Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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The survey of Trichinella spiralis infection in finishing pigs using the pepsin-digestion method and ELISA in Korea

조직인공소화법과 ELISA를 이용한 국내 출하돈의 선모충(Trichinella spiralis) 감염실태 조사

  • Seo, Hunsu (Pfizer Animal Health Korea Ltd.) ;
  • Woo, Gye-Hyeong (Graduate School of Agricultural and Life Sciences, The University of Tokyo) ;
  • Youn, Hee-Jeong (College of Veterinary Medicine, Seoul National University)
  • 서흔수 (한국화이자동물약품(주)) ;
  • 우계형 (동경대학교 농업생명과학부) ;
  • 윤희정 (서울대학교 수의과대학)
  • Accepted : 2004.04.22
  • Published : 2004.06.30
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Abstract

Trichinella spiralis is one of the important zoonotic parasites with a wide variety of vertebrates hosts in nature. The purpose of this study were to analyze ESP(Excretory-Secretory Protein) antigen, to evaluate ELISA for the serological diagnosis of Trichinosis, and to survey T. spiralis infection in finishing pigs using the pepsin digestion method and ELISA in Korea. In the analysis of ESP antigen by SDS-PAGE and Western blot, 4 major bands (70, 55, 52.6, and 49 kDa) were revealed from the ESP antigen. Predilection sites of T. spiralis were the diaphragm, the tongue, masseter muscles, intercostal muscle, and hindlimb in orders in the experimentally infected rats. Sera from 581 swine were tested by ELISA with ESP antigen. The 54 (9.3%) sera were suspected as positive reactors, however, these 54 sera were determined as false positives by the use of Western blotting. This study demonstrated that the ELISA was not suitable for the examination of T. spiralis in pork. The diaphragm muscle samples of 251 finishing pigs were tested by the method of pepsin-digestion for the presence of Trichinella larvae, however, T. spiralis was not detected from the samples. We could not find out T. spiralis infection in pig in Korea pork.

Keywords

References

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