Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Quantitative Analysis of Genetically Modified Soybean in Processed Foods Using Real-time PCR

실시간중합효소연쇄반응을 이용한 유전자변형 콩 가공식품의 정량분석

  • Min, Dong-Myung (National Agricultural Products Quality Management Service, Ministry of Agriculture, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Myo-Young (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Jung, Soon-Il (National Agricultural Products Quality Management Service, Ministry of Agriculture) ;
  • Heo, Mun-Seok (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Jin-Kuk (National Agricultural Products Quality Management Service, Ministry of Agriculture) ;
  • Kim, Hae-Yeong (Department of Food Science and Biotechnology, Kyung Hee University)
  • 민동명 (국립농산물품질관리원 시험연구소, 경희대학교 식품생명공학과) ;
  • 김묘영 (경희대학교 식품생명공학과) ;
  • 정순일 (국립농산물품질관리원 시험연구소) ;
  • 허문석 (경희대학교 식품생명공학과) ;
  • 김진국 (국립농산물품질관리원 시험연구소) ;
  • 김해영 (경희대학교 식품생명공학과)
  • Published : 2004.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Qualitative and quantitative PCR methods were performed to examine detection and quantitation of epsps inserted into genetically modified soybean (GMS) in processed foods, soy milk, tofu, and biji (soybean curd residue). Using PCR amplification to produce two (121 and 330 bp) epsps in GMS, detection limits of GMS in soy milk, tofu, and biji containing 0.01% GMS were measured. For quantitative detection, test samples containing 1, 3, and 5% GMS were measured by real-time PCR method. Results show real-time PCR method is applicable to detect GMS quantitatively in processed foods.

콩 가공식품인 두유, 두부, 비지에서 유전자변형 콩에 삽입된 epsps 유전자의 검출과 정량을 위해 정성과 정량 PCR 방법을 수행하였다. 두유, 두부, 비지에서 유전자변형 콩의 검출은 삽입된 epsps 유전자의 증폭이 크기가 121bp와 330bp가 생성될 수 있는 두 종류의 primer들을 이용하여 0.01%까지 확인하였다. 정량방법은 1, 3, 5%의 유전자변형 콩이 포함된 시료들을 실시간 PCR을 사용하여 유의성 있는 결과를 얻었다. 이러한 결과들은 실시간 PCR 방법을 사용하여 가공식품 내에서 정량적으로 유전자변형 콩을 정량하는데 적용될 수 있음을 보였다.

Keywords

References

  1. James C. Preview: Global Status of Commercialized Transgenic Crops: ISAAA Briefs No. 30. ISAAA, Ithaca, NY, USA (2003)
  2. Auer CA. Tracking genes from seed to supermarket: techniques and trends. Trends Plant Sci. 8: 591-597 (2003) https://doi.org/10.1016/j.tplants.2003.10.010
  3. Ahmed FE. Detection of genetically modified organisms in foods. Trends Biotechnol. 5: 215-223 (2002)
  4. Korea Ministry of Agriculture and Forestry. Regulation Concerning the Compulsory Labeling of Genetically Modified Agricultural Organisms. Korea Ministry of Agriculture and Forestry, Seoul, Korea (2000)
  5. Korea Food and Drug Adminstration. Regulation Concerning the Compulsory Labeling of Foodstuffs and Food Ingredients Produced from Genetically Modified Organisms. Korea Food and Drug Adminstration, Seoul, Korea (2001)
  6. Kim HJ, Park SH, Kim HY. Study for detection of glyphosate tolerant soybean using PCR. Korean J. Food Sci. Technol. 33: 521-524 (2001)
  7. Kim YM, Sohn SH, Jeong SI, Yoon MS, Kim TS, Park YH. Detection methods for genetically modified soybeans. J. Korean Soc. Agric. Chem. Biotechnol. 45: 185-189 (2002)
  8. Jung S. Studies on the detection of genetically modified organism using polymerase chain reaction. MS thesis. Kongju University, Kongju, Korea (2004)
  9. Kim JH. Studies on the polymerase chain reaction for the rapid detection of genetically modified foods. MS thesis. Kyunghee University, Suwon, Korea (2003)
  10. Kuribara H, Shindo Y, Matsuoka T, Takubo K, Futo S, Aoki N, Hirao T, Akiyama H, Goda Y, Toyoda M, Hino A. Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 85: 1077-1089 (2002)
  11. Shindo Y, Kuribara H, Matsuoka T, Futo S, Saqada C, Shono J, Akiyama H, Goda Y, Toyoda M, Hino A. Validation of real-time PCR analysis for line-specific quantitation of genetically modified maize and soybean using new reference molecules. J. AOAC Int. 85: 1119-1126 (2002)
  12. Kim MY, Kim JH, Kim HJ, Park SH, Woo GJ. Kim HY. Monitering of genetically modified soybean and processed foods in Korean market using PCR. J. Korean Soc. Agric. Chem. Biotechnol. 46: 344-347 (2003)