Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Expression of galectin-3 in the spinal cords of Lewis rats andNOD mice with experimental autoimmune encephalomyelitis

자기면역성뇌척수염 척수조직에서 galection-3의 발현

  • Kim, Heechul (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University) ;
  • Joo, Hong-Gu (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University) ;
  • Moon, Changjong (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University) ;
  • Ahn, Meejung (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University) ;
  • Jee, Youngheun (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University) ;
  • Lim, Yoon-kyu (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University) ;
  • Koh, Chang-Sung (Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University) ;
  • Shin, Taekyun (Department of Veterinary Medicine, College of Agriculture and Life Sciences, Cheju National University)
  • 김희철 (제주대학교 농업생명과학대학 수의학과) ;
  • 주홍구 (제주대학교 농업생명과학대학 수의학과) ;
  • 문창종 (제주대학교 농업생명과학대학 수의학과) ;
  • 안미정 (제주대학교 농업생명과학대학 수의학과) ;
  • 지영흔 (제주대학교 농업생명과학대학 수의학과) ;
  • 임윤규 (제주대학교 농업생명과학대학 수의학과) ;
  • 고창성 ;
  • 신태균 (제주대학교 농업생명과학대학 수의학과)
  • Accepted : 2004.06.14
  • Published : 2004.09.30
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Abstract

The aim of this study was to evaluate the expression of galectin-3, one of beta-galactoside-binding proteins, in the experimental autoimmune encephalomyelitis(EAE) model of Lewis rats or non-obese diabetic (NOD) mice. Western blot analysis showed that galectin-3 was weakly expressed in the spinal cords of complete Freund's adjuvant(CFA) immunized control rats. In EAE, however, galectin-3 expression was significantly increased at the peak stage(days 14 post-immunization), while it was decreased slightly at the recovery stage(day 21 post-immunization). Immunohistochemical analysis showed that galectin-3 was detected in some macrophages in demyelinating lesions of NOD mice, while galectin-3 was immunoreacted in some inflammatory cells in the perivascular cuffing in rat EAE lesions. Collectively, it is postulated that the expression of galectin-3 is significantly increased in response to neuroimmunological stimulation in the central nervous system, whereas it is weak in normal rats and mice.

Keywords

References

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