한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제49권1호
  • /
  • Pages.30-35
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  • 2004
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
권호 표지

Phytotoxic Effect of Xanthium occidentale Leaf Extract on Seed Germination and Early Seedling Growth of Alfalfa and Barnyard Grass

  • Chon, Sang-Uk (Biotechnology Industrialization Center, Dongshin University)
  • 발행 : 2004.03.01
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초록

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous extracts from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine their allelopathic effects, and the result showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Untreated seeds germinated in 60h, but extract concentrations greater than 30g $\textrm{L}^{-1}$ delayed seed germination. The extracts significantly inhibited seed germination of alfalfa, and $\beta$-amylase activity of alfalfa and barley seeds during 24-36 hours after treatment. Aqueous extracts of 40 g $\textrm{L}^{-1}$ from X. occidentale were completely inhibited the hypocotyl and root growth of alfalfa. Aqueous leaf extracts showed the highest inhibitory effect and followed by root and stem extracts. Early seedling growth of both alfalfa and barnyard grass (Echinochloa crus-galli) was significantly reduced by methanol extracts. By means of high-performance liquid chromatography, chlorogenic acid and trans-cinnamic acid were quantified as the highest amounts from water and EtOAc fractions, respectively. BuOH and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did hexane and water fractions. The findings of the bioassays for aqueous or methanol extracts reflected that the inhibitory effect of extract was closely related to the level of responsible allelochemicals found in plant extracts.

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참고문헌

  1. Banwart, W. L., P.M. Porter, T. C. Granato, and J. J. Hassett. 1985. HPLC separation and wavelength area ratios of more than 50 phenolic acids and flavonoids. J. Chern. Ecol. 11 : 383-395 https://doi.org/10.1007/BF01411424
  2. Ben-Hammouda, M., R. J. Kremer, and H. C. Minor. 1995. Phytotoxicity of extracts from sorghum plant components on wheat seedlings. Crop Sci. 35 : 1652-1656 https://doi.org/10.2135/cropsci1995.0011183X003500060023x
  3. Bendall, G. M. 1975. The allelopathic activity of California thistle (Circium arvense) in Tasmania. Weed Res. 15 : 77-81 https://doi.org/10.1111/j.1365-3180.1975.tb01102.x
  4. Bernfeld, P. 1955. Enzymes of carbohydrate metabolism. In: Methods in Enzymology. Academic Press, pp. 149-159
  5. Blum, U., T. R. Wnetworth, K. Klein, A. D. Worsham, L. D. King, T. M. Gerig, and S. W. Lyu. 1991. Phenolic acid content of soils from wheat-no till, wheat conventional till, and fallow-conventional till soybean cropping systems. J. Chem. Ecol. 17 : 1045-1068 https://doi.org/10.1007/BF01402933
  6. Chon, S. U. and C. J. Nelson. 2001. Effects of experimental procedures and conditions on bioassay sensitivity of alfalfa autotoxicity. Comm. Soil Sci. Plant Anal. 32 : 1607-1619 https://doi.org/10.1081/CSS-100104216
  7. Chon, S. U., J. H. Coutts, and C. J. Nelson. 2004. Osmotic and autotoxic effects of leaf extracts on germination and seedling growth of alfalfa. Agronomy J. (Accepted)
  8. Chou, C. H. and L. L. Leu. 1992. Allelopathic substances and interactions of Delonix regia (BOJ) Raf. J. Chem. Ecol. 18 : 2285-2303 https://doi.org/10.1007/BF00984951
  9. Chung, I. M. and D. A. Miller. 1995. Effect of alfalfa plant and soil extracts on germination and seedling growth. Agron. J. 87 : 762-767 https://doi.org/10.2134/agronj1995.00021962008700040025x
  10. Einhellig, F. A. and M. L. Stille. 1979. Effects of ferulic and p-coumaric acids on plant water status. Abstract. Bot. Soc.Am., Misc. Ser. Publ. No. 157 : 40-41
  11. Einhellig, F. A., E. L. Rice, P. G. Risser, and S. H. Wender. 1970. Effects of scopoletin on growth, $CO_2$ exchange rates, and concentration of scopoletin, scopolin, and chlorogenic acids in tobacco, sunflower, and pigweed. Bull. Torrey Bot. Club 97 : 22-33 https://doi.org/10.2307/2483987
  12. Guenzi, W. D. and T. M. McCalla. 1966. Phenolic acids in oats, wheat, sorghum, and corn residues and their phytotoxicity. Agron. J. 58 : 303-304 https://doi.org/10.2134/agronj1966.00021962005800030017x
  13. Inam, B., F. Hussain, and B. Farhat. 1987. Allelopathic effects of Pakistani weeds: Xanthium strumarium L. Pakistan Journal of Scientific and Industrial Research 30 : 530-533
  14. Kanchan, S. D. and Jayachandra. 1979. Allelopathic effects of Parthenium hysterophorus L. Ⅲ. Inhibitory effects of weed residues. Plant and Soil 53 : 37-47 https://doi.org/10.1007/BF02181877
  15. Mersie, W. and M. Singh. 1987. Allelopathic effects of parthenium (Parthenium hysterophorus L.) extract and residue on some agronomic crops and weeds. Journal of Chemical Ecology 13 : 1739-1747 https://doi.org/10.1007/BF00980214
  16. Miller, D. A. 1996. Allelopathy in forage crop systems. Agron. J. 88 : 854-859 https://doi.org/10.2134/agronj1996.00021962003600060003x
  17. National Academy of Sciences. 1971. Biochemical interactions among plants. Natil. Acad. Sci., Washington, DC. 134 pp
  18. Research Institute of Natural Products. 1996. Current Research Status of Natural Products in Korea. Seoul National University Press, Seoul, Korea
  19. Rice, E. L. 1984. Allelopathy. 2nd ed. Academic Press, New York, USA
  20. Stachon, W. J. and R. L. Zimdal. 1980. Allelopathic activitity of Canada Thistle (Circium arvense) in Colorado. Weed Sci. 28 : 83-86