Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Establishment of micronuclus assay as biological dosimetry in pig lymphocytes after gamma-irradiation

돼지 림프구의 미소핵 형성을 지표로 방사선 생물학적 선량측정법 확립

  • Kim, Se-ra (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-june (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jin-hee (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Chang-mo (Korea Institute of Radiological & Medical Science) ;
  • Kim, Tae-hwan (College of Veterinary Medicine, Kyungpook National University) ;
  • Jo, Sung-kee (Korea Atomic Energy Research Institute) ;
  • Kim, Jong-choon (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-ho (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2004.08.16
  • Published : 2004.09.30
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Abstract

The purpose of the present experiment was to investigate the micronuclei(MN) frequency in cytokinesis-blocked(CB) cells after various doses of gamma-rays in pig (Landrace, male, 3-month-old) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors. The peaks of binucleated lymphocyte formation(22%) were found at a concentration of 2% phytohaemagglutinin(PHA) and $4{\mu}g/ml$ Cytochalasin B(Cyt-B) in pig at 72 hours after incubation. Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was $y=0.0183D+0.0124D^2+0.0133$(y = number of MN/CB cells and D=irradiation dose in Gy). In conclusion, the results demonstrate that it appears feasible to use pig as target organisms in the micronucleus test to estimate the cytogenetic damage caused by ionizing radiations or, potentially, chemical compounds.

Keywords

References

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