Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Effect of scan-bio laser therapy on arthropathy in rabbits

토끼의 관절병증에 미치는 SCAN-BIO 레이저의 치료효과

  • Cho, Hyung-Jin (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • Kim, Young-Su (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • Oh, Dong-Min (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • Sim, Kyung-Mi (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • Kang, Seong-Soo (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University) ;
  • Lim, Sung-Chul (Department of Pathology and Research Center for Resistant Cells, Chosun University, Medical School) ;
  • Cho, Yong-Seong (College of Veterinary Medicine, Konkuk University) ;
  • Lee, Soo-Han (College of Veterinary Medicine, Konkuk University) ;
  • Choi, Seok-Hwa (College of Veterinary Medicine, Chungbuk National University) ;
  • Bae, Chun-Sik (College of Veterinary Medicine, Biotechnology Research Institute, Chonnam National University)
  • 조형진 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 김영수 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 오동민 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 심경미 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 강성수 (전남대학교 수의과대학 및 생물공학연구소) ;
  • 임성철 (조선대학교 의과대학 및 내성세포연구센터) ;
  • 조용성 (건국대학교 수의과대학) ;
  • 이수한 (건국대학교 수의과대학) ;
  • 최석화 (충북대학교 수의과대학) ;
  • 배춘식 (전남대학교 수의과대학 및 생물공학연구소)
  • Accepted : 2004.06.29
  • Published : 2004.09.30
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Abstract

For the induction of arthropathy, 4% hydrogen peroxide ($H_2O_2$) was injected for 4 weeks into the intra-articular space of the 25 New Zealand white rabbits to damage articular cartilage. The verification of arthropathy induction and the effect of scan-bio laser treatment were determined by measuring superoxide dismutase (SOD) activity, by observing gross and histopathologic findings. The SOD activity increased by about 40% in arthropathy group, as compared to controls. Although SOD activity in arthropathy group was not significantly different from the 2-week group, it was significantly different from the 4-week control and treatment groups. There was also a significant difference between the 4-week control and treatment groups. Grossly, erosions formed on the articular cartilage surface, and the lateral femoral condyle was damaged in arthropathy group. In comparison, there was slight, but not significant, progression of the lesion in the 2-week control group, and no difference between the 2-week treatment and control groups. Conversely, severe erosions damaged the articular cartilage in the 4-week control group. Cartilage proliferation was seen in gross observations in the 4-week treatment group, suggesting a treatment effect. Histopathologically, there was slight articular surface damage and apoptosis in arthropathy group, and serious cartilage damage, despite slight chondrocyte proliferation, in the 4-week control group. By contrast, the 4-week treatment group showed chondrocyte replacement, with close to normal articular cartilage on the articular surface. There was significant cartilage proliferation with regeneration of the articular cartilage on the articular surface in the group treated with low-level laser, as compared to control group, when arthropathy was induced by $H_2O_2$ injections. Therefore, low-level laser was effective in the treatment of chemically induced arthropathy.

Keywords

References

  1. 배영훈, 안태훈, 임성철, 박석천, 이재창, 강남현, 배춘식. 토끼의 관절병증에 미치는 알긴산올리고당의 치료효과. 대한수의학회지. 2002, 42, 153-162
  2. 신동민, 문영래, 이병래. 골성 관절염 환자에서 적혈구 및 활액의 superoxide dismutase, catalase 및 glutathione peroxidase 활성. 대한정형외과학회지. 1994, 29, 44-49
  3. 이병호, 하상호, 유재원, 이상홍, 표영배, 김광, 이병래. 과산화수소와 3-aminotriazol이 가토의 슬관절에 미치는 영향. 대한정형외과학회지. 2000, 35, 589-594
  4. 이진원, 김창환, 박종환, 최은실, 홍정주, 박재섭, 박재학. 골관절증이 유발된 토끼에서 Sodium Hyaluronate의 효과. 응용약물학회지. 1999, 7, 347-353
  5. 이태현, 손덕희, 김봉일, 조성경, 이상화. 저출력 레이저 조사에 의한 창상의 통증완화 및 치유조장. 대한통증학회지. 1994, 7, 74-77
  6. Abatangelo, G., Botti, P., Gei, G., Samson, J. C., Cortivo, R., De Galateo, A. and Martelli, M. Intraarticular sodium hyaluronate injection in the Pond-Nuki experimental model of osteoarthritis in dogs. I. Biochemical results. Clin Orthop. 1989, 241, 278-285
  7. Amano, A., Miyagi, K., Azuma, T., Ishihara, Y., Katsube, S., Aoyama, I. and Saito, I. Histological studies on the rheumatoid synovial membrane irradiatedwith a low energy laser. Lasers Surg Med. 1994, 15, 290-294
  8. Basford, J. R. Laser therapy: scientific basis and clinical role. Orthopedics. 1993, 16, 541-547
  9. Beckerman, H., de Bie, R. A., Bouter, L. M., De Cuyper, H. J. and Oostendorp, R. A. The efficacy of laser therapy for musculoskeletal and skin disorders:a criteria-based meta-analysis of randomized clinical trials. Phys. Ther. 1992, 72, 483-491
  10. Brosseau, L., Welch, V., Wells, G., Tugwell, P., de Bie, R., Gam, A., Harman, K., Shea, B. and Morin, M. Low level laser therapy for osteoarthritis andrheumatoid arthritis: a metaanalysis. J. Rheumatol. 2000, 27, 1961-1969
  11. Clemmons, D. R., Busby, W. H. Jr, Garmong, A., Schultz, D. R., Howell, D. S., Altman, R. D. and Karr, R. Inhibition of insulin-like growth factor bindingprotein 5 proteolysis in articular cartilage and joint fluid results in enhanced concentrations of insulin-like growth factor 1 and is associated with improved osteoarthritis. Arthritis Rheum. 2002, 46, 694-703
  12. Fulga, C. Antiinflammatory effect of laser therapy in rheumatoid arthritis. Rom. J. Intern. Med. 1998, 36, 273-279
  13. Hunziker, E. B. Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthritis Cartilage. 2002, 10, 432-463
  14. Impellizeri, J. A., Tetrick, M. A. and Muir, P. Effect of weight reduction on clinical signs of lameness in dogs with hip osteoarthritis. J. Am. Vet. Med. Assoc. 2000, 216, 1089-1091
  15. Kaiki, G., Tsuji, H., Yonezawa, T., Sekido, H., Takano, T., Yamashita, S., Hirano, N. and Sano, A. Osteoarthrosis induced by intra-articular hydrogenperoxide injection and running load. J. Orthop. Res. 1990, 8, 731-740
  16. McLaughlin, R. Management of chronic osteoarthritic pain. Vet. Clin. North Am. Small Anim. Pract. 2000, 30, 933-949
  17. Messner, K., Fahlgren, A., Ross, I. and Andersson, B. Simultaneous changes in bone mineral density and articular cartilage in a rabbit meniscectomy model ofknee osteoarthrosis. Osteoarthritis Cartilage. 2000, 8, 197-206
  18. Mitrovic, D. R., Garcia, F., Front, P. and Guillermet, V. Histochemical and cellular changes induced in the rabbit knee joint by an intraarticular implantation of a sheet of polyethylene. Lab. Invest. 1985, 53, 228-239
  19. Oegema, T. R. Jr, Carpenter, R. J., Hofmeister, F. and Thompson, R. C. Jr. The interaction of the zone of calcified cartilage and subchondral bone in steoarthritis. Microsc. Res. Tech. 1997, 37, 324-332
  20. Pelletier, J. P., Lajeunesse, D., Hilal, G., Jovanovic, D., Fernandes, J. C. and Martel-Pelletier, J. Carprofen reduces the structural changes and the abnormal subchondral bone metabolism of experimental osteoarthritis. Osteoarthritis Cartilage. 1999, 7, 327-328
  21. Ratkay, L. G., Chowdhary, R. K., Neyndorff, H. C., Tonzetich, J., Waterfield, J. D. and Levy, J. G. Photodynamic therapy; a comparison with otherimmunomodulatory treatments of adjuvant-enhanced arthritis in MRL-lpr mice. Clin. Exp. Immunol. 1994, 95, 373-377
  22. Soffa, A. J., Markel, M. D., Converse, L. J., Massa, K. L., Bogdanske, J. J. and Dillingham, M. F. Treatment of inflammatory arthritis by synovial ablation:a comparison of the holmium: YAG laser, electrocautery, and mechanical ablation in a rabbit model. Lasers Surg. Med. 1996, 19, 143-151
  23. Tanaka, H., Kitoh, Y., Katsuramaki, T., Tanaka, M., Kitabayashi, N., Fujimori, S., Umemoto, J. and Namba, K. Effects of SL-1010 (sodium hyaluronatewith high molecular weight) on experimental osteoarthritis induced by intra-articularly applied papain in guinea pigs. Nippon Yakurigaku Zasshi. 1992, 100,77-86
  24. Timofeyev, V. T., Poryadin, G. V. and Goloviznin, M. V. Laser irradiation as a potential pathogenetic method for immunocorrection in rheumatoid arthritis.Pathophysiology. 2001, 8, 35-40
  25. Ulugol, A., Unalan, H., Dokmeci, I. and Kokino, S. Comparison of the effects of tenoxicam and mid-laser irradiation on chronic adjuvant arthritis in rats. Clin.Exp. Rheumatol. 1997, 15, 83-86
  26. van den Berg, W. B. Lessons from animal models of osteoarthritis. Curr. Opin. Rheumatol. 2001, 13, 452-456
  27. van Valburg, A. A., van Roermund, P. M., Marijnissen, A. C., Wenting, M. J., Verbout, A. J., Lafeber, F. P. and Bijlsma, J. W. Joint distraction in treatment of osteoarthritis (II): effects on cartilage in a canine model. Osteoarthritis Cartilage. 2000, 8, 1-8