Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes

  • Park, C.S. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Kim, M.Y. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Yi, Y.J. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Chang, Y.J. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Lee, S.H. (College of Visual Image & Health, Kongju National University) ;
  • Lee, J.J. (Korea Thumb Vet. Co., LTD.) ;
  • Kim, M.C. (College of Veterinary Medicine, Chungnam National University) ;
  • Jin, D.I. (Division of Animal Science & Resources, Research Center for Transgenic Cloned Pigs Chungnam National University)
  • Received : 2003.12.02
  • Accepted : 2004.05.13
  • Published : 2004.10.01
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Abstract

The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at $4^{\circ}C$ with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and $10{\times}10^6$ sperm/ml than in 0.2 and $1{\times}10^6$ sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in $10{\times}10^6$ sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in $1{\times}10^6$sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at $4^{\circ}C$ could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend $1{\times}10^6$sperm/ml concentration for in vitro fertilization of pig oocytes.

Keywords

References

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