Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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A Study of Medicinal Plants for Applications in Functional Foods 1. Effects of Schizandrae fructus on the Regional Cerebral Blood Flow and Blood Pressure in Rats

기능성 식품으로의 활용을 위한 한약자원에 관한 연구 1. 오미자 열수추출물이 흰쥐의 국소 뇌혈류량과 혈압에 미치는 영향

  • 박성혜 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 한종현 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2004.01.01
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Abstract

The purpose of this study was to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) in rats, following the intravenous injection of Schizandrae fructus water extract. The measurement was continually monitored by laser-doppler flowmeter and pressure tranducer in anesthetized adult Sprague-Dawley rats for 2 hours to 2 hours and a half through the data acquisition system composed of MacLab and Macintosh computer. The result of this experiment was as followed. Schizandrae fructus increased the changes of rCBF in rats significantly. The rCBF of Schizandrae fructus did not change by pretreated propranolol, atropine, L-NNA and indomethacin. But the rCBF of Schizandrae fructus was increased by pretreated methylene blue. Schizandrae fructus decreased the changes of BP, significantly. The BP of Schizandrae fructus did not change by pretreated propranolol, atropine, L-NNA and indomethacin. But the BP of Schizandrae fructus was decreased by pretreated methylene blue. There results indicated that Schizandrae fructus can increase the rCBF and decrease the BP, that is related to guanylyl cyclase activity.

본 연구는 기능성 식품으로의 활용을 위한 한약자원에 관한 연구의 일환으로 계획되었고 선행연구와 문헌상의 기록을 바탕으로 한 오미자의 여러 기능 중 혈류개선효과에 중점을 두고 그 기능을 과학적으로 확인하여 보고자 하였다. 백서를 대상으로 LDF를 이용하여 오미자의 국소 뇌혈류량과 혈압에 대한 효능을 관찰한 결과는 다음과 같다. 오미자를 여러 농도로 투여시 국소 뇌혈류량은 증가되었다 오미자가 국소 뇌혈류량을 증가시키는 기전을 확인하기 위해 propranolol, atropine, L-NNA, indomethacin을 전처리한 후 오미자를 투여 했을 때는 국소 뇌혈류량에 유의한 변화를 관찰할 수 없었으나 methylene blue를 전처리한 후 오미자를 1.0 mg/kg 농도 이상으로 투여했을 때는 국소 뇌혈류량이 유의적으로 증가함을 관찰할 수 있었다. 오미자를 여러 농도로 투여시 혈압은 하강되었다. 오미자가 어떤 기전으로 혈압의 변화를 초래하는지를 확인하기 위해 propranolol, atropine, L-NNA, indomethacin을 전처리한 후 오미자를 투여했을 때는 혈압하강에 유의한 변화를 관찰할 수 없었으나 methylene blue를 전처리한 후 오미자를 1.0 mg/kg 농도이상으로 투여했을 때에는 혈압이 유의적으로 하강하는 변화가 나타났다. 이상의 결과에서 오미자는 뇌혈류량을 증가시키고 혈압을 강하시키는 작용이 있음을 확인하였고 그 기전은 guanylyl cyclase의 활성을 변화시킴으로써 나타나는 결과로 판단된다. 따라서 연구의 목적인 기능성 식품으로의 활용에 있어 원재료로 사용이 가능하다고 판단되며 향후 오미자를 이용한 기능성 식품의 제조 및 완제품에 대한 기능성을 평가하여 기능성 식품시장이 올바르게 정립되는데 기여하고자 한다.

Keywords

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