Optimum Condition for Removing Bitter Substance of Yuzu(Citrus junos) by Enzyme Treatment

효소처리에 의한 유자 고미성분 제거의 최적 조건 연구

  • Published : 2004.03.01

Abstract

The content of naringin and hesperidin of Yuzu were 95.54 and 103.99 in peel ; 65.77 and 77.18 in flesh ; 16.49 and 15.88mg% in seed, respectively. When 10 mg% of naringin and 5 mg% hesperidin were treated with 10.0 units naringinase and 2.0 units of hesperidinase, they were decreased to 0.11 and 0.45 mg%, respectively. One percent of Japanese naringinase digested naringin and hesperidin that their final concentration were 0.54 and 0.09 mg% in 30 minutes, while 5% Amorepacific enzyme did until 0.26 and 0.04 mg%, respectively.

유자의 부위별 narinign 및 hesperidin의 함량은 과피가 95.54 mg%, 103.99 mg%, 과육이 65.77 mg%, 77.18 mg%, 씨에는 16.49 mg%, 15.88 mg% 순으로 검출되었다 유자에서 고미의 원인 물질인 naringin을 제거하기 위한 naringinase 최적 첨가량을 검토한 결과, naringinase 20 units 첨가구의 경우 90분 경과후 대부분 분해되었다. 표준시약으로 naringin 10 mg%와 hesperidin 5 mg%의 기질에 naringinase 10.0 units와 hesperidinase 2.0 units를 각각 첨가하여 측정한 결과 0.11 mg%와 0.45 mg%로 감소하였고, naringinase 1% 처리구에서 0.54 mg% 와 0.09 mg% 감소하였으며, 탁주용 당화효소 5% 처리구에서는 0.26 mg%, 0.04 mg%로 감소하였다.

Keywords

References

  1. 정동효 (1987) 효소학. 선진문화사, 서울. p.147-149
  2. Chung, J.H. (1972) Studies on contents of amino acid in Citrus junos Sieb. J. Korean Soc. Agric. Chem. Biotechnol., 15, 175-180
  3. Chung, J.H. (1974) Studies on the chemical compositions of Citrus junos in Korea. J. Korean Soc. Agric. Chem. Biotechnol., 17, 63-80
  4. Chung, J.H. (1972) Studies on the chemical components of Citrus junos sieb and physical and chemical properties of Citrus junos sieb. J. Korean Soc. Agric. Chem. Biotechnol., 15, 169-173
  5. Jeong, J.W., Park, K.J., Jung, S.w. and Kim, J.H. (1995) Changes in quality of citron juice by stomge and extraction conditions. J. Korean Soc. Agric. Chem. Biotechnol., 38, 141-146
  6. Jeong, J.W., Kwon, D.J., Hwang, J.R. and Jo, Y.J. (1994) Influence of the extraction method on quality of citron juice. Korean J. Food Sci. Technol., 26, 704-708
  7. Jeong, J.W., Lee, Y.C., Jung, S.w. and Lee, K.M. (1994) Flavour components of citron juice as affected by the extraction method. Korean J. Food Sci. Technol., 26, 709-712
  8. Rhyu, M.R., Kim, E.Y., Bae, I.Y. and Park, Y.K. (2002) Contents of nanngm, hesperidin and neohesperidin in premature Korea Citrus fruits. Korean J. Food Sci. Technol, 34, 132-135
  9. Tanaka, M., Aoki, T, Nakano, K. and Tanioka, H. (1992) Effect of temperature pretreatment on Yuzu (Citrus junas Sieb.ex Tanaka) fruits before low temperature storage. Bull.kochi Agric. Res. Ctr., 1, 85-89
  10. Masayo, K. and Ryonosuke, S. (1987) Seasonal change in color and carotenoid composition of Yuzu( Citrus junas Tanasa) and Lisbon lemon(Citrus limon Burm.f.) peel. Nippon Shokuhin Kogyo Gakkaishi, 34, 28-32 https://doi.org/10.3136/nskkk1962.34.28
  11. Lam, L., K. T., Zhang, J. and Hasegawa, S. (1994) Citrus limonoid reduction of chemically induced tumorigenesis. Overview NOV., p.104-106
  12. Miller, E.G., Gonzales, S.A.P., Couvillion, A.M, Hasegawa, S. and Lam, K.T (1994) Citrus limonoids as inhibitors of oral carcinogensis. Overview NOV., p.110-111
  13. Fumio H., Herman, Z. and Hasegawa, S. (1990) Limonoids in seeds of Yuzu, Nippon Shokuhin Kogyo Gakkaishi, 37, 380-383 https://doi.org/10.3136/nskkk1962.37.5_380
  14. Herman, Z., Fong, C.H., Ou, P. and Hasegawa, S. (1990) Limonoid glucosides in orange juices by HPLC. J. Agric. Food Chem., 38, 1860-1864 https://doi.org/10.1021/jf00099a016
  15. Bracke, M.E., Bruyneel, E.A., Vermeulen, S.J. and Mareel, M.M. (1994) Citrus lavonoid effect on tumor invasion and metastasis. Overview NOV., p.121-123
  16. James, F.F. and Wheaton, T.A (1976) A High pressure liquid chromatographic method for the resolution and quantity of naringin and naringenin retinoside in grapefruit juice. J. Agric. Chem., 24, 899-901