Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

A histochemical study of argentaffin endocrine cells in the gastrointestinal tract of ovariectomized rats

  • Ku, Sae-kwang (Pharmacology & Toxicology Lab., Central Research Laboratories, Dong-Wha Pharm. Ind. Co.) ;
  • Lee, Hyeung-sik (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Lee, Jae-hyun (Department of Histology, College of Veterinary Medicine, Kyungpook National University)
  • Accepted : 2004.03.03
  • Published : 2004.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The regional distributions and frequencies of argentaffin endocrine cells in gastrointestinal (GI) tract of osteoporotic Sprague-Dawley rat induced by ovariectomy were studied by Masson-Hamperl silver stain. The experimental animals were divided into two groups, one is non-ovariectomized group (Sham) and the other is ovariectomized group (OVX). Samples were collected from each part of GI tract (fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) at 10th week after ovariectomy or sham operation. Argentaffin cells were detected throughout the entire GI tract with various frequencies regardless of ovariectomy except for the rectum of OVX in which no cells were detected. Most of these argentaffin cells in the mucosa of GI tract were generally spherical or spindle in shape (open type cell) while cells showing round in shape (close type cell) were rarely found in gland regions. Significant decrease of argentaffin cells was detected in OVX compared to that of Sham except for the fundus and jejunum. However, in the fundus and jejunum, argentaffin cells in OVX showed similar frequency compared to that of Sham. In conclusion, the endocrine cells are the anatomical units responsible for the production of gut hormones that regulate gut motility and digestion including absorption, and a change in their density would reflect the change in the capacity of producing these hormones and regulating gut motility and digestion. Ovariectomy induced severe quantitative changes of GI argentaffin endocrine cell density, and the abnormality in density of GI endocrine cells may contribute to the development of gastrointestinal symptoms in osteoporosis such as impairments of calcium and some lipids, frequently encountered in patients with postmenopausal osteoporosis.

Keywords

References

  1. Bell, F. R. The relevance of the new knowledge of gastrointestinal hormones to veterinary science. Vet. Sci. Commun. 1979, 2, 305-314
  2. Bishop, A. E. and Polak, J. M. Gastrointestinal endocrine tumours. Pathology. Baillieres Clin. Gastroenterol. 1996, 10, 555-569
  3. El-Salhy, M. The nature and implication of intestinal endocrine cell changes in celiac disease. Histol. Histopathol. 1998, 13, 1069-1075
  4. El-Salhy, M. and Sitohy, B. Abnormal gastrointestinal endocrine cells in patients with diabetes type I: relationship to gastric emptying and myoelectricalactivity. Scand. J. Gastroenterol. 2001, 36, 1162-1169
  5. Kalu, D. N. The ovariectomized rat model of postmenopausal bone loss. Bone Miner. Res. 1991, 15, 175-191
  6. Kalu, D. N. and Chen, C. Ovariectomized murine model of postmenopausal calcium malabsorption. J. Bone Miner. Res. 1999, 14, 593-601
  7. Lee, H. S. and Ku, S. K. Appearance of gastrointestinal endocrine cells in the fetus of the Korean native goat. J. Basic Sci. 1997, 1, 27-35
  8. Loest, H. B., Noh, S. K. and Koo, S. I. Green tea extract inhibits the lymphatic absorption of cholesterol and alpha-tocopherol in ovariectomized rats. J. Nutr.2002, 132, 1282-1288
  9. Lucini, C., De Girolamo, P., Coppola, L., Paino, G. and Castaldo, L. Postnatal development of intestinal endocrine cell populations in the water buffalo. J. Anat.1999, 195(Pt 3), 439-446
  10. Lundqvist, M., Arnberg, H., Candell, J., Malmgren, M., Wilander, E., Grimelius, L. and Oberg, K. Silver stains for identification of neuroendocrine cells. A studyof the chemical background. Histochem. J. 1990, 22, 615-623
  11. Mangoud, A. M., Badr, M. A. and Morsy, T. A. An acute case of infantile visceral leishmaniasis histopathological and histochemical studies. J. EgyptSoc. Parasitol. 1992, 22, 617-621
  12. Mathan, M. M., Chandy, G. and Mathan, V. I. Ultrastructural changes in the upper small intestinal mucosa in patients with cholera. Gastroenterology1995, 109, 422-430
  13. Mitamura, R., Hara, H., Aoyama, Y. and Chiji, H. Supplemental feeding of difructose anhydride III restores calcium absorption impaired by ovariectomy in rats. J. Nutr. 2002, 132, 3387-3393
  14. O'Toole, K., Fenoglio-Preiser, C. and Pushparaj, N. Endocrine changes associated with the human aging process: III. Effect of age on the number of calcitonin immunoreactive cells in the thyroid gland. Hum. Pathol. 1985, 16, 991-1000
  15. Portela-Gomes, G. M. and Grimelius, L. Identification and characterization of enterochromaffin cells with different staining techniques. Acta Histochem. 1986,79, 161-174
  16. Portela-Gomes, G. M., Grimelius, L., Johansson, H., Bergstrom, R. and Lundqvist, G. The effects of gastroenteroanastomosis, antral exclusion and antralresection on the enterochromaffin cells in the rat gastrointestinal tract. Acta Chir. Scand. 1987, 153, 669-675
  17. Riggs, B. L. Endocrine causes of age-related bone loss and osteoporosis. Novartis Found Symp. 2002, 242, 247-259
  18. Singh, I. A modification of the Masson-Hamperl method for staining of argentaffin cells. Anat. Anz. 1964, 115, 81-82
  19. Solcia, E., Capella, C., Vassallo, G. and Buffa, R. Endocrine cells of the gastric mucosa. Int. Rev. Cytol. 1975, 42, 223-286
  20. Solcia, E., Fiocca, R., Rindi, G., Villani, L., Luinetti, O., Burrell, M., Bosi, F. and Silini, E. Endocrine tumors of the small and large intestine. Pathol. Res.Pract. 1995, 191, 366-372
  21. Sternberger, L. A. The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Sternberger, L. A. (ed), Immunocytochemistry, pp. 104-169, John Wiley & Sons, New York, 1979
  22. Tasca, C., Secareanu-Chelaru, E. and Budu, S. The enteroendocrine cells during the normal development of the rat and in some human digestive diseases.Endocrinologie 1978, 16, 209-212
  23. Thomas, T. Leptin: a potential mediator for protective effects of fat mass on bone tissue. Joint Bone Spine 2003, 70, 18-21
  24. Yamaguchi, K., Yada, M., Tsuji, T., Kuramoto, M. and Uemura, D. Suppressive effect of norzoanthamine hydrochloride on experimental osteoporosis inovariectomized mice. Biol. Pharm. Bull. 1999, 22, 920-924