Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Green Tea, Buckwheat and Grape Leaves Extracts on Lipid Metabolism, Antioxidative Capacity, and Antithrombotic Activity in Rats Fed High Cholesterol Diets

녹차, 메밀, 포도잎 추출물이 고콜레스테롤 급여 흰쥐의 지질대사, TBARS 및 혈액응고에 미치는 영향

  • Published : 2004.12.31
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Abstract

Effects of green tea, buckwheat, and grape leaf extracts on factors related to blood circulation were studied using rats fed high-cholesterol diet for 4 weeks. Rats were randomly divided into five groups, and plant extracts were orally administered. Green tea extract increased bleeding time in rat tails, suggesting it could prevent platelet aggregation. Administration of green tea, buckwheat, and grape leaf extracts decreased total cholesterol level in liver. Grape leaf extracts decreased thiobarbituric acid-reactive substances in plasma, whereas buckwheat and grape leaf extracts decreased the substances in liver. These results showed extracts of green tea, buckwheat, and grape leaf were effective for improving lipid composition in blood and liver and inhibiting lipid peroxidation in animal tissue, suggesting they may have potential to prevent cardiovascular diseases.

본 연구에서는 Sprague Dawley 종 수컷 흰쥐에게 콜레스테롤이 1% 함유된 사료를 급여하여 고지혈증을 유발시키면서 녹차추출물, 메밀추출물, 포도잎추출물 추출물을 500 mg/kg B.W.의 농도로 4주간 매일 같은 시각에 경구투여하여 흰쥐의 혈액과 간장 내의 지질조성과 혈액응고기전 및 체내 지질과산화에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. 전군에서 식이섭취량과 체중증가량 및 식이효율은 유의한 차이가 없어서 실험식이가 정상적인 성장에 영향을 미치지 않았다. 녹차추출물투여군은 고콜레스테롤 급여 대조군에 비해서 흰쥐의 꼬리에서의 출혈시간을 증가시켜서 항혈전효과가 있었다. 고콜레스테롤 급여로 인해 흰쥐에게 고지혈증이 유발되었으며 실험 시료를 경구투여하는데 따른 혈액중의 지질대사 개선효과는 없었다. 고콜레스테롤 급여로 흰쥐의 간장은 비대해지고 간장내 중성지방과 총콜레스테롤 함량도 현저히 증가하였으며 녹차추출물, 메밀추출물, 포도잎추출물은 간장 내 총콜레스테롤 함량을 감소시켜 간장 내 지질대사의 개선효과가 있었다. 고콜레스테롤의 급여는 혈장 내의 지질과산화물의 함량을 증가시키며 포도잎추출물은 체내 지질과산화를 억제시키는 효과가 있었으며 간에서는 메밀과 포도잎추출물이 지질과산화를 억제하였다.

Keywords

References

  1. NSOK. Annual Report on the Cause of Death Statistics. National Statistical Office of Korea, Seoul, Korea (2003)
  2. NSOK. The Expectancy of Future Population. National Statistical Office of Korea (2000)
  3. Lee HK. The nutrition ploblems of Koreans. Korean J. Nutr. 29: 381-383 (1996)
  4. Stephanie B, Sabrina SI, Bernard F, Guylene P, Alain P, Laurence B. Effect of ischemia on TBARS and lactate production in several cerebral regions of anaesthetised and awake rats. Life Sci. 74: 3103-3113 (2004) https://doi.org/10.1016/j.lfs.2003.11.013
  5. Endres M. Ischemia and stroke. Vol. 513, pp. 455-468. In: Advances in Experimental Medicine and Biology. Dirnagl U. Plenum Press, Inc., NY, USA (2002)
  6. Ikeda K, Negishi H, Yamori Y. Antioxidant nutrients and hypoxia/ischemia brain injury in rodents. Toxicology 189: 55-61 (2003) https://doi.org/10.1016/S0300-483X(03)00152-5
  7. Shin DH. The research and prospect of natural antioxidants. Bull. Food Technol. 8: 28-33 (1995)
  8. Kim YJ. The protect the living organ from free radicals and the failure of protection: age-related disease. Bull. Food Technol. 10: 4-26 (1997)
  9. Buring JE, Henekens CH. Antioxidant vitamins and cardiovascular disease. Nutr. Rev. 55: S53-S60 (1997) https://doi.org/10.1111/j.1753-4887.1997.tb06103.x
  10. Jacques PF, Halpner AD, Blumberg JB. Influence of combined antioxidant nutrient intake on their plasma concentrations in an elderly population. Am. J. Clin. Nutr. 62: 1228-1233 (1995) https://doi.org/10.1093/ajcn/62.6.1228
  11. Oh SW, Kwon EK, Kim KY. Screening of angiotensin converting enzyme inhibitory activity in several natural products (abstract no P7-17). In: 69th Symposium of Food Science and Technology. October 24-26, Hotel Tirol in Muju resort, Muju, Korea. The Korean Society of Food Science and Technology, Seoul, Korea (2002)
  12. Kwon EK, Oh SW, Lee CH, Han D. Screening of HMG-CoA reductase inhibitory activity in several natural products (abstract no P7-32). In: 70th Nutrigenomics Symposium: Recent trends in food science and technology. June 26-28, Gyeongju TEMF Hotel, Gyeongju, Korea. The Korean Society of Food Science and Technology (2003)
  13. Friedewald WT, Levy RI, Fredrickson DS. Estimation of concentration of low density lipoprotein cholesterol in plasma without use of ultracentrifuge. Clin. Chem. 18: 499-502 (1972)
  14. Rosenfeld L. Lipoprotein analysis. Arch. Pathol. Lab. Med. 113: 1101-1110 (1989)
  15. Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  16. Hornstra G, Christ-Hazelhof E, Haddeman E, Hoor FT, Nugteren DH. Fish oil feeding lowers thromboxane-and prostacylin production by rat platelets and aorta and does not result in the formation of prostaglandin I3. Prostaglandins 21: 727-738 (1981) https://doi.org/10.1016/0090-6980(81)90230-6
  17. Kim ES, Kim MK. Effect of dried leaf powers and ethanol extracts of persimmon, green tea and pine needle on lipid metabolism and antioxidative capacity in rats. J. Korean Soc. Food Sci. Nutr. 32: 337-352 (1999)
  18. Yun YP, Kang WS, Lee MY. The antithrombotic effects of green tea catechins. J. Food Hyg. Safety 11: 77-82 (1996)
  19. Kannel, WB. Risk factors in hypertensions. J. Cardiovasc. Pharmacol. 13: S4-S10 (1989) https://doi.org/10.1097/00005344-198900131-00003
  20. Oh HS, Park YH, Kim JH. Isoflavone contents, antioxidative and fibrinolic activities of some commercial cooking-with-rice soybeans. Korean J. Food Sci. Technol. 34: 498-504 (2002)
  21. Lee E, Choi MY. Effects of pine needle on lipid composition and TBARS in rat fed high cholesterol. Korean J. Food Sci. Technol. 32: 1186-1190 (2000)
  22. Kim SY, Lee WC, Kim HB, Kim AJ, Kim SK. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol- induced hyperlipidemia rats. J. Korean Soc. Food Sci. Nutr. 27: 1217-1222 (1998)
  23. Han CK, Lee BH, Song KS, Lee NH, Yoon CS. Effects of antihypertensive diets mainly consisting of buckwheat, potato and perilla seed on spontaneously hypertensive rats. Korean J. Nutr. 29: 1087-1095 (1996)
  24. Wooller LA, Spady DK, Dietschy JM. Saturated and unsaturated fatty acids independently regulate low density lipoprotein receptor activity and production rate. J. Lipid Res. 33: 77-88 (1992)
  25. Holme I. An analysis of randomized trials evaluating the effect of cholesterol reduction on total mortality and coronary heart disease incidence. Circulation 82: 1916-1924 (1990) https://doi.org/10.1161/01.CIR.82.6.1916
  26. Spady DK, Dietschy JM. Interaction of dietary cholesterol and triglyceride in the regulation of hepatic low density lipoprotein transport in the hamster. J. Clin. Invest. 81: 300-309 (1988) https://doi.org/10.1172/JCI113321
  27. Liu CH, Huang MT, Huang PC. Sources of triglycerol accumulation in liver of rats fed a cholesterol supplemented diets. Lipids 30: 527-531 (1995) https://doi.org/10.1007/BF02537027
  28. Chi MS, Koh ET, Stewart TJ. Effects of garlic on lipid metabolism in rats fed cholesterol of lard. J. Nutr. 112: 241-248 (1982) https://doi.org/10.1093/jn/112.2.241
  29. Kang JA, Kang JS. Effect of garlic and onion on plasma and liver cholesterol and triacylglycerol and platelet aggregation in rats fed basal or cholesterol supplemented diets. Korean J. Nutr. 30: 132-138 (1997)
  30. Fridovich I. Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247: 1-15 (1986) https://doi.org/10.1016/0003-9861(86)90526-6
  31. Miquel J, Quintailha AT. Historical introduction to free radical and antioxidant biomedical research. Vol. 1, pp. 3-16. In: CRC Handbook of Free Radical and Antioxidants in Biomedicine. Weber H (ed). CRC Press, Inc., Boca Raton, FL, USA (1989)
  32. Babiy AV, Gebicki JM, Sullivan DR. Vitamin E content and low density oxidizability induced by free radicals. Atherosclerosis 81: 175-182 (1990) https://doi.org/10.1016/0021-9150(90)90064-P
  33. Buege JA, Aust SD. Microsomal lipid peroxidation. Vol. 52, pp. 302-310, In: Methods in Enzymology. Sidney F, Lester P (eds). Academic Press, Inc., NY, USA (1978) https://doi.org/10.1016/S0076-6879(78)52032-6