Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Acetylcholinesterase Inhibitory Activity and Protective Effect against Cytotoxicity of Perilla Seed Methanol Extract

들깨 메탄올 추출물의 acetylcholinesterase 억제활성 및 세포독성 보호효과

  • Choi, Won-Hee (Food Function Research Division, Korea Food Research Institute) ;
  • Um, Min-Young (Food Function Research Division, Korea Food Research Institute) ;
  • Ahn, Ji-Yun (Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Sung-Ran (Food Function Research Division, Korea Food Research Institute) ;
  • Kang, Myung-Hwa (Department of Food Science and Nutrition, Hoseo University) ;
  • Ha, Tae-Youl (Food Function Research Division, Korea Food Research Institute)
  • 최원희 (한국식품연구원 식품기능연구본부) ;
  • 엄민영 (한국식품연구원 식품기능연구본부) ;
  • 안지윤 (한국식품연구원 식품기능연구본부) ;
  • 김성란 (한국식품연구원 식품기능연구본부) ;
  • 강명화 (호서대학교 식품영양학과) ;
  • 하태열 (한국식품연구원 식품기능연구본부)
  • Published : 2004.12.31
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Abstract

Acetylcholinesterase inhibitory activity and protective effect against cytotoxicity of PC 12 cell induced by beta-amyloid protein and glutamate were examined in perilla seed methanol extract and its solvent fractions. Methanol extract of perilla seed showed dose-dependent acetylcholinesterase inhibitory activity, with n-butanol fraction showing strongest activity. Perilla seed methanol extract also decreased glutamate- and ${\beta}-amyloid$ protein $(A{\beta})-induced$ cytotoxicities of PC 12 cells dose-dependently. Formation of TBARS induced by $FeSO_{4^-}H_2O_2$ in rat brain was significantly reduced by perilla seed methanol extract, with strongest protective activity formation of TBARS shown in n-butanol fraction. Results suggest perilla seed methanol extract may attenuate actylcholinesterase activity and cytotoxicity induced by glutamate and ${\beta}-amyloid$ protein through suppression of oxidative stress.

들깨 메탄올 추출물이 acetylcholinesterase(AChE)의 활성 및 PC 12 cell 세포사멸에 미치는 영향을 검토하였다. AChE에 대한 억제환성은 들깨 메탄을 추출물의 농도가 높을수록 유의적으로 높았으며 추출물의 분획물 중에서는 n-butanol층이 가장 높은 억제율을 나타내었다. L-glutamate 또는 ${\beta}-amyloid$ protein $(A{\beta})$으로 유도된 PC 12 cell에 대한 세포사멸 억제효과도 추출물의 농도가 높을수록 유의적으로 증가하였다. 또한 rat brain에 $FeSO_{4^-}H_2O_2$로 산화적 스트레스를 유발시켜 추출물의 TBARS 생성 억제활성을 조사한 결과 들깨 메탄을 추출물은 농도가 높아질수록 산화적 스트레스에 대한 억제활성이 증가하였으며 분획물 중에서는 n-butanol층에서 가장 높은 억제활성을 나타내었다. 이상의 결과들로 미루어 볼 때 들깨 메탄을 추출물은 AChE 활성을 억제하고 glutamate 또는 $A{\beta}$에 의하여 유도된 PC12 cell의 세포사멸을 억제하며 이러한 효과는 들깨 메탄을 추출물의 항산화력에 기인할 수도 있을 것으로 사료되었다.

Keywords

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