Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Release of Newcastle Disease Virus Vaccine from Chitosan Microspheres In vitro and In vivo

  • Park, I.K. (School of Agricultural Biotechnology, Seoul National University) ;
  • Jiang, H.L. (School of Agricultural Biotechnology, Seoul National University) ;
  • Yun, C.H. (School of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Y.J. (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, S.J. (College of Veterinary Medicine, Seoul National University) ;
  • Akaike, T. (Department of Biomolecular Engineering, Tokyo Institute of Technology) ;
  • Kim, S.I. (School of Agricultural Biotechnology, Seoul National University) ;
  • Cho, C.S. (School of Agricultural Biotechnology, Seoul National University)
  • Received : 2003.06.11
  • Accepted : 2003.11.11
  • Published : 2004.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Newcastle disease vaccine (NDV)-loaded chitosan microspheres (NDV-CM) were prepared. Stimulatory effects of these NDV-CM on antibody response compared to free NDV were examined in vitro and in vivo. In vitro stimulation of macrophages with virus vaccine resulted in higher number of cells compared to saline-treated control. Both NDV and NDV-CM induced secretion of interleukin-1 (IL-1) in dose dependent manner and the secretion of IL-1 by NDV-CM was delayed compared to free NDV. Irrespective of vaccine formulation, NDV subunit antigen was not effective in preventing mortality of the birds after challenge. However, CM loaded with NDV made of whole viron had antibody responses and protection similar to those shown by ND-K, a commercial inactivated oilemulsion vaccine.

Keywords

References

  1. Alonso, M. J., R. K. Gupta, C. Min, G. R. Siber and R. Langer. 1994. Biodegradable microspheres as controlled-release tetanus toxoid delivery systems. Vaccine. 12:299-306.
  2. Estrada, A., C. H. Yun, A. Van Kessel, B. Li, S. Hauta and B. Laarveld. 1997. Immunomodulatory activities of oat betaglucan in vitro and in vivo. Microbiol. Immunol. 41:991-8.
  3. Hilgers, L. A. Th., I. Nicolas, G. Lejeune, E. Dewil, and B. Boon. 1998. Effects of various adjuvants on secondary immune response in chickens. Vet. Immunol. Immunopathol. 66:159-171.
  4. Illum, I. 1998. Chitosan and its use as a pharmaceutical excipient. Pharm. Res. 15:1326-1331.
  5. MI, F. L., S. S. Shyu, C. T. Chen and J. Y. Schoung. 1999. Porous chitosan microsphere for controlling the antigen release of Newcastle disease vaccine: preparation of antigen-adsorbed microsphere and in vitro release. Biomaterials. 20:1603-1612.
  6. Morris, W., M. C., Steinhoff and P. K. Russell. 1994. Potential of polymer microencapsulation technology for vaccine innovation. Vaccine. 12:5-11.
  7. Nishimura, K., S. Nishimura, N. Nishi, I. Saiki, S. Tokura and I. Azuma. 1984. Immunological activity of chitin and its derivatives. Vaccine. 2:93-94.
  8. Nishimura, K., S. Nishimura and I Azuma. 1985. Adjuvant activity of chitin derivatives in mice and guinea pigs. Vaccine. 3:379-384. https://doi.org/10.1016/0264-410X(85)90127-6
  9. Rehmani, S. F. and P. B. Spradbrow. 1995. The influence of adjuvants on oral vaccination of chickens against Newcastle disease. Vet. Microbiol. 46:63-68.
  10. Russell, P. K. Monoclonal antibodies in research, diagnosis and epizootiology of Newcastle disease. In: (Ed. D. J. Alexander), Newcastle Disease, pp. 131-146. Kluwer. Academic. Publ. Boston. 1988.
  11. Thayer, S. G. and C. W. Beard. Serological procedures. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens. 4th ed., The American Association of Avian Pathologists, University of Pennsylvania, New Bolton Center, Kennett Square, PA 19348-1692. pp. 255-266. 1998
  12. Yusoff, K. and W. S. Tan. 2001. Newcastle disease virus: macromolecules and opportunities. Avian Pathol. 30:439-455.

Cited by

  1. Chitin and Chitosan Preparation from Marine Sources. Structure, Properties and Applications vol.13, pp.3, 2015, https://doi.org/10.3390/md13031133
  2. Immune-stimulating potential of cell envelope proteins from Vibrio cholerae associated to chitosan microparticles: An in vitro study vol.40, pp.6, 2004, https://doi.org/10.3109/10731199.2012.696063