Comparison of tissue tropism of Newcastle disease vaccine viruses by Immunohistochemistry techniques

면역조직화학기법을 이용한 뉴캣슬병 백신바이러스의 조직친화성 비교

  • Kim, Min-Jeong (National Veterinary Research and Quarantine Service) ;
  • Kwon, Yong-Kuk (National Veterinary Research and Quarantine Service) ;
  • Seong, Hwan-Woo (National Veterinary Research and Quarantine Service) ;
  • Kang, Shien-Young (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Mo, In-Pil (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
  • 김민정 (국립수의과학검역원) ;
  • 권용국 (국립수의과학검역원) ;
  • 성환우 (국립수의과학검역원) ;
  • 강신영 (충북대학교 수의과대학 동물의학연구소) ;
  • 모인필 (충북대학교 수의과대학 동물의학연구소)
  • Accepted : 2004.11.27
  • Published : 2004.12.30


Mean death time of inoculated embryonated egg is one of the methods to determine the virulence of the Newcastle disease viruses (NDV). Evaluation of tissue tropism of NDV in the host has been proposed as an another way to determine the pathogenicity of NDV based on the principal site of viral replication. To evaluate the tissue tropism among NDV, an immunohistochemistry(IHC) technique using monoclonal antibody was applied in one-day-old SPF chickens inoculated with different ND vaccine strains such as Ulster 2C, VG/GA and B1 viruses by eye drop instillation. The tissues used for this comparison were trachea, intestine, Harderian gland and cecal tonsil, which were collected at 0.5, 1, 2, 3, 5, 8, 10, 14 days post inoculation. Among test groups, chickens inoculated with B1 viurs, which is known to replicate in the respiratory system, have IHC positive staining mainly in the trachea and those inoculated with Ulster 2C have IHC positive staining mainly in the intestine. However, chickens inoculated with VG/GA strain have IHC positive staining in both the trachea and intestine. Therefore, a differences in tissue tropism among ND vaccine strains has been proved by the IHC technique. Based on this results, the IHC staining technique could be used to classify the NDV or to study the pathogenesis of NDV in chickens.



Supported by : 충북대학교


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