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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Molecular Characterization of a thiJ-like Gene in Chinese Cabbage

  • Oh, Kyung-Jin (Division of Life Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Park, Yong-Soon (Division of Life Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Kyung-Ah (Division of Life Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Chung, Yong-Je (Division of Life Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Cho, Tae-Ju (Division of Life Sciences, College of Natural Sciences, Chungbuk National University)
  • Published : 2004.05.31
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Abstract

A cDNA clone for a salicylic acid-induced gene in Chinese cabbage (Brassica rapa subsp. pekinensis) was isolated and characterized. The cabbage gene encoding a protein of 392 amino acids contained a tandem array of two thiJ-like sequences. ThiJ is a thiamin biosynthesis enzyme that catalyzes the phosphorylation of hydroxymethylpyrimidine (HMP) to HMP monophosphate. Although the cabbage gene shows a similarity to bacterial thiJ genes, it also shares a similarity with the human DJ-1, a multifunctional protein that is involved in transcription regulation, male fertility, and parkinsonism. The cabbage thiJ-like gene is strongly induced by salicylic acid and a nonhost pathogen, Pseudomonas syringae pv. tomato, which elicits a hypersensitive response in Chinese cabbage. Treatment of the cabbage leaves with BTH, methyl jasmonate, or ethephon showed that the cabbage thiJ-like gene expression is also strongly induced by BTH, but not by methyl jasmonate or ethylene. This indicates that the cabbage gene is activated via a salicylic acid-dependent signaling pathway. Examination of the tissue-specific expression revealed that the induction of the cabbage gene expression by BTH occurs in the leaf, stem, and floral tissues but not in the root.

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References

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