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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Association between the Angiotensin-converting Enzyme Gene Insertion/Deletion Polymorphism and Essential Hypertension in Young Pakistani Patients

  • Ismail, Muhammad (Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories) ;
  • Akhtar, Naveed (Department of Cardiology, KRL General Hospital) ;
  • Nasir, Muhammad (Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories) ;
  • Firasat, Sadaf (Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories) ;
  • Ayub, Qasim (Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories) ;
  • Khaliq, Shagufta (Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories)
  • Published : 2004.09.30
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Abstract

Several studies have demonstrated the importance of angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) polymorphisms in the pathogenesis of hypertension. This study sought to determine the association between the ACE I/D polymorphism and essential hypertension in young Pakistanis. The frequency of the ACE I/D polymorphism was established by a comparative cross-sectional survey of Pakistani patients suffering from essential hypertension and ethnically matched normotensive controls. Samples were collected from tertiary care hospitals in northern Pakistan. Hypertensive individuals were defined as those with a systolic blood pressure > 140 mmHg and/or diastolic blood pressure > 90 mmHg on three separate occasions, or those currently receiving one, or more, anti-hypertensive agents. DNA samples obtained from hypertensive (n=211) and normotensive (n=108) individuals were typed by PCR. The frequency of the ACE I/I genotype was significantly higher in hypertensive patients, aged 20-40 years, than in normotensive controls of the same age group ($\chi^2$ = 4.0, P = 0.041). Whereas no overall significant differences were observed between the I/I, I/D and D/D ACE genotypes (One way ANOVA, F=0.672; P=0.413). The association between the ACE I/I genotype and essential hypertension in individuals aged $\leq$ 40 years suggests that ACE has a role in early onset essential hypertension in Pakistan.

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References

  1. Ayub, Q., Mansoor, A., Ismail, M., Khaliq, S., Mohyuddin, A., Hameed, A., Mazhar, K., Rehman, S., Papaioannou, A. P., Cavalli-Sforza, L. L. and Mehdi, S. Q. (2003) Reconstruction of human evolutionary tree using polymorphic autosomal microsatellites. Am. J. Physical. Anthropol. 121, 259-268.
  2. Barley, J., Blackwood, A., Miller, M., Markandu, N. D., Carter, N. D., Jeffery, S., Cappuccio, F. P., MacGregor, G. A. and Sagnella, G. A. (1996) Angiotensin converting enzyme gene I/D polymorphism, blood pressure and the rennin-angiotensin system in Caucasian and Afro-Caribbean peoples. J. Hum. Hypertens. 10, 31-35.
  3. Batzer, M. A., Arcot, S. S., Phinney, J. W., Alergria-Hartman, M., Kass, D. H., Milligan, S. M., Kimpton, C., Gill, P., Hochmeister, M., Ioannou, P. A., Herrera, R. J., Boudreau, D. A., Scheer, W. D., Keats, B. J. B., Deininger, P. L. and Stoneking, M. (1996) Genetic variation of recent Alu insertions in human populations. J. Mol. Evol. 42, 22-29. https://doi.org/10.1007/BF00163207
  4. Chiang, F. T., Chern, T. H., Lai, Z. P., Tseng, C. D., Hsu, K. L., Lo, H. M. and Tseng, Y. Z. (1996) Age- and genderdependent association of the angiotensin-converting enzyme gene with essential hypertension in a Chinese population. J. Hum. Hypertens. 10, 823-826.
  5. Chiang, F. T., Lai, Z. P., Chern, T. H., Tseng, C. D., Hsu, K. L., Lo, H. M. and Tseng, Y. Z. (1997) Lack of association of the angiotensin converting enzyme gene polymorphism with essential hypertension in a Chinese population. Am. J. Hypertens. 10, 197-201. https://doi.org/10.1016/S0895-7061(96)00345-7
  6. Duru, K., Farrow, S., Wang, J. M., Lockette, W. and Kurtz, T. (1994) Frequency of a deletion polymorphism in the gene for angiotensin converting enzyme is increased in African-Americans with hypertension. Am. J. Hypertens. 7, 759-762.
  7. Erdos, E. and Skidgel, R. A. (1987) The angiotensin I-converting enzyme. Lab. Invest. 56, 345-348.
  8. Higashimori, K., Zhao, Y., Higaki, J., Kamitani, A., Katsuya, T., Nakura, J., Miki, T., Mikami, H. and Ogihara, T. (1993) Association analysis of a polymorphism of the angiotensin converting enzyme gene with essential hypertension in the Japanese population. Biochem. Biophys. Res. Commun. 191, 399-404. https://doi.org/10.1006/bbrc.1993.1231
  9. Jafar, T. H., Levey, A. S., Jafary, F. H., White, F., Gul, A., Rahbar, M. H., Khan, A. Q., Hattersley, A., Schmid, C. H. and Chaturvedi, N. (2003) Ethnic subgroup differences in hypertension in Pakistan. J. Hypertens. 21, 905-912. https://doi.org/10.1097/00004872-200305000-00014
  10. Jeng, J. R., Harn, H. J., Jeng, C. Y., Yueh, K. C. and Shieh, S. M. (1997) Angiotensin I converting enzyme gene polymorphism in Chinese patients with hypertension. Am. J. Hypertens. 10, 558-561. https://doi.org/10.1016/S0895-7061(97)00036-8
  11. Ludwig, E., Corneli, P. S., Anderson, J. L., Marshall, H. W., Lalouel, J. M. and Ward, R. H. (1995) Angiotensin-converting enzyme gene polymorphism is associated with myocardial infarction but not with development of coronary stenosis. Circulation 91, 2120-2124. https://doi.org/10.1161/01.CIR.91.8.2120
  12. Nistar, S. (2002) Prevention of coronary heart disease in south Asia. Lancet 360, 1015-1018. https://doi.org/10.1016/S0140-6736(02)11088-9
  13. Niu, T., Chen, X. and Xu, X. (2002) Angiotensin converting enzyme gene insertion/deletion polymorphism and cardiovascular disease, therapeutic implications. Drugs 62, 977-993. https://doi.org/10.2165/00003495-200262070-00001
  14. Pakistan Medical Research Council. (1998) National Health Survey of Pakistan, 1990-94, Health Profile of the People of Pakistan, Network Publication Service, Islamabad, Pakistan
  15. Raynolds, M. V., Bristow, M. R., Bush, E. W., Abraham, W. T., Lowes, B. D., Zisman, L. S., Taft, C. S. and Perryman, M. B. (1993) Angiotensin-converting enzyme DD genotype in patients with ischaemic or idiopathic dilated cardiomyopathy. Lancet 342, 1073-1075. https://doi.org/10.1016/0140-6736(93)92061-W
  16. Rigat, B., Hubert, C., Alhenc-Gelas, F., Cambien, F., Corvol, P. and Soubrier, F. (1990) An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J. Clin. Invest. 86, 1343-1346. https://doi.org/10.1172/JCI114844
  17. Sagnella, G. A., Rothwell, M. J., Onipinla, A. K., Wicks, P. D., Cook, D. G. and Cappuccio, F. P. (1999) A population study of ethnic variations in the angiotensin-converting enzyme I/D polymorphism, relationships with gender, hypertension and impaired glucose metabolism. J. Hypertens. 17, 657-664. https://doi.org/10.1097/00004872-199917050-00009
  18. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York, USA
  19. Schunkert, H., Hense, H. W., Holmer, S. R., Stender, M., Perz, S., Keil, U., Lorell, B. H. and Riegger, G. A. (1994) Association between a deletion polymorphism of the angiotensinconverting-enzyme gene and left ventricular hypertrophy. N. Engl. J. Med. 330, 1634-1638. https://doi.org/10.1056/NEJM199406093302302
  20. Vassilikioti, S., Doumas, M., Douma, S., Petidis, K., Karagiannis, A., Balaska, K., Vyzantiadis, A. and Zamboulis, C. (1996) Angiotensin converting enzyme gene polymorphism is not related to essential hypertension in a Greek population. Am. J. Hypertens. 9, 700-702. https://doi.org/10.1016/0895-7061(95)00449-1

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