. 이 획분에 함유된 항미생물 활성물질을 silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography로 정제한 다음 HPLC를 이용하여 1종의 화합물을 단리하였다. $^1H-NMR$, MS 및 NOESY 분석에 의해 땅콩껍질로부터 분리된 항미생물 활성물질은 pratensein으로 동정되었다.

Keywords

References

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