Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Activity of the Extracts from Browned Oak Mushroom (Lentinus edodes) with Unmarketable Quality

시장성이 없는 품질의 갈변 표고버섯(Lentinus edodes) 추출물의 항산화 활성

  • Kang, Mi-Young (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Sul-Yi (Department of Biological Science, Ajou University) ;
  • Yun, Hye-Jung (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Nam, Seok-Hyun (Department of Biological Science, Ajou University)
  • Published : 2004.08.31
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Abstract

Physiological functionality of browned oak mushroom was evaluated with focusing its electron-donating ability to DPPH radicals, scavenging ability to superoxide radicals and hydroxyl radicals, and inhibitory activity on lipid peroxidation. The results showed that overall antioxidative activities of browned oak mushroom were superior to those of raw oak mushroom with marketable quality, implying possible involvement of resultant browning reaction products in an increment of antioxidativity. The increased radical-scavenging ability was suggested to mainly be exerted by direct quenching of both superoxide and hydroxyl radicals, not by inhibition of xanthine oxidase activity and $Fe^{2+}$ chelation, respectively. Collectively, these results indicate a possible use of unmarketable browned mushroom as a material for manufacturing various processed functional foods.

갈변된 표고버섯을 건강기능성 식품산업의 소재로 활용할 수 있는 가능성을 탐색하는 일환으로서 갈변된 표고버섯의 생리활성을 항산화 활성을 지표로 평가하였다. 본 연구에서는 DPPH 라디칼에 대한 전자공여능과 superoxide radical 및 hydroxyl radical에 대한 소거활성, 그리고 linoleic arid 자동산화반응에 대한 억제활성을 조사하여 각 추출물의 항산화 활성을 평가하였다. 그 결과, 갈변 표고버섯의 전자공여능은 생 표고버섯보다 추출방법에 따라 2-4배 정도 높았지만, 건조 표고버섯보다는 높거나 비슷한 수준을 보였다. Superoxide radical에 대한 소거활성도 DPPH에 대한 전자공여능과 유사한 경향을 보였는데, 갈변 표고버섯의 소거활성은 생 표고버섯보다 2배 정도 높았다. 또한 이와 같은 갈변화에 따른 superoxide radical 소거활성의 증가는 직접적인 radical 소거작용의 결과일 가능성이 제시되었다. Hydroxyl radical 소거활성의 경우, 추출방법에 관계없이 소거활성은 갈변 표고버섯 > 건조 표고버섯 > 생 표고버섯의 순서로 낮아졌으며, superoxide radical 소거작용과 마찬가지로 시료가 직접적으로 hydroxyl radical을 소거했을 가능성이 암시되었다. Linoleic acid 자동산화반응을 이용한 지질과산화에 대한 억제활성을 측정한 결과, 전반적으로 70% 에탄올추출물의 활성이 열수추출물보다 높았고, 억제활성도 갈변 표고버섯 > 건조 표고버섯 > 생 표고버섯의 순서로 낮아지는 경향을 보였다. 이상의 결과는 상품성을 상실한 갈변 표고버섯이 건강기능성을 지향한 다양한 가공식품의 개발을 위한 적절한 소재임을 보여주었다.

Keywords

References

  1. Aoyaki Y, Sugahara T. Studies on the rehydration of dried Shiitake mushroom. J. Food Sci. Technol. 33: 224-228 (1986)
  2. Minamida T, Habu T, Ogata K. Effect of storage temperature on keeping freshness of mushroom after harvest. J. Food Sci. Technol. 27: 281-286 (1980) https://doi.org/10.3136/nskkk1962.27.6_281
  3. Yamashita IK, Takahashi T, Shimona T, Kikuchi K, Shibata S. Changes in some chemical characteristics in Shiitake mushroom during modified atmosphere packaging storage and on exposure to air. J. Food Sci. Technol. 34: 834-840 (1987) https://doi.org/10.3136/nskkk1962.34.12_834
  4. Zixin A. Studies on retaining freshness and preventing discoloration of mushrooms. Food Ferment. Ind. 5: 32-37 (1982)
  5. Lee SE, Kim DM, Kim KH. Changes in quality of Shiitake Mushroom (Lentinus edodes) during modified atmosphere (MA) storage. J. Korean Soc. Food Nutr. 20: 133-138 (1991)
  6. Yen CG, Chen HY. Antioxidative activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43: 27-32 (1995) https://doi.org/10.1021/jf00049a007
  7. Mitsuda K, Mizuta Y, Kohno M, Hiramatsu M. The application of ESR spin-trapping technique to the evaluation of SOD-like activity of biological substances. Bull. Chem. Soc. Jpn. 63: 187-190 (1990) https://doi.org/10.1246/bcsj.63.187
  8. Noro T, Noro K, Miyase T, Kuroyanagi M, Umehara K, Ueno A, Fukushima S. Inhibition of xanthine oxidase by anthraquinones. Chem. Pharm. Bull. 35: 4314-4316 (1987) https://doi.org/10.1248/cpb.35.4314
  9. Bray TM, Levy MA. Model and Methods in Cell Signaling and Gene Expression. OICA International, London, UK. pp. 1-14 (2000)
  10. Rosen GM, Rauckman EJ. Spin trapping of free radicals during hepatic microsomal lipid peroxidation. Proc. Natl. Acad. Sci. USA 78: 7346-7349 (1981) https://doi.org/10.1073/pnas.78.12.7346
  11. Carter P. Spectrometric determination of serum ion at the submicrogram level with a new reagent (Ferrozine). Anal. Chem. 40: 450-458 (1971)
  12. Osawa T, Namiki MA. Novel type antioxidant isolated from leaf wax of Eukalyptus leaves. Agric. Biol. Chem. 45: 735-740 (1981) https://doi.org/10.1271/bbb1961.45.735
  13. Lee MH, Son HS, Choi OK, Oh SK, Kwon TB. Changes in physico-chemical properties and mineral contents during buckweat germination. Korean J. Food Nutr. 7: 267-273 (1994)
  14. Droge W. Free radical in the physiological control of cell function. Physiol. Rev. 82: 47-95 (2002) https://doi.org/10.1152/physrev.00018.2001
  15. Deliconstantinos G, Villiotou V, Stavrides JC. Alteration of nitric oxide synthase and xanthine oxidase activities of human keratinocytes by ultraviolet B radiation. Biochem. Pharmacol. 51: 1727-1738 (1997) https://doi.org/10.1016/0006-2952(96)00110-4
  16. Heyneman RA. Subcellular localization and properties of the NAD(P)H oxidase from equine polymorphonuclear leukocytes. Enzyme 29: 198-207 (1983) https://doi.org/10.1159/000469633
  17. Goldstein M, Kaplan HB, Edelson HS, Weissman G. Ceruloplastin: an acute phase reactant that scavenges oxygen-derived free radicals. Ann. N.Y. Acad. Sci. 389: 368-379 (1982) https://doi.org/10.1111/j.1749-6632.1982.tb22150.x
  18. Tsuchihashi H, Kigoshi M, Iwatsuki M, Niki E. Action of β-carotene as an antioxidant against lipid peroxidation. Arch. Biochem. Biophys. 323: 137-147 (1995) https://doi.org/10.1006/abbi.1995.0019
  19. Lindenmeier M, Faist V, Hofmann T. Structural and functional characterization of pronyl-lysine, a novel protein modification in bread crust melanoidins showing in vitro antioxidative and phase I/II enzyme modulating activity. J. Agric. Food Chem. 50: 6997-7006 (2002) https://doi.org/10.1021/jf020618n