DOI QR코드

DOI QR Code

Aspergillus nidulans 색소결핍 억제돌연변이주의 세포벽 미세구조

Ultrastructure of Cell Wall in the Suppressor Mutant of Null Pigmentation (SU-NPG) of Aspergillus nidulans


초록

A. nidulans의 색소결핍 억제돌연변이주(Suppressor mutant of the null pigmentation; SU-NPG)의 세포벽 구조가 색소형성 및 균사분지에 미치는 영향을 조사하여 다음의 결과를 얻었다. SEM으로 세포 표면구조를 관찰한 결과, 완전배지에서 배양된 SU-NPG의 분생포자벽은 최외각 층이 6일 째부터 박리되었으며, 균사생장이 저조하였다 TEM으로 관찰한 결과, 균사 세포벽은 매우 두꺼워졌다. 이러한 전자현미경관찰 결과는 이들 돌연변이주의 세포벽 구조가 NPG 및 야생형의 그것과는 다르다는 것을 시사한다. 균사에 탄수화물 염색을 한 후, 세포화학적 미세구조를 관찰한 결과, 분생포자벽은 C1, C2, C3와 C4 층으로 되어 있었고, 균사 세포벽은 H1, H2, H3와 H4층으로 되어 있었다. 이러한 C3층과 H3층은 세포분화 시 색소형성 및 균사 분지형성에 필수적인 세포벽 구조임 을 시사한다. SU-NPG는 FGSC4에서와는 다르게 비정상적인 균사생장을 하였지만, 색소결핍 돌연변이를 억제하는데, 이는 SU-NPG의 균사 세포벽에는 H3층이 형성되었으며, 세포벽 형성과정은 정상적으로 진행되기 때문으로 사료된다.

To investigate the effect of the cell wall on the pigmentation and branching in Aspergillus nidulans, ultrastructure of cell wall in suppressor mutant of the null pigmentation (SU-NPG, SU602) has been examined. Scanning electron microgrphs (SEM) revealed that the most outer layer of conidia wall peeled off in SU-NPG on day 6 from the complete conidiation. They also showed that hyphal growth and branching were not well developed in SU-NPG. Transmission electron micrographs (TEM) showed that the plasma membrane was not crenulated and the hyphal wall was thick in SU-NPG. These results indicated that the ultrastructure of cell wall in SU-NPC might be modified. Cytochemical analysis showed that the cell wall in SU-NPG was differentiated into Cl, C3, C2 and C4 layer in conidia and H1, H3, H2 and H4 layer in hyphae. C3 layer and H3 layer existed in SU-NPG. The increment of the diameter in SU-NPG hyphae might be caused by the thickness of H3 layer. These results suggest that SU-NPG may have an immature but the differentiated structure for the pigmentation in cell wall.

키워드

참고문헌

  1. J. Bacteriol. v.174 no.2 Melanin resistance of fungi to lysis Bloomfield,M.J.;M.Alexander
  2. Thesis for Ph.D. in Chonbuk National University Ultrastructure of the null pigmentation mutant (npg) in Aspergillus nidulans Chung,Y.S.
  3. Molecules and Cells v.6 no.6 Chemical composition and structure of hyphal wall of null pigmentation mutant of Aspergillus nidulans Chung,Y.S.;K.S.Chae;D.M.Han;K.Y.Jahng
  4. Journal of Microbiology v.41 no.3 Ultrastructure of the cell wall in null pigmentation mutation, npgA of Aspergillus nidulans Chung,Y.S.;K.M.Kim;D.M.Han;K.S.Chae;K.Y.Jahng
  5. Curr. Microbiol. v.16 Chemical composition and ultrastructure of wild-type and white mutant Aspergillus nidulans conidial walls Claverie-Martin, F.;M. R. Diaz-Torres;M.J.Geoghegan https://doi.org/10.1007/BF01568692
  6. Genetics v.64 The phenoloxidase of the ascomycete Podospora anserina. Ⅵ. Genetic regulation of the formation of laccase Esser,K.;W.Minuth
  7. Microbiol. Rev. v.54 no.4 To shape a cell: an inquiry into the causes of morphogenesis of microorganisms Harold,F.M.
  8. Mutation Res. v.48 Genetic damage induced by ethyl alcohol in Aspergillus nidulans Harsani,I.;I.A.Granek;D.W.Mackenzie
  9. Schizophyllum commune J. Bacteriol. v.106 Phenol oxidase activity and fruiting body formation Leonard,T.J.
  10. Appl. Environ. Microbiol. v.40 Degradation of melanin by Aspergillus fumigatus Luther,J.P.;H.Lipke
  11. J. Gen. Microbiol. v.73 Conidiophore and spore development in Aspergillus nidulans Oliver,P.T.P. https://doi.org/10.1099/00221287-73-1-45
  12. J. Cell. Biol. v.17 The use of lead citrate an high pH as an electron-opaque stain in electron microscopy Reynolds,E.S. https://doi.org/10.1083/jcb.17.1.208
  13. J. Gen. Microbiol. v.110 Determination of tip shape in fungal hyphae Saunders,P.T.;A.P.J.Trinci https://doi.org/10.1099/00221287-110-2-469
  14. Develop. Biol. v.138 Conidium Differntiation in Aspergillus nidulans wild type and wet-white (wetA) mutant strains Sewall,T.C.;C.W.Mims;W.E.Timberlake https://doi.org/10.1016/0012-1606(90)90215-5
  15. J. Submicrosc. Cytol. v.19 no.4 Improved Theiery staining for the ultrastructral detection of polysaccharides Silva,M.T.;P.M.Macedo
  16. Biochim. Biophys. Acta. v.273 Morphogenesis in Aspergillus nidulans: the significance of α-(1-3) glucan of the cell wall and α-(1-3) glucanase for cleistothcium development Zonneveld,B.J.M. https://doi.org/10.1016/0304-4165(72)90205-X
  17. J. Gen. Microbiol. v.81 α-(1-3) glucan synthesis, correlated with α-(1-3) glucanase synthesis, conidiation and fructification in morphogenetic mutants of Aspergillus nidulans Zonneveld,B.J.M. https://doi.org/10.1099/00221287-81-2-445