Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Reduction of Photodamage by TopicaI Application of a Novel Anti-Wrinkle Agent Containing Growth Factors

  • Published : 2004.09.01
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Abstract

DA-3711 is a novel anti-wrinkle agent containing growth factors derived from culture medium of artificial human skin. Photoprotective effect by DA-3711 against chronic UVB (ultraviolet B)-induced skin damage was investigated in hairless mice model. Methods: After hairless mice were irradiated to induce photodamage for 8 weeks with UVB, grouped mice were treated topically once a day with lotion base, DA-3711 (30% or l5%), Cylasphere retinol$^{\circed{R}}$ (2500 I.U.), NouriCel$^{\circed{R}}$ along with concomitant exposure to UVB for further 8 weeks. Then mice were sacrificed to assess photodamage-protective effect by replica analysis, biochemistry and histology. DA-3711 of 30% lotion significantly reduced UVB radiation-induced wrinkling, histological alterations and increased collagen contents. Whereas DA-3711 of l5% lotion and NouriCel$^{\circed{R}}$ treatment showed a partial protective effect on skin wrinkle, epidermal and dermal thickness, and collagen content, Cylasphere retinol$^{\circed{R}}$ showed no protective effects. These results demonstrate that topical application of DA-3711 can alleviate UVB-induced photodamage and potentially be used for reduction of UVB-induced photodamage.

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References

  1. Bernstein, E.F., Chen, Y.Q. and Tarnai K. (1994). Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J. Invest. Dermatol. 103, 182-186 https://doi.org/10.1111/1523-1747.ep12392693
  2. Bissett, D.L., Hannon, D.P. and Orr, T.Y. (1987). An animal model of solar-aged skin: histological, physical, and visible changes in UV-irradiated hairless mouse skin. Photochem. Photobiol. 46, 367-378 https://doi.org/10.1111/j.1751-1097.1987.tb04783.x
  3. Bissett, D.L., Chatterjee, R and Hannon, D.P. (1990). Photoprotective effect of superoxide-scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse. Photodermatol. Photoimmunol. Photome. 7, 56-62.
  4. Bryce, G.F., Bogdan, N.J. and Brown, C.C. (1988). Retinoic acids promote the repair of the dermal damage and the effacement of wrinkles in the UVB-irradiated hairless mouse. J. Invest. Dermatol. 91, 175-180 https://doi.org/10.1111/1523-1747.ep12464445
  5. Chatterjee, R, Benzinger, M.J., Ritter, J.L. and Bissett, D.L. (1990). Chronic ultraviolet B radiation-induced biochemical changes in the skin of hairless mice. Photochem. Photobiol. 51,91-97. https://doi.org/10.1111/j.1751-1097.1990.tb01688.x
  6. Chen, S., Kiss, I. and Tramposch, K.M. (1992). Effects of alltrans retinoic acid on UVB-irradiated hairless mouse skin. J. Invest. Dermatol. 98, 248-254 https://doi.org/10.1111/1523-1747.ep12556066
  7. Circolo, A, Welgus, H.G., Pierce, G.P., Kramer, J. and Strunk, R.C. (1991). Differential regulation of the expression of proteinases/ antiproteinases in fibroblasts; Effects of interleukin-l and platelet-derived growth factor. J. Bioi. Chem. 266, 12283-12288
  8. Corcuff, P. and Leveque, J.L. (1993). Age related changes in skin microrelief measured by image analysis. In Aging skin. Edited by Leveque, J.L. and Agache, P.G. Marcel Dekker, New York, pp.179-197
  9. Edwards, D.R, Murphy, G. and Reynolds, J.J. (1987). Transforming growth factor beta modulates the expression of collagenase and metalloproteinase inhibitor. EMBO. J. 6, 1899-1904
  10. Farkas, B., Magyarlaki, M., Csete, B., Nemeth, J., Rabloczky, G., Bernath, S., Literati Nagy, P. and Sumegi, B. (2002). Reduction of acute photodamage in skin by topical application of a novel PARP inhibitor. Biochem. Pharmacol. 63,921-932 https://doi.org/10.1016/S0006-2952(01)00929-7
  11. Frances, C. and Robert, L. (1984). Elastin and elastic fibers in normal and pathologic skin. Int. J. Dermatol. 23, 166-179 https://doi.org/10.1111/j.1365-4362.1984.tb04506.x
  12. Gilchrest, B.A. (1989). Skin aging and photoaging: an overview. J. Am. Acad. Dermatol. 21,610-613 https://doi.org/10.1016/S0190-9622(89)70227-9
  13. Griffiths, C.E., Russman, A.N., Majmudar, G., Singer, R.S., Hamilton, T.A and Voorhees JJ. (1993). Restoration of collagen formation in photodamaged human skin by tretinoin (retinoic acid). New. Engl. J. Med. 329, 530-535 https://doi.org/10.1056/NEJM199308193290803
  14. Kligman, L.H., Duo, C.H. and Kligman, A.M. (1984). Topical retinoic acid enhances the repair of ultraviolet damaged dermal connective tissue. Connect. Tissue. Res. 12, 139-150 https://doi.org/10.3109/03008208408992779
  15. Kligman, L.H. (1986). Photoaging, manifestation, prevention, and treatment. Dermatol. Clin. 4,517-528
  16. Kligman, L.H. (1987). Full spectrum solar radiation as a cause of dermal photodamage; UVB to infrared. Acta. Derm. Venereol. Stockholm. 134, 53-61
  17. Kligman, L.H., Gebre, M., Alper, R and Kefalides, N.A. (1989). Collagen metabolism in ultraviolet irradiated hairless mouse skin and its correlation to histologic observations. J. Invest. Dermatol. 93, 210-214 https://doi.org/10.1111/1523-1747.ep12277573
  18. Koshiishi, I., Horikoshi, E., Mitani, H. and Imanari, T. (1999). Quantitative alterations of hyaluronan and dermatan sulfate in the hairless mouse dorsal skin exposed to chronic UV irradiation. Biochim. Biophys. Acta. 1428, 327-333. https://doi.org/10.1016/S0304-4165(99)00081-1
  19. Mitani, H., Koshiishi, I., Sumita, T. and Imanari, T. (2001). Prevention of the photodamage in the hairless mouse dorsal skin by kojic acid as an iron chelator. Eur. J. Pharmacol. 411, 169- 174. https://doi.org/10.1016/S0014-2999(00)00873-6
  20. Mitchell, R.E. (1967). Chronic solar dermatosis: a light and electron microscopic study of the dermis. J. Invest. Dermatol. 43, 203-230. https://doi.org/10.1038/jid.1964.39
  21. Montagna, W., Kirchner, S. and Carlisle, K. (1989). Histology of sun-damaged skin. J. Am. Acad. Dermatol. 21,907-918 https://doi.org/10.1016/S0190-9622(89)70276-0
  22. Oikamin, A and Kallionen, M. (1989). A biochemical andimmunohistochemical study of collagen in sun-exposed and protected skin. Photodermatol. 6, 24-31
  23. Olsen, E.A, Katz, H.I., Levine, N., Shupack, J., Billys, M.M., Prawer, S., Gold, J., Stiller, M., Lufrano, L. and Thome, E.G. (1992). Tretinoin emollient cream: a new therapy for photodamaged skin. J. Am. Acad. Dermatol. 26,215-224 https://doi.org/10.1016/0190-9622(92)70030-J
  24. Smith, J.G. Jr., Davidson, E.A., Sams, W.M. Jr. and Clark, R.D. (1962). Alterations in human dermal connective tissue with age and chronic sun damage. J. Invest. Dermatol. 39,347-350 https://doi.org/10.1038/jid.1962.122
  25. Taylor, C.R, Stem, R.S., Leyden, J.J. and Gilchrest, B.A. (1990). Photoaging/photodamage and photoprotection. J. Am. Acad. Dermatol. 22, 1-15 https://doi.org/10.1016/0190-9622(90)70001-X
  26. Tiedemann, K., Malmstrom, A. and Westergren-Thorsson, G. (1997). Cytokine regulation of proteoglycan production in fibroblasts: separate and synergistic effects. Matrix. Biol. 15, 469-478 https://doi.org/10.1016/S0945-053X(97)90020-2
  27. Warren, R, Gartstein, V., Kligman, A.M., Montagna, W., Allendorf, R.A.and Ridder, G.M. (1991). Age, sunlight, and facial skin: a histologic and quantitative study. J. Am. Acad. Dermatol. 25,751-760 https://doi.org/10.1016/S0190-9622(08)80964-4
  28. Weinstein, G.D., Nigra, T.P., Pochi, P.E., Savin, R.C., Allan, A, Benik, K., Jeffes, E., Lufrano, L. and Thome, E.G. (1991). Topical tretinoin for treatment of photodamaged skin: a multicenter study. Arch. Dermatol. 127, 659-665. https://doi.org/10.1001/archderm.127.5.659