Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Uncoupling Protein 3 in the Rainbow Trout, Oncorhynchus mykiss Sequence, Splicing Variants, and Association with the AvaIII SINE element

  • Kim, Soon-Hag (National Genome Research Institute, NIH) ;
  • Choi, Cheol-Young (Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institute of Health) ;
  • Hwang, Joo-Yeon (National Genome Research Institute, NIH) ;
  • Kim, Young-Youl (National Genome Research Institute, NIH) ;
  • Park, Chan (National Genome Research Institute, NIH) ;
  • Oh, Berm-Seok (National Genome Research Institute, NIH) ;
  • Kimm, Ku-Chan (National Genome Research Institute, NIH) ;
  • Scott A. Gahr (Division of Animal and Veterinary Sciences, Davis College of Agriculture, Forestry and Consumer Sciences, West Virginia University) ;
  • Sohn, Young-Chang (Division of Marine Life Sciences, Kangnung National University)
  • Published : 2004.02.01
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Abstract

A rainbow trout uncoupling protein 3 (UCP3) cDNA clone, encoding a 310 amino acid protein, was cloned and sequenced from a liver cDNA library. Two different splice variants designated UCP3-vl and UCP3-v2, were identified through liver cDNA library screening using rainbow trout UCP3 cDNA clone as a probe. UCP3-vl has 3 insertions in the UCP3 cDNA: the first insertion (133 bp), the second (141 bp), and the third (370 bp) were located 126 bp, 334 bp and 532 bp downstream from the start codon, respectively. UCP3-v2 contained a single insertion, identical in sequence and location to the second insertion of UCP3-vl. UCP3, a mitochondrial protein, functions to modulate the efficiency of oxidative phosphorylation. UCP3 has been detected from heart, testis, spinal cord, eye, retina, colon, muscle, brown adipose tissue and white adipose tissue in mammalian animals. Human and rodent UCP3s are highly expressed in skeletal muscle and brown adipose tissue, while they show weak expression of UCP3 in heart and white adipose tissue. In contrast to mammalian studies, RT-PCR and Southern blot analysis of the rainbow trout demonstrated that UCP3 is strongly expressed in liver and heart. UCP3, UCP3-vl, and UCP3-v2 all contain an Ava III short interspersed element (SINE), located in the 3'untraslated region (UTR). PCR using primers from the Ava III SINE and the UCP3 3'UTR region indicates that the UCP3 cDNA is structurally conserved among salmonids and that these primers may be useful for salmonid species genotyping.

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