Preservative Effect of Soybean Sprouts Pre-soaked and Cultivated in the Solution of Natural Antimicrobial Mixture

천연 항균복합제재용액을 침지 및 재배용수로 처리한 콩나물의 선도유지 효과

  • 정준호 (경상대학교 농업생명과학연구원) ;
  • 조성환 (경상대학교 식품공학과)
  • Published : 2004.03.01

Abstract

The antimicrobial effect of mixed solution of botanical antimicrobial agent-citrus product (BAAC) and such a natural additive as aloe, ginseng or Prunus mume extract on the spoilage microorganisms of soybean sprouts were investigated by paper disk method. The mixture (BAAG) of BAAC and ginseng extract showed the remarkable antimicrobial activity in the result of the experiment. Therefore, we used BAAG as pre-soaking and cultivating solution of soybean sprouts. Total bacterial and Escherichia coli cell count of soybean sprouts pre-soaked and cultivated in the BAAG-diluted solution (50 ppm) showed 2.5 ${\times}$ 10 CFU/mL and 1.3 ${\times}$ 10 cfu/mL in comparison with 3.5 ${\times}$ 10$^3$ cfu/mL and 5.8 ${\times}$ 10$^3$ cfu/mL of the control, respectively. BAAG-treated soybean sprouts also showed no slimy brown product and undesirable odor characterized in the control. It was confirmed that BAAG could be a proper pre-soaking and cultivating solution of soybean sprouts.

본 실험에서는 식품의 미생물작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(botanical antimicrobial agent)에 천연보조제(인삼추출물, 알로에, 매실추출물)를 첨가하여 paper disk법에 의한 항균성 검사를 실시한 결과, 인삼추출물을 첨가한 천연복합항균제재(BAAG)에서 생육저해환이 가장 크고 뚜렷하게 나타났다. 한편, 천연 항균복합제재 용액에 콩나물용 콩을 농도별로 각각 침지 처리하여 냉장 및 상온에 저장하면서 대장균수, 총균수의 변화를 살펴보고, 외관상의 변화에 미치는 영향을 수도수로 침지처리한 대조구와 비교하면서 조사하였다. 실험 결과, 대장균은 BAAG의 침지처리에 의해 성장이 억제되었으며 처리농도가 증가시킬수록 균증식 억제 효과가 뚜렷하였고, 총균수도 BAAG 처리구의 콩나물이 가장 낮은 수치를 나타내었다. 외관상의 변화와 상품 가치를 측정한 결과는 수도수처리구인 대조구 콩나물이 저장 3일 후부터 부패취와 함께 표피의 점질성 갈변이 형성되어 상품가치가 크게 떨어진 상태였다. 위와 같은 결과, BAAG용액 속에서 콩나물 콩을 발아시켜 침지 처리함으로써 콩나물의 유통기간을 상당기간 연장할 수 있을 것으로 기대된다.

Keywords

References

  1. Yang, C.B., Lee, S.W., Ko, Y.S. and Yoon, S.K. (1979) Studies on the effective utilization of soybean. J. Korean Soc. Food and Nutr., 8, 1-8
  2. Kim, S.D., Jang, B.H., Kim, H.S., Ha, K.H., Kang, K.S. and Kim, D.H. (1982) Studies on the changes in chlorophyll, free amino acid and vitamin C content of soybean sprouts during circulation periods. Korean J. Nutr. and Food, 11, 57-62
  3. Choi, H.D., Kim, S.S., Kim, K.T., Lee, J.Y. and Park, W.M. (2000) Effect of presoaking treatments on growth and rot of soybean sprouts. Korean J. Food Sci. Technol., 32, 584-589
  4. Choi, H.D., Kim, S.S., Kim, S.R. and Lee, B.Y. (2000) Effect of irrigating solutions on growth and rot of soybean sprouts. Korean J. Food Sci. Technol., 32, 1122-1127
  5. Varoquaux, P., Albagnac, G., Nguyenthe, C. and Varoquaux, F. (1996) Modified atmosphere packaging of fresh bean sprouts. J. Sci. Food Agric., 70, 224-228 https://doi.org/10.1002/(SICI)1097-0010(199602)70:2<224::AID-JSFA487>3.0.CO;2-0
  6. Park, W.P., Cho, S.H., and Lee, D.S. (1998) Effect of minima processing operations on the quality of garlic, green onion, soybean sprouts and watercress. J. Sci. Food Agric., 77, 282-286 https://doi.org/10.1002/(SICI)1097-0010(199806)77:2<282::AID-JSFA37>3.0.CO;2-4
  7. Park, W.M., Myung, I.S. and Lee, Y.S. (1986) Biological control against rot of soybean sprouts. Korean Soybean Digest, 3, 4-9
  8. Park, E.H., and Chopi, Y.S. (1995) Selection of useful chemicals reducing soybean sprouts rot. Korean J. Crop Sci., 40, 487-493
  9. Shigezo, N. and Ichizo, S. (1989) Effect of ozone treatment on elongation of hypocotyl and microbial counts of bean sprouts. Nippon Shokuhin Kogyo Gakkaishi, 36, 181-188 https://doi.org/10.3136/nskkk1962.36.3_181
  10. Lee, Y.S., Park, R.D. and Rhee, C.O. (1999) Effect of chitosan treatment on growing characteristics of soybean sprouts. Korean J. Food Sci. Technol., 31, 153-157
  11. Park, W.P., Cho, S.H. and Lee, D.S. (1998) Effect of grapefruit seed extract and ascorbic acid on the spoilage microorganisms and keeping quality of soybean sprouts. J. Korean Soc. Food Sci. Nutr., 27, 1086-1093
  12. lung, J.H. and Cho, S.H. (2003) Antimicrobial and antioxidant effect of botanical antimicrobial agent-citrus product on Pollack or Ascidian fishrneat. Korean J. Food Preservation, 10, 401-405
  13. Jung, J.H. and Cho, S.H. (2003) Effect of Steeping treatment in the natural antimicrobial agent solution on the quality control of processed tofu. Korean J. Food Preservation, 10, 41-46