대한수의학회지 (Korean Journal of Veterinary Research)

  • 제44권3호
  • /
  • Pages.407-413
  • /
  • 2004
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

개의 임상재료로부터 분리된 포도상구균의 균종분포 및 항균제 감수성

Species distribution and antimicrobial susceptibility of staphylococci isolated from canine clinical specimens

  • Park, Cheong-Kyu (College of Veterinary Medicine, Kyungpook National University) ;
  • Choi, Seong-Mi (College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Young-Ju (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University) ;
  • Yeo, Sang-Geon (College of Veterinary Medicine, Kyungpook National University)
  • 심사 : 2004.08.16
  • 발행 : 2004.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Specimens collected from various pyogenic lesions of dogs were culturally examined for staphylococci and all staphylococcal isolates obtained from the specimens were also tested for susceptibility to 14 antimicrobial agents. A total of 123 isolates of staphylococci were identified. Of these, 120 were Staphylococcus intermedius and 3 were S aureus. All isolates were susceptible to oxacillin, cefazolin, cephalothin and amikacin, whereas more than 85% of the isolates were resistant to ampicillin, penicillin G and tetracycline. S intermedius isolates could be divided into 8 different biotypes by biotyping with the most common type accounting for 66.7% of the isolates. One hundred and seventeen(97.5%) isolates could be also divided into 26 different antibiogram patterns. The predominant antibiogram type accounted for 34.2% of the isolates. Antibiogram typing was found to be effective in distinguishing epidemiologically related isolates of S intermedius.

키워드

참고문헌

  1. Aarestrup, F. M., Wegener, H. C. and Rosdahl, V. T. Evaluation of phenotypic and genotypic methods for epidemiological typing of Staphylococcus aureus isolates from bovine mastitis in Denmark. Vet. Microbiol. 1995, 45, 139-150
  2. Berg, J. N., Wendell, D. E., Vogelweid, C. and Fales, W. H. Identification of the major coagulase-positive Staphylococcus sp of dogs as Staphylococcus intermedius. Am. J. Vet. Res. 1984, 45, 1307-1309
  3. Biberstein, E. L., Jang, S. S. and Hirsh, D. C. Species distribution of coagulase-positive staphylococci in animals. J. Clin. Microbiol. 1984, 19, 610-615
  4. Case, L. C., Ling, G. V., Biberstein, E. L., Ruby, A. L. and Jang, S. S. Staphylococci in canine urolithiasis: Species identification, using a commercially available tray micromethod. Am. J. Vet. Res. 1985, 46, 238-241
  5. Chaffer, M., Leitner, G., Winkler, M. and Saran, A. Coagulase-negative Staphylococcus intermedius isolated from milk from dairy cows in Israel. Vet. Rec. 1998, 143, 592-593
  6. Cole, L. K., Kwochka, K. W., Kowalski, J. J. and Hillier, A. Microbial flora and antimicrobial susceptibility patterns of isolated pathogens from the horizontal ear canal and middle ear in dogs with otitis media. J. Am. Vet. Med. Assoc. 1998, 212, 534-538
  7. Cox, H. U., Newman, S. S., Roy, A. F. and Hoskins, J. D. Species of Staphylococcus isolated from animal infections. Cornell Vet. 1984, 74, 124-135
  8. Cox, H. U., Schmeer, N. and Newman, S. S. Protein A in Staphylococcus intermedius isolates from dogs and cats. Am. J. Vet. Res. 1986, 47, 1881-1884
  9. Devriese, L. A. and De Pelsmaecker, K. The anal region as a main carrier site of Staphylococcus intermedius and Streptococcus canis in dogs. Vet. Rec. 1987, 121, 302-303
  10. Devriese, L. A. and Van de Kercknove, A. A. comparison of methods used for testing staphylokinase (fibrinolysin) production in Staphylococcus strains. Antonie van Leeuwenhoek. 1980, 46, 457-465
  11. Hajek, V. Staphylococcus intermedius, a new species isolated from animals. Int. J. Syst. Bact. 1976, 26, 401-408
  12. Hajek, V. and Mar alek, E. The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification. Zbl. Bakt. Hyg. Abt. 1 Orig. 1971, A217, 176-182
  13. Harvey, R. G. Tylosin in the treatment of canine superficial pyoderma. Vet. Rec. 1996, 139, 185-187
  14. Ihrke, P. J. An overview of bacterial skin disease in the dog. Br. Vet. J. 1987, 143, 112-118
  15. Kawano, J., Shimizu, A. and Kimura, S. Isolation of phages for typing of Staphylococcus intermedius isolated from horses. Jpn. J. Vet. Sci. 1981, 43, 933-936
  16. Kawano, J., Shimizu, A., Kimura, S. and Blouse, L. Experimental bacteriophage set for typing Staphylococcus intermedius. Zbl. Bakt. Hyg. I. Abt. Orig. 1982, A253, 321-330
  17. Lachica, R. V. F., Genigeorgis. C. and Hoeprich. P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 1971, 21, 585-589
  18. Ling, G. V., Franti, C. E., Johson, D. L. and Ruby, A. L. Urolithiasis in dogs IV: Survey of interrelations among breed, mineral composition, and anatomic location of calculi, and presence of urinary tract infection. Am. J. Vet. Res. 1998, 59, 650-660
  19. Mahoudeau, I., Delabranche, X., Prevost, G., Monteil, H. and Piemont, Y. Frequency of isolation of Staphylococcus intermedius from humans. J. Clin.Microbiol. 1997, 35, 2153-2154
  20. Mcewan, N. A. Adherence by Staphylococcus intermedius to canine keratinocytes in atopic dermatitis. Res. Vet. Sci. 2000, 68, 279-283
  21. Medleau, L., Long, R. E., Brown, J. and Miller, H. Frequency and antimicrobial susceptibility of Staphylococcus species isolated from canine pyodermas. Am. J. Vet. Res. 1986, 47, 229-231
  22. Molenda, J., Werner-Dabrowska, B., Leska, A. and Dabrowski, M. Sensitivity of canine Staphylococcus intermedius strains to the phages typing Staphylococcusaureus isolated from man. Pol. Arch. Weter. 1990, 30, 13-20
  23. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated fromanimals; Approved standard-fifth ed., M 31-A, 2000
  24. Overturf, G. D., Talan, D. A., Singer, K., Anderson, N., Miller, J. I., Greene, R. T. and Froman, S. Phage typing of Staphylococcus intermedius. J. Clin. Microbiol.1991, 29, 373-375
  25. Phillips, W. E. and Williams, B. J. Antimicrobial susceptibility patterns of canine Staphylococcus intermedius isolates from veterinary clinical specimens. Am. J. Vet. Res. 1984, 45, 2376-2379
  26. Raus, J. and Love, D. N. Characterization of coagulase-positive Staphylococcus intermedius and Staphylococcus aureus isolated from veterinary clinical specimens. J. Clin. Microbiol. 1983, 18, 789-792
  27. Roberson, J. R., Fox, L. K., Hancock, D. D., Gay, J. M. and Besser, T. E. Evaluation of methods for differentiation of coagulase-positive staphylococci. J.Clin. Microbiol. 1992, 30, 3217-3219
  28. Smith, R. F. and Willett, N. P. Rapid plate method for screening hyaluronidase and chondroitin sulfataseproducing microorganisms. Appl. Microbiol. 1968, 16,1434-1436
  29. Talan, D. A., Staatz, D., Staatz, A., Goldstein, E. J. C., Singer, K. and Overturf, G. D. Staphylococcus intermedius in canine gingiva and canine-inflictedhuman wound infections : Laboratory characterization of a newly recognized zoonotic pathogen. J. Clin. Microbiol. 1989, 27, 78-81
  30. Talan, D. A., Staatz, D., Staatz, A. and Overturf, G. D. Frequency of Staphylococcus intermedius as human nasopharyngeal Flora. J. Clin. Microbiol. 1989, 27, 2393
  31. Tanner, M. A., Everett, C. L. and Youvan, D. C. Molecular phylogenetic evidence for noninvasive zoonotic transmission of Staphylococcus intermedius from a canine pet to a human. J. Clin. Microbiol. 2000, 38, 1628-1631
  32. Tenover, F. C., Arbeit, R., Archer, G., Biddle, J., Byrne, S., Goering, R., Hancock, G., Hebert, G. A., Hill, B., Hollis, R., Jarvis, W. R., Kreiswirth, B.,Eisner, W., Maslow, J., McDougal, L. K., Miller, J. M., Mulligan, N. and Pfaller, M. A. Comparison of traditional and molecular methods of typing isolates ofStaphylococcus aureus. J. Cli. Microbiol. 1994, 32, 407-415