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Protective Effects of a Herb, Artemisia capillaris, Against Radiation-induced DNA Damage

방사선 유도 DNA 손상에 대한 인진쑥의 방어효과

  • Jo, Sung-Kee (Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Oh, Heon (Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Cheon, Eui-Hyun (Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Jeong, U-Hee (Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Cho, Nam-Jeong (School of Life Science, Chungbuk National University)
  • 조성기 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 오헌 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 천의현 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 정우희 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 조남정 (충북대학교 생화학과)
  • Published : 2004.01.01

Abstract

In the present study, the protective effects of Artemisia capillaris (AC) on the DNA damage induced by $^{60}$ Co ${\gamma}$-rays were evaluated using alkaline single-cell gel electrophoresis (SCGE, comet assay) in the mouse peripheral lymphocytes and micronuclei (MN) formation test in the Chinese hamster ovary (CHO) cells. We also investigated the effect of AC on 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation in the mouse liver and thymus exposed to ${\gamma}$-ray, The tail moment and the frequency of MN, which were markers of DNA damage in the SCGE and MN formation test, were decreased in the groups treated with AC extract before exposure to 200 cGy of ${\gamma}$-ray. We also observed its activities, lowering 8-OHdG level, an index of oxidative DNA damage, in the groups treated with AC extract before whole body ${\gamma}$-irradiation (800 cGy). It is plausible that scavenging of free radicals by AC may have played an important role in providing the protection against the radiation-induced damage to the DNA. These results indicated that AC protects the DNA damage induced by ${\gamma}$-rays and might be a useful radioprotector, especially since it is a relatively nontoxic product.

인진쑥의 방사선에 의한 산화적 손상에 대한 DNA 방어효과를 확인하기 위하여 마우스 림프구에서 단세포전기영동법과 CHO 세포에서 미소핵 형성 시험 그리고 마우스의 간과 흉선 조직의 DNA에서 8-OHdG의 형성정도를 관찰하였다. 그 결과 단세포전기영동법 및 미소핵 형성 시험에서는 50 $\mu\textrm{g}$/mL의 농도에서 가장 높은 DNA 손상 억제 효과를 나타내었으며, 8-OHdG 형성 정도는 간과 흉선 모두 30 mg/kg 농도 투여군에서 높은 방어효과를 나타내었다. 이상의 결과에서 인진쑥 추출물은 DNA 상해를 효과적으로 방어하였고 특히, 기존에 알려진 방사선 방호물질에 비하여 독성이 적은 천연물이라는 관점에서 방사선 방어제로 적용이 가능할 것으로 사료된다.

Keywords

References

  1. IAEA safety series No. 47. Manual on Early Medical Treatment of Possible Radiation Injury IAEA
  2. NCP report No. 65 Management of Persons Accidentally Contaminated with Radionuclides
  3. Science v.110 Cystein protects against x-irradiation Patt H.;Tyree M.;Straube,R.L. https://doi.org/10.1126/science.110.2852.213
  4. Cancer Res v.42 Protective effects of S-2-(3-aminopropylamino) ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice Milas L.;Hunter N.;Reid,B.O.;Thames,Jr.H.D.
  5. Pharmacol Ther v.39 Radioprotectors in tumor radiotherapy: Factors and settings determining therapeutic Milas L.;Murray D.;Brock,W.A.;Meyn,R.E. https://doi.org/10.1016/0163-7258(88)90059-9
  6. J Immunol v.136 Interleukin 1 is a radioprotector Neta R.;Douches S.;Oppenheim,J.J.
  7. Pharmacol Ther v.39 Role of cytokines in radioprotection Neta R. https://doi.org/10.1016/0163-7258(88)90070-8
  8. Int J Radiat Biol v.57 Therapeutic use of recombinant human G-CSF (rhG-CSF) in a canine model of sublethal and lethal whole body irradiation MacVittie,T.J.;Moruroy,R.L.;Patchen,M.L.;Souza,L.M. https://doi.org/10.1080/09553009014550891
  9. Radiat Res v.59 Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: dependence on tumor type, drug dose and species Washburn,L.C.;Carlton,J.E.;Hayes,R.L.
  10. Radiat Res v.94 Adverse effect of radioprotector WR2721 Cairnie,A.B. https://doi.org/10.2307/3575878
  11. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih v.12 Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy Zneg,X.L.;Li,X.A.;Zhang,B.Y.
  12. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih v.12 Clinical and experimental study on shen-qi injection with chemotherapy in the treatment of malignani tumor of digestive tract Li,N.Q.
  13. Chin Med J Engl v.104 Advances in the pharma-cological studies of radix Angelica sinensis (Oliv) Diels (Chincese Danggui). Mei,Q.B.;Tao,T.Y.;Cui B.
  14. Exp Hematol v.16 Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation Miyanomae T.;Frindel E.
  15. Chung Hua I Hsueh Tsa Chih v.76 The effect of angelica polysaccharide on proliferation and differentiation of hematopoietic progenitor cell Wang Y.;Zhu B.
  16. Yakugaku Zasshi v.110 Studies on chemical protectors against radiation. XXX. Radioprotective substances of Cnidii rhizoma Ohta S.;Sakurai N.;Sato Y.;Inoue T.;Shinoda M.
  17. Am J Chin Med v.18 Radioprotective effect of Ganoderma lucidum (Leyss. ex. Fr.) Karst after X-ray irradiation in mice Hsu,H.Y.;Lian,S.L.;Lin,C.C. https://doi.org/10.1142/S0192415X90000095
  18. Chung Kuo Chung Yao Tsa Chih v.19 Effects of Radix Astragali on hemopoiesis in irradiated mice Quan,H..;Li,H.S.
  19. J Kor Soc Food Nutr v.21 Antimutagenic effect of the major volatile compounds identified from mugwort (Artemisia asiatica nakai) leaves Kim,J.O.;Kim,Y.S.;Lee,J.H.;Kim,M.N.;Lee,S.H.;Park,G.Y.
  20. MS Thesis. The Catholic Univ. of Korea Studies on the antimicrobial effect on mugwort (Artemisia asiatica nakai) leaves Lee,J.S.
  21. J Kor Soc Food Nutr v.21 Antioxidative effectiveness of water extract and ether extract in wormwood (Artemisia montana Pampan) Lee,G.D.;Kim,J.S.;Bae,J.O.;Yoon,H.S.
  22. Int J Radiat Biol v.66 Induction of DNA single-strand breaks in human oymphocytes by low doses of γ-ray Singh,N.P.;Stephens,R.E.;Schneider,E.L.
  23. J Clin Lab Invest v.21 Isolation of mononuclear cells and granulocytes from human blood Boyum A. https://doi.org/10.3109/00365516809076979
  24. Appl Radiat Isot v.38 The present state and perspectives of micronucleus assay in radiation protection (A review). Almassy Z.;Krepinsky,A.B.;Koteles,G.J. https://doi.org/10.1016/0883-2889(87)90033-5
  25. Biochem Biophys Res Comun v.137 Formation of 8-hydroxydeoxyguanosine, hydroxyl free radical adduct of DNA in granulocytes exposed to the tumor promoter, tetradecanoylphorbolacetate Floyd,R.A.;Watson,J.J.;Harris J.;West M.;Wong,P.K. https://doi.org/10.1016/0006-291X(86)91156-3
  26. Radiat Res v.59 Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: Dependence on tumor type, drug dose and species Washburn,L.C.;Carlton,J.E.;Hayes,R.L.
  27. Radiat Res v.94 Adverse effect of radioprotector WR 2721 Cairnie,A.B. https://doi.org/10.2307/3575878
  28. Chung Kuo Chung Yao Tsa Chih v.16 The absorption of Fe, Zn, Cu in siwu, sijunzi, and Liuwei dihuang decoction by small intestine in rats Lu G.;Yang M.;Shen Y.;Meng J.
  29. IN VIVO v.7 In vivo radioprotective activity of panax ginseng and diethyldithiocarbamate Kim,S.H.;Cho,C.K.;Yoo,S.Y.;Koh,K.H.;Yun,H.G.;Kim,T.H.
  30. Chin Med J Engl v.104 Advances in the pharmacological studies of radix Angelica sinensis (Oliv) Diels (Chiness Danggui) Mei,Q.B.;Tao,T.Y.;Cui B.
  31. J Korea Asso Radiat Prot v.27 Protective effects of Paeonia japonica against radiation-induced damage OH,H.;Park,H.R.;Jeong,I.Y.;Kim,S.H.;Jo,S.K.
  32. Yakugaku Zasshi v.96 Studies on "Inchinko" I.Capillarisin, a new choleretic substance Komiya T.;Tsukui M.;Oshio H.
  33. Korean J Food Sci Ani Resour v.19 Effects of mugwort-water extracts on cardmium toxicity in rats Lee,C.H.;Han,K.H.;Choi,I.S.;Kim,C.Y.;Cho,J.K.
  34. Arch Toxicol v.73 Protective effects of capillarisin on rert-butylhydroperoxide-induced oxidative damage in rat primary hepatocytes Chu,C.Y.;Tseng,T.H.;Hwang,J.M.;Chou,F.P.;Wang,C.J. https://doi.org/10.1007/s002040050615
  35. J Ethonophamacol v.85 Anti-oxidant activities of the extracts from the herbs of Artemisia apiacea Kim,K.S.;Lee,S.;Lee,Y.S.;Jung,S.H.;Park,Y.;Shin,K.H.;Kim,B.K. https://doi.org/10.1016/S0378-8741(02)00338-0
  36. Radiat Res v.111 Radiation protection of murine intestine by WR-2721, 16, 16-dimethyl prostaglandin E₂ and the combination of both agents Hanson,W.R. https://doi.org/10.2307/3576992
  37. Radiat Res v.153 Radioprotective thiolamines WR-1065 and WR-33278 selectively denature nonhistone nuclear proteins Booth,V.K.;Roberts,J.C.;Warters,R.L.;Wilmore,B.H.;Lepock J. https://doi.org/10.1667/0033-7587(2000)153[0813:RTWAWS]2.0.CO;2

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